scholarly journals Molecular integrity of chloroplast ribosomal ribonucleic acid

1973 ◽  
Vol 135 (1) ◽  
pp. 237-240 ◽  
Author(s):  
Christopher J. Leaver
Keyword(s):  
Secondary Structure ◽  
Hydrogen Bonds ◽  
Ribonucleic Acid ◽  
Low Temperatures ◽  
Ribosomal Ribonucleic Acid ◽  
Polynucleotide Chain

The majority of chloroplast 1.1X106-mol.wt. rRNA molecules are nicked at specific points in the polynucleotide chain, the molecules being kept intact at low temperatures by their secondary structure. Conditions that break hydrogen bonds and lead to loss of secondary structure cause dissociation of the molecule.

scholarly journals A spectrophotometric study of the secondary structure of precursor ribosomal ribonucleic acid from Krebs ascites-tumour cells

1973 ◽  
Vol 135 (2) ◽  
pp. 349-351 ◽  
Author(s):  
A. A. Hadjiolov ◽  
R. A. Cox
Keyword(s):  
Secondary Structure ◽  
Ribonucleic Acid ◽  
18S Rrna ◽  
Spectrophotometric Study ◽  
Spectrophotometric Analysis ◽  
28S Rrna ◽  
Ascites Tumour ◽  
Helical Content ◽  
Ribosomal Ribonucleic Acid ◽  
Ascites Tumour Cells

The spectrophotometric analysis of 45S precursor rRNA shows that it contains more G and C residues than does mature 28S or 18S rRNA. The helical content and the length of double-helical segments in 45S and 28S rRNA are similar.

scholarly journals A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes

1976 ◽  
Vol 160 (3) ◽  
pp. 505-519 ◽  
Author(s):  
R A Cox ◽  
W Hirst
Keyword(s):  
Escherichia Coli ◽  
Secondary Structure ◽  
Ribonucleic Acid ◽  
Magnesium Ions ◽  
Base Pairs ◽  
E Coli ◽  
Ribosomal Ribonucleic Acid ◽  
The Stability ◽  
Rabbit Reticulocytes

Mg2+ was shown to affect the conformation of rRNA over the range of 0.03-1.2M-KCl. The species studies were Escherichia coli S-rRNA and L-rRNA (the RNA moieties of the smaller and larger subribosomal particles respectively) and rabbits S-rRNA and L-rRNA. 2. The addition of Mg2+ to rRNA in reconstitution buffer (0.35M-KCl0.01M-Tris/HCl, pH7.2) at 20° C let to an increase in bihelical secondary structure through the formation of additional (mainly A-U) base-pairs (e.g. an additional approx. 58 A-U base-pairs per molecule of E. coli S-rRNA as judged by u.v. difference spectrophotometry…

scholarly journals Studies on the 3′-terminal sequences of the large ribosomal ribonucleic acid of different eukaryotes and those associated with ‘hidden’ breaks in heat-dissociable insect 26S ribonucleic acid

1974 ◽  
Vol 141 (3) ◽  
pp. 617-625 ◽  
Author(s):  
John Shine ◽  
John A. Hunt ◽  
Lynn Dalgarno
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drosophila Melanogaster ◽  
Ribonucleic Acid ◽  
Galleria Mellonella ◽  
Terminal Sequence ◽  
6S Rna ◽  
Ribosomal Ribonucleic Acid ◽  
Higher Eukaryotes ◽  
26S Rrna ◽  
Polynucleotide Chain

The 3′-terminal sequences associated with the large rRNA complex from a range of eukaryotes were determined after pancreatic or T1-ribonuclease digestion of RNA terminally labelled with [3H]isoniazid. In all higher eukaryotes examined except Drosophila melanogaster, the 3′-terminal sequences Y-G-UOH and G-C-UOH were demonstrated for the large RNA component(s) and for 6S RNA respectively. The 3′-terminal sequence of Saccharomyces cerevisiae 26S RNA was Y-G-UOH and that of 6S RNA Y-A-U-U-UOH. Three 3′-terminal sequences were found in equimolar amounts in the heat-dissociable 26S rRNA characteristic of insect ribosomes. These were Y-G-U-G-UOH, Y-C-G-UOH and G-C-UOH for cultured Antheraea eucalypti cells, Y-G-UOH, Y-G-UOH and G-C-UOH for Galleria mellonella larvae and Y-C-G-AOH, Y-G-U-AOH and G-Y-U-GOH for Drosophila melanogaster flies. Thus the introduction of the central scission in insect 26S rRNA results in the generation of a unique 3′-terminus and does not arise from random cleavage of the polynucleotide chain.

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