scholarly journals The uptake and efflux of glycine from rat cerebral-cortex slices

1971 ◽  
Vol 125 (1) ◽  
pp. 255-260 ◽  
Author(s):  
P. Joanny ◽  
E. Barbosa ◽  
H. Hillman ◽  
J. Corriol
Keyword(s):  
Cerebral Cortex ◽  
Rat Cerebral Cortex ◽  
Facilitated Diffusion ◽  
Glycine Uptake ◽  
Saturation Kinetics ◽  
Na Ions ◽  
Cortex Slices ◽  
Kinetics Of

The kinetics of the influx and efflux of radioactive l-glycine was studied in slices of rat cerebral cortex. The influx showed saturation kinetics and was inhibited by l-alanine. Influx was dependent on the presence of Na+ ions and a metabolizable substrate. The efflux of glycine was accelerated by alanine. It was concluded that carrier-mediated facilitated diffusion was the mechanism of glycine uptake by, and efflux from, cerebral slices.

scholarly journals Uptake of monosaccharides by guinea-pig cerebral-cortex slices

1969 ◽  
Vol 112 (3) ◽  
pp. 367-371 ◽  
Author(s):  
P. Joanny ◽  
J. Corriol ◽  
H. Hillman
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig ◽  
Diffusion Kinetics ◽  
Cerebral Tissue ◽  
Tissue Slices ◽  
Saturation Kinetics ◽  
Cortex Slices ◽  
Kinetics Of

By the use of 1mm-iodoacetate to inhibit glycolysis in guinea-pig cerebral tissue slices, the kinetics of the uptake of monosaccharides on transfer of tissue from 0° to 37° were studied. d-Ribose, d-galactose, d-mannose, l-sorbose, and d-fructose showed diffusion kinetics, whereas 2-deoxy-d-glucose, d-glucose, d-arabinose and d-xylose showed saturation kinetics.

scholarly journals Stimulation by acetylcholine of phosphatidylinositol labelling. Subcellular distribution in rat cerebral-cortex slices

1972 ◽  
Vol 126 (5) ◽  
pp. 1141-1147 ◽  
Author(s):  
Eduardo G. Lapetina ◽  
Robert H. Michell
Keyword(s):  
Cerebral Cortex ◽  
Specific Radioactivity ◽  
Neuronal Cell ◽  
Subcellular Fractionation ◽  
Rat Cerebral Cortex ◽  
Marker Enzymes ◽  
Fresh Tissue ◽  
Cell Structures ◽  
Neuronal Cell Bodies ◽  
Cortex Slices

1. Rat cerebral-cortex slices were incubated with 32Pi, acetylcholine and eserine for periods of 10min and 2h. The specific radioactivity of phosphatidylinositol was elevated during these treatments by 36 and 106% respectively. 2. The specific radioactivities of the phosphatidylinositol in different cell structures were determined after subcellular fractionation. They were highest in the nuclear, microsomal and synaptic-vesicle fractions and lowest in myelin, both in the controls and in the acetylcholine-treated slices. 3. The stimulated labelling of phosphatidylinositol was relatively evenly distributed: no subcellular fraction showed a stimulation markedly higher than that in the homogenate. 4. Studies of the distributions and activities of marker enzymes indicated that the subcellular fractionation achieved was similar to that with fresh tissue. 5. The results are discussed in relation to the previous report that the stimulation is observed throughout the neuronal cell-bodies and in relation to the hypothesis that the labelled phosphatidylinositol produced by stimulation is a component of an acetylcholine-receptor proteolipid localized in the synaptic junction.

Phosphorylation of MARCKS by endothelin in rat cerebral cortex slices

1999 ◽  
Vol 24 (3) ◽  
pp. 155-162 ◽  
Author(s):  
Mar�a J. P�rez-Alvarez ◽  
M. Carmen Calcerrada ◽  
R. Edgardo Catal�n ◽  
Ana M. Mart�nez
Keyword(s):  
Cerebral Cortex ◽  
Rat Cerebral Cortex ◽  
Cortex Slices
Sign in / Sign up

Export Citation Format

Share Document