Effect of point mutations on 5.8S ribosomal ribonucleic acid secondary structure and the 5.8S-28S ribosomal ribonucleic acid junction

Biochemistry ◽  
1981 ◽  
Vol 20 (14) ◽  
pp. 4029-4033 ◽  
Author(s):  
Thomas O. Sitz ◽  
Nandita Banerjee ◽  
Ross N. Nazar
Keyword(s):  
Secondary Structure ◽  
Ribonucleic Acid ◽  
Point Mutations ◽  
Ribosomal Ribonucleic Acid

scholarly journals A spectrophotometric study of the secondary structure of precursor ribosomal ribonucleic acid from Krebs ascites-tumour cells

1973 ◽  
Vol 135 (2) ◽  
pp. 349-351 ◽  
Author(s):  
A. A. Hadjiolov ◽  
R. A. Cox
Keyword(s):  
Secondary Structure ◽  
Ribonucleic Acid ◽  
18S Rrna ◽  
Spectrophotometric Study ◽  
Spectrophotometric Analysis ◽  
28S Rrna ◽  
Ascites Tumour ◽  
Helical Content ◽  
Ribosomal Ribonucleic Acid ◽  
Ascites Tumour Cells

The spectrophotometric analysis of 45S precursor rRNA shows that it contains more G and C residues than does mature 28S or 18S rRNA. The helical content and the length of double-helical segments in 45S and 28S rRNA are similar.

scholarly journals Molecular integrity of chloroplast ribosomal ribonucleic acid

1973 ◽  
Vol 135 (1) ◽  
pp. 237-240 ◽  
Author(s):  
Christopher J. Leaver
Keyword(s):  
Secondary Structure ◽  
Hydrogen Bonds ◽  
Ribonucleic Acid ◽  
Low Temperatures ◽  
Ribosomal Ribonucleic Acid ◽  
Polynucleotide Chain

The majority of chloroplast 1.1X106-mol.wt. rRNA molecules are nicked at specific points in the polynucleotide chain, the molecules being kept intact at low temperatures by their secondary structure. Conditions that break hydrogen bonds and lead to loss of secondary structure cause dissociation of the molecule.

scholarly journals A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes

1976 ◽  
Vol 160 (3) ◽  
pp. 505-519 ◽  
Author(s):  
R A Cox ◽  
W Hirst
Keyword(s):  
Escherichia Coli ◽  
Secondary Structure ◽  
Ribonucleic Acid ◽  
Magnesium Ions ◽  
Base Pairs ◽  
E Coli ◽  
Ribosomal Ribonucleic Acid ◽  
The Stability ◽  
Rabbit Reticulocytes

Mg2+ was shown to affect the conformation of rRNA over the range of 0.03-1.2M-KCl. The species studies were Escherichia coli S-rRNA and L-rRNA (the RNA moieties of the smaller and larger subribosomal particles respectively) and rabbits S-rRNA and L-rRNA. 2. The addition of Mg2+ to rRNA in reconstitution buffer (0.35M-KCl0.01M-Tris/HCl, pH7.2) at 20° C let to an increase in bihelical secondary structure through the formation of additional (mainly A-U) base-pairs (e.g. an additional approx. 58 A-U base-pairs per molecule of E. coli S-rRNA as judged by u.v. difference spectrophotometry…

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