scholarly journals Palmitoyl-coenzyme A synthetase. Mechanism of reaction

1973 ◽  
Vol 131 (2) ◽  
pp. 199-209 ◽  
Author(s):  
J. Bar–Tana ◽  
G. Rose ◽  
R. Brandes ◽  
B. Shapiro
Keyword(s):  
Fatty Acid ◽  
Chain Fatty Acid ◽  
Acid Activation ◽  
Long Chain Fatty Acid ◽  
Mechanism Of Reaction ◽  
Ping Pong ◽  
Fatty Acid Activation ◽  
Kinetics Of ◽  
Enzyme Intermediate ◽  
Long Chain

The mechanism of long-chain fatty acid activation catalysed by highly purified microsomal palmitoyl-CoA synthetase was investigated. The kinetics of the overall reaction were found to conform to the Bi Uni Uni Bi Ping Pong mechanism. 18O was transferred from [18O]palmitate to AMP and palmitoyl-CoA exclusively. The enzyme intermediate formed appeared to consist of enzyme-bound palmitate; this formation occurred only in the presence of ATP. However, the involvement of palmitoyl-AMP in the reaction catalysed by the purified enzyme has proved difficult to establish.

scholarly journals Microsomal palmitoyl-coenzyme A synthetase from rat liver. Partial and exchange reactions

1972 ◽  
Vol 129 (5) ◽  
pp. 1101-1107 ◽  
Author(s):  
J. Bar–Tana ◽  
G. Rose ◽  
B. Shapiro
Keyword(s):  
Fatty Acid ◽  
Microsomal Fraction ◽  
Fatty Acyl ◽  
Chain Fatty Acid ◽  
Acid Activation ◽  
Exchange Reactions ◽  
Long Chain Fatty Acid ◽  
Atp Formation ◽  
Fatty Acid Activation ◽  
Long Chain

The partial and exchange reactions of long-chain fatty acid activation were determined by using purified microsomal long-chain fatty acyl-CoA synthetase (EC 6.2.1.3). No significant ATP formation from palmitoyl-AMP and PPi, nor palmitoyl-AMP-dependent CoA disappearance could be demonstrated. Similarly, no palmitate-dependent [32P2]PPi–ATP exchange was catalysed by the pure enzyme. The above partial and exchange reactions were, however, catalysed by the parent microsomal fraction at a rate similar to that of the overall reaction. The implications of these results are discussed.

Adsorption kinetics of a fluorescent dye in a long chain fatty acid matrix

2011 ◽  
Vol 79 (5) ◽  
pp. 1642-1647 ◽  
Author(s):  
Syed Arshad Hussain ◽  
Soma Banik ◽  
S. Chakraborty ◽  
D. Bhattacharjee
Keyword(s):  
Fatty Acid ◽  
Adsorption Kinetics ◽  
Chain Fatty Acid ◽  
Fluorescent Dye ◽  
Long Chain Fatty Acid ◽  
Kinetics Of ◽  
Long Chain
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