scholarly journals The oestrogen-stimulated synthesis of heterogeneous nuclear ribonucleic acid in the uterus of immature rats

1973 ◽  
Vol 131 (4) ◽  
pp. 689-697 ◽  
Author(s):  
J. T. Knowler ◽  
R. M. S. Smellie
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Mode Of Action ◽  
Size Range ◽  
Early Response ◽  
Immature Rats ◽  
Nuclear Rna ◽  
Very High

An early response to the administration of oestradiol-17β to immature rats is the synthesis of uterine RNA of very high-molecular-weight. This RNA is shown to be heterogeneous nuclear RNA. Increased precursor incorporation into the heterogeneous nRNA is not confined to entities of precise molecular weight but appears to involve much of the size range of the species. These findings are discussed with respect to the mode of action of oestradiol.

scholarly journals Characterization of uterine heterogeneous nuclear ribonucleic acid and the effect of oestradiol-17β on its synthesis

1978 ◽  
Vol 172 (3) ◽  
pp. 587-593 ◽  
Author(s):  
S Aziz ◽  
J T Knowler
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Early Response ◽  
Oestrogen Treatment ◽  
Size Profile ◽  
Immature Rats ◽  
Nuclear Rna ◽  
Kinetics Of ◽  
Molecular Weight Fractions

An early response to the administration of oestradiol-17 beta to immature rats is a dramatic stimulation in the synthesis of uterine hnRNA (heterogenous nuclear RNA). High-molecular-weight fractions of the hnRNA were purified and subfractionated on poly(U)-Sepharose into fractions that differed in their poly(A) content and their size profile on polyacrylamide gels. Oestrogen treatment of the rats stimulated the synthesis of all three fractions of high-molecular-weight hnRNA, but the kinetics of synthesis, degree of stimulation and size distribution of the newly synthesize RNA differed in each fraction.

scholarly journals The detection of messenger ribonucleic acid sequences in heterogeneous nuclear ribonucleic acid fractions of the oestrogen-stimulated rat uterus

1980 ◽  
Vol 187 (1) ◽  
pp. 265-267 ◽  
Author(s):  
S Aziz ◽  
J T Knowler
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Mrna Sequence ◽  
Rat Uterus ◽  
Complementary Dna ◽  
Messenger Ribonucleic Acid ◽  
Complementary Sequences ◽  
Nuclear Rna

cDNA (complementary DNA) complementary to the abundant sequences of mRNA isolated from oestrogen-stimulated uterus was hybridized to polyadenylated and non-polyadenylated uterine hnRNA (heterogeneous nuclear RNA). High-molecular-weight polyadenylated hnRNA, isolated under denaturing conditions, was able to saturate the cDNA, but complementary sequences were low abundance when compared with homologous mRNA. The mRNA sequence content of the polyadenylated hnRNA increased considerably during oestrogen-induced growth of the uterus. Non-polyadenylated hnRNA also contained sequences complementary to the cDNA.

scholarly journals The Structure of High Molecular Weight Ribonucleic Acid in Solution

1961 ◽  
Vol 1 (7) ◽  
pp. 525-537 ◽  
Author(s):  
Serge N. Timasheff ◽  
J. Witz ◽  
V. Luzzati
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid

U1 small nuclear RNA-like sequences in human high molecular weight RNA

1984 ◽  
Vol 122 (2) ◽  
pp. 838-844 ◽  
Author(s):  
J.M. Capasso ◽  
M.A. Docherty ◽  
A. Ray ◽  
E.D. Kaplan ◽  
G.L. Eliceiri
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Small Nuclear Rna ◽  
Nuclear Rna

scholarly journals The multiple complexes formed by the interaction of platelet factor 4 with heparin

1980 ◽  
Vol 191 (3) ◽  
pp. 769-776 ◽  
Author(s):  
P E Bock ◽  
M Luscombe ◽  
S E Marshall ◽  
D S Pepper ◽  
J J Holbrook
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Chain Length ◽  
Salt Concentration ◽  
Human Platelet ◽  
Platelet Factor 4 ◽  
High Protein ◽  
Platelet Factor ◽  
The Stability ◽  
Very High

The anisotropy of the fluorescence of dansyl (5-dimethylaminonaphthalene-1- sulphonyl) groups covalently attached to human platelet factor 4 was used to detect the macromolecular compounds formed when the factor was mixed with heparin. At low heparin/protein ratios a very-high-molecular-weight compound (1) was formed that dissociated to give a smaller compound (2) when excess heparin was added. 2. A large complex was also detected as a precipitate that formed at high protein concentrations in chloride buffer. It contained 15.7% (w/w) polysaccharide, equivalent to four or five heparin tetrasaccharide units per protein tetramer. In this complex, more than one molecule of protein binds to each heparin molecule of molecular weight greater than about 6 × 10(3).3. The stability of these complexes varied with pH, salt concentration and the chain length of the heparin. The limit complexes found in excess of the larger heparins consisted of only one heparin molecule per protein tetramer, and the failure to observe complexes with four heparin molecules/protein tetramer is discussed.

Mixing of Carbon Black with Rubber. VI. Analysis of NR/SBR Blends

1988 ◽  
Vol 61 (4) ◽  
pp. 609-618 ◽  
Author(s):  
George R. Cotten ◽  
Lawrence J. Murphy
Keyword(s):  
Molecular Weight ◽  
Carbon Black ◽  
High Molecular Weight ◽  
Surface Area ◽  
Bound Rubber ◽  
Very High

Abstract The distribution of carbon black in NR/SBR blends was determined through the analysis of bound rubber. The NR/SBR blends were found to be very different from the previously studied SBR/BR compounds: these differences were assigned to mutual insolubility of the two polymers and a very high molecular weight of NR. In NR/SBR blends, it was found that changes in molecular weight of the polymer has no effect on the carbon black distribution in the blend. While the “activity” of carbon black did not affect the distribution, the loading of the black in NR decreased linearly with increasing surface area of the black. Approximately 35% of normal tread blacks (surface area 80–100 m2/g) was found in the NR phase. However, the bond between NR and carbon black is quite weak, and black continues to migrate into the SBR phase on prolonged mixing or during blending of NR and SBR masterbatches.

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