Adsorption kinetics of a fluorescent dye in a long chain fatty acid matrix

Author(s):  
Syed Arshad Hussain ◽  
Soma Banik ◽  
S. Chakraborty ◽  
D. Bhattacharjee
1973 ◽  
Vol 131 (2) ◽  
pp. 199-209 ◽  
Author(s):  
J. Bar–Tana ◽  
G. Rose ◽  
R. Brandes ◽  
B. Shapiro

The mechanism of long-chain fatty acid activation catalysed by highly purified microsomal palmitoyl-CoA synthetase was investigated. The kinetics of the overall reaction were found to conform to the Bi Uni Uni Bi Ping Pong mechanism. 18O was transferred from [18O]palmitate to AMP and palmitoyl-CoA exclusively. The enzyme intermediate formed appeared to consist of enzyme-bound palmitate; this formation occurred only in the presence of ATP. However, the involvement of palmitoyl-AMP in the reaction catalysed by the purified enzyme has proved difficult to establish.


2000 ◽  
Vol 41 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Karin A. J.M. van der Lee ◽  
Michaël M. Vork ◽  
Johan E. De Vries ◽  
Peter H.M. Willemsen ◽  
Jan F.C. Glatz ◽  
...  

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