scholarly journals The quantitative measurement of Alcian Blue–glycosaminoglycan complexes

1973 ◽  
Vol 131 (2) ◽  
pp. 343-350 ◽  
Author(s):  
P. Whiteman
Keyword(s):  
Complex Formation ◽  
Quantitative Determination ◽  
Alcian Blue ◽  
Quantitative Measurement ◽  
Cationic Dye ◽  
Molecular Binding ◽  
Maximum Precipitation ◽  
Hurler’S Syndrome ◽  
Acid Glycosaminoglycans

1. A simple new quantitative micro method was developed to study the interaction of the cationic dye Alcian Blue 8GX and acid glycosaminoglycans under different conditions. After washing with ethanol the precipitated Alcian Blue–glycosaminoglycan complex was dissociated in Manoxol IB solution and the amount of bound dye measured spectrophotometrically. 2. Reaction profiles of complex-formation were determined in the presence of different concentrations of MgCl2 at pH5.8, and could be used to study the critical electrolyte concentrations of glycosaminoglycans. At least 50mm-MgCl2 was required to produce maximum precipitation of, and maximum uptake of, Alcian Blue by standard glycosaminoglycans. Maximum uptake of Alcian Blue by glycosaminoglycans in the urine of a patient with Hurler's syndrome required the presence of 25–50mm-MgCl2. 3. Under standard conditions of maximum interaction, calibration curves for the quantitative determination of a series of standard glycosaminoglycans in 20μl volumes were nearly linear over the range 1–10μg. 4. The technique was used to determine the molecular binding ratios of Alcian Blue to glycosaminoglycans under controlled conditions.

Separation of Vitamins D2 and D3 as Isotachysterols D2 and D3 by Gas-Liquid Chromatography

1968 ◽  
Vol 51 (4) ◽  
pp. 834-838
Author(s):  
A J Sheppard ◽  
Denis E Lacroix ◽  
A R Prosser
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Infrared Absorption ◽  
Quantitative Measurement ◽  
Acetyl Chloride ◽  
Internal Standard ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Absorption Data

Abstract A method for the quantitative determination of 0.5—20 μg vitamins D2 and D3 by gas-liquid chromatography is described. Vitamins D2 and D3 are completely isomerized to their respective isotachysterol isomers by acetyl chloride as demonstrated by ultraviolet and infrared absorption data. Dihydrotachysterol D2, isotachysterol D2, and isotachysterol D3 are completely resolved with a 3% JXR on 100-120 mesh Gas Chrom Q column packing. Calibration studies show that each compound exhibited a characteristic dose-response plot. Therefore, one isomer cannot be used as a direct internal standard for the quantitative measurement of the other isomer.

A Spectrophotometric Procedure, Using Carboxypeptidase A, for the Quantitative Measurement of Ochratoxin A

1976 ◽  
Vol 59 (1) ◽  
pp. 128-129
Author(s):  
Karl Hult ◽  
Sten Gatenbeck
Keyword(s):  
Quantitative Determination ◽  
Ochratoxin A ◽  
Quantitative Measurement ◽  
Carboxypeptidase A ◽  
Excitation Spectra ◽  
Fluorescence Excitation ◽  
Fluorescence Excitation Spectra ◽  
Ochratoxin Α ◽  
The Difference

Abstract In the method described, ochratoxin A is cleaved into ochratoxin α (free isocoumarin chromophore) and phenylalanine, using carboxypeptidase. Detection is based on the difference in fluorescence excitation spectra of ochratoxin A (380 nm, maximum) and ochratoxin α (340 nm, maximum). The quantitation of ochratoxin A is based on the loss of fluorescence intensity at 380 nm. The method has been used for the quantitative determination of as little as 4 μg ochratoxin A/kg barley and barley meal but it could be extended to other products.

