scholarly journals Uptake of monosaccharides by guinea-pig cerebral-cortex slices

1969 ◽  
Vol 112 (3) ◽  
pp. 367-371 ◽  
Author(s):  
P. Joanny ◽  
J. Corriol ◽  
H. Hillman
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig ◽  
Diffusion Kinetics ◽  
Cerebral Tissue ◽  
Tissue Slices ◽  
Saturation Kinetics ◽  
Cortex Slices ◽  
Kinetics Of

By the use of 1mm-iodoacetate to inhibit glycolysis in guinea-pig cerebral tissue slices, the kinetics of the uptake of monosaccharides on transfer of tissue from 0° to 37° were studied. d-Ribose, d-galactose, d-mannose, l-sorbose, and d-fructose showed diffusion kinetics, whereas 2-deoxy-d-glucose, d-glucose, d-arabinose and d-xylose showed saturation kinetics.

scholarly journals The uptake and efflux of glycine from rat cerebral-cortex slices

1971 ◽  
Vol 125 (1) ◽  
pp. 255-260 ◽  
Author(s):  
P. Joanny ◽  
E. Barbosa ◽  
H. Hillman ◽  
J. Corriol
Keyword(s):  
Cerebral Cortex ◽  
Rat Cerebral Cortex ◽  
Facilitated Diffusion ◽  
Glycine Uptake ◽  
Saturation Kinetics ◽  
Na Ions ◽  
Cortex Slices ◽  
Kinetics Of

The kinetics of the influx and efflux of radioactive l-glycine was studied in slices of rat cerebral cortex. The influx showed saturation kinetics and was inhibited by l-alanine. Influx was dependent on the presence of Na+ ions and a metabolizable substrate. The efflux of glycine was accelerated by alanine. It was concluded that carrier-mediated facilitated diffusion was the mechanism of glycine uptake by, and efflux from, cerebral slices.

Effect of (-)nicotine on GABA efflux from guinea pig cerebral cortex slices

1989 ◽  
Vol 21 ◽  
pp. 133-134 ◽  
Author(s):  
P. Spalluto ◽  
M. Morari ◽  
L. Ferraro ◽  
A. Nordberg ◽  
T. Antonelli ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig ◽  
Cortex Slices

scholarly journals Accumulation of inositol polyphosphate isomers in agonist-stimulated cerebral-cortex slices. Comparison with metabolic profiles in cell-free preparations

1989 ◽  
Vol 258 (1) ◽  
pp. 23-32 ◽  
Author(s):  
I H Batty ◽  
A J Letcher ◽  
S R Nahorski
Keyword(s):  
Cerebral Cortex ◽  
Inositol Phosphates ◽  
Periodate Oxidation ◽  
Chemical Degradation ◽  
Inositol Polyphosphate ◽  
Tissue Slices ◽  
Inositol 1 ◽  
Agonist Stimulation ◽  
Cortex Slices ◽  
Hydrolysis Of

1. Basal and carbachol-stimulated accumulations of isomeric [3H]inositol mono-, bis-, tris- and tetrakis-phosphates were examined in rat cerebral-cortex slices labelled with myo-[2-3H]inositol. 2. In control samples the major [3H]inositol phosphates detected were co-eluted on h.p.l.c. with Ins(1)P, Ins(4)P (inositol 1- and 4-monophosphate respectively), Ins(1,4)P2 (inositol 1,4-bisphosphate), Ins(1,4,5)P3 (inositol 1,4,5-tris-phosphate) and Ins(1,3,4,5)P4 (inositol 1,3,4,5-tetrakisphosphate). 3. After stimulation to steady state with carbachol, accumulation of each of these products was markedly increased. 4. Agonist stimulation, however, also evoked much more dramatic increased accumulations of a second [3H]inositol trisphosphate, which was co-eluted on h.p.l.c. with authentic Ins(1,3,4)P3 (inositol 1,3,4-trisphosphate) and of three further [3H]inositol bisphosphates ([3H]InsP2(s]. 5. Examination of the latter by chemical degradation by periodate oxidation and/or h.p.l.c. allowed identification of these as [3H]Ins(1,3)P2, [3H]Ins(3,4)P2 and [3H]Ins(4,5)P2 (inositol 1,3-, 3,4- and 4,5-bisphosphates respectively), which respectively accounted for about 22%, 8% and 3% of total [3H]InsP2 in extracts from stimulated tissue slices. 6. By using a h.p.l.c. method which clearly resolves Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 (inositol 1,3,4,6-tetrakisphosphate), only the former isomer could be detected in extracts from either control or stimulated tissue slices. Similarly, [3H]inositol pentakis- and hexakis-phosphates were not detectable either in the presence or absence of carbachol under the radiolabelling conditions described. 7. The catabolism of [3H]Ins(1,4,5)P3 and [3H]Ins(1,3,4)P3 by cell-free preparations from cerebral cortex was also studied. 8. In the presence of Mg2+, [3H]Ins(1,4,5)P3 was specifically dephosphorylated via [3H]Ins(1,4)P2 and [3H]Ins(4)P to free [3H]inositol, whereas [3H]Ins(1,3,4)P3 was degraded via [3H]Ins(3,4)P2 and, to a lesser extent, via [3H]Ins(1,3)P2 to D- and/or L-[3H]Ins(1)P and [3H]inositol. 9. In the presence of EDTA, hydrolysis of [3H]Ins(1,4,5)P3 was greater than or equal to 95% inhibited, whereas [3H]Ins(1,3,4)P3 was still degraded, but yielded only a single [3H]InsP2 identified as [3H]Ins(1,3)P2. 10. The significance of these observations with cell-free preparations is discussed in relation to the proportions of the separate isomeric [3H]inositol phosphates measured in stimulated tissue slices.

Changes in Oxidative Metabolism of Guinea Pig Brain Cortex Slices in the Presence of Meso-Inositol Hexanicotinate and Nicotinic Acid

1973 ◽  
Vol 1 (3) ◽  
pp. 192-193 ◽  
Author(s):  
P Joanny ◽  
P Balansard ◽  
J Legros
Keyword(s):  
Cerebral Cortex ◽  
Oxygen Uptake ◽  
Guinea Pig ◽  
Protective Effect ◽  
Nicotinic Acid ◽  
Brain Cortex ◽  
Brain Cortex Slices ◽  
Cortex Slices ◽  
Guinea Pig Brain

Both nicