scholarly journals The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX

1973 ◽  
Vol 131 (2) ◽  
pp. 351-357 ◽  
Author(s):  
P. Whiteman
Keyword(s):  
Quantitative Determination ◽  
Experimental Evidence ◽  
Alcian Blue ◽  
Preceding Paper ◽  
Mgcl2 Concentration ◽  
Theoretical Considerations ◽  
Urinary Glycosaminoglycans

1. The effect of MgCl2 concentration on the interaction of Alcian Blue 8GX and glycosaminoglycans in the urine of patients with mucopolysaccharidosis was studied by using a new quantitative micro method for the measurement of Alcian Blue–glycosaminoglycan complexes. This provided a means of measuring the critical electrolyte concentrations of urinary glycosaminoglycans. 2. Theoretical considerations based on the preceding paper (Whiteman, 1973) and experimental evidence provided here show that Alcian Blue 8GX may be used for the direct quantitative determination of total urinary glycosaminoglycans. The method is simple, requires sample volumes of 50μl or less, and gives results comparable with those obtained by other more complicated methods.

A method for the quantitative determination of urinary glycosaminoglycans

1976 ◽  
Vol 70 (3) ◽  
pp. 427-432 ◽  
Author(s):  
Robert E. Hurst ◽  
Juanita M. Settine ◽  
Andrew E. Lorincz
Keyword(s):  
Quantitative Determination ◽  
Urinary Glycosaminoglycans

scholarly journals The quantitative measurement of Alcian Blue–glycosaminoglycan complexes

1973 ◽  
Vol 131 (2) ◽  
pp. 343-350 ◽  
Author(s):  
P. Whiteman
Keyword(s):  
Complex Formation ◽  
Quantitative Determination ◽  
Alcian Blue ◽  
Quantitative Measurement ◽  
Cationic Dye ◽  
Molecular Binding ◽  
Maximum Precipitation ◽  
Hurler’S Syndrome ◽  
Acid Glycosaminoglycans

1. A simple new quantitative micro method was developed to study the interaction of the cationic dye Alcian Blue 8GX and acid glycosaminoglycans under different conditions. After washing with ethanol the precipitated Alcian Blue–glycosaminoglycan complex was dissociated in Manoxol IB solution and the amount of bound dye measured spectrophotometrically. 2. Reaction profiles of complex-formation were determined in the presence of different concentrations of MgCl2 at pH5.8, and could be used to study the critical electrolyte concentrations of glycosaminoglycans. At least 50mm-MgCl2 was required to produce maximum precipitation of, and maximum uptake of, Alcian Blue by standard glycosaminoglycans. Maximum uptake of Alcian Blue by glycosaminoglycans in the urine of a patient with Hurler's syndrome required the presence of 25–50mm-MgCl2. 3. Under standard conditions of maximum interaction, calibration curves for the quantitative determination of a series of standard glycosaminoglycans in 20μl volumes were nearly linear over the range 1–10μg. 4. The technique was used to determine the molecular binding ratios of Alcian Blue to glycosaminoglycans under controlled conditions.

Quantitative determination of longitudinal and transverse cross-relaxation rates

1999 ◽  
Vol 96 (9/10) ◽  
pp. 1608-1615
Author(s):  
T. E. Malliavin ◽  
H. Desvaux ◽  
M. A. Delsuc
Keyword(s):  
Quantitative Determination ◽  
Cross Relaxation ◽  
Relaxation Rates

Quantitative Determination of Gallic acid and Cyanidin-3-O-Glucoside within Sumac Extracts by HPLC-MS/MS

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
M Koşar ◽  
F Göger ◽  
N Kırımer ◽  
KHC Başer
Keyword(s):  
Quantitative Determination ◽  
Gallic Acid

Quantitative determination of compound leads in Schiniopsis brasiliensis Engler

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
FHA Fernandes ◽  
RSA Batista ◽  
G Véras ◽  
FS Souza ◽  
ACD Medeiros
Keyword(s):  
Quantitative Determination
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