Rapid Method for the Quantitative Determination of Serum Alkaline Phosphatase

1960 ◽  
Vol 6 (3) ◽  
pp. 269-275 ◽  
Author(s):  
Bernard klein ◽  
Prunella A Read ◽  
Arthur L Babson
Keyword(s):  
Alkaline Phosphatase ◽  
Quantitative Determination ◽  
Rapid Method ◽  
Quantitative Measurement ◽  
Serum Alkaline Phosphatase ◽  
Photometric Measurement

Abstract A new procedure is described for the simple, rapid, quantitative measurement of serum alkaline phosphatase. The procedure is based on the direct photometric measurement of phenolphthalein released from sodium phenolphthalein phosphate. The substrate is available in a convenient, stabilized form as a tablet containing buffer and substrate in optimum amounts for a single analysis. As many as 30 analyses can be performed in an hour.

scholarly journals The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX

1973 ◽  
Vol 131 (2) ◽  
pp. 351-357 ◽  
Author(s):  
P. Whiteman
Keyword(s):  
Quantitative Determination ◽  
Experimental Evidence ◽  
Alcian Blue ◽  
Preceding Paper ◽  
Mgcl2 Concentration ◽  
Theoretical Considerations ◽  
Urinary Glycosaminoglycans

1. The effect of MgCl2 concentration on the interaction of Alcian Blue 8GX and glycosaminoglycans in the urine of patients with mucopolysaccharidosis was studied by using a new quantitative micro method for the measurement of Alcian Blue–glycosaminoglycan complexes. This provided a means of measuring the critical electrolyte concentrations of urinary glycosaminoglycans. 2. Theoretical considerations based on the preceding paper (Whiteman, 1973) and experimental evidence provided here show that Alcian Blue 8GX may be used for the direct quantitative determination of total urinary glycosaminoglycans. The method is simple, requires sample volumes of 50μl or less, and gives results comparable with those obtained by other more complicated methods.

Quantitative Determination of Dyes in Textile Fibers

1976 ◽  
Vol 46 (7) ◽  
pp. 483-484 ◽  
Author(s):  
Krik Kissa
Keyword(s):  
Quantitative Determination ◽  
Cationic Dyes ◽  
Disperse Dyes ◽  
Cationic Dye ◽  
Textile Fibers ◽  
Polyester Fibers

The dye content of polyester fibers on fiber blends containing both disperse and cationic dyes is determined by extracting the disperse dyes with chlorobenzene, followed by extraction of the cationic dye with DMF containing 55 g/l. ZnCl2, 8 ml/l. conc HCl (37%), and 20 g/l. 2,6-di- t-butyl-4-methylphenol.

QUANTITATIVE MEASUREMENT OF METAL ION CONCENTRATION OF PROTEINS SEPARATED BY ELECTROPHORESIS

1996 ◽  
Vol 06 (01n02) ◽  
pp. 215-225 ◽  
Author(s):  
G. WEBER ◽  
D. STRIVAY ◽  
C. MENENDEZ ◽  
B. SCHOEFS ◽  
M. BERTRAND
Keyword(s):  
Content Analysis ◽  
Quantitative Determination ◽  
Gel Electrophoresis ◽  
Metal Ion ◽  
Metal Content ◽  
Quantitative Measurement ◽  
Polyacrylamide Gel Electrophoresis ◽  
Ion Concentration ◽  
X Ray

This communication is devoted to nature determination and quantification by PIXE of metals contained in proteins after their separation by PolyAcrylamide Gel Electrophoresis (PAGE). After the electrophoresis, the gel is dried and each track is scanned with a 2.5 MeV proton beam which triggers metal X-ray fluorescence and then, allows to determine the type of metals contained in an electrophoretic band. For quantitative determination of the amount of the metal contained inside the band, the characteristic X-ray peak area is compared with those obtained with polyacrylamide gels doped with the same metal. The normalization has been achieved by using RBS measurements on the gel itself. The procedure presented seems to be a very useful multielementary method for the metal content analysis and for the determination of the metal amounts inside proteins after their separation by electrophoresis. Furthermore it allows to check if metals remain bound to proteins.

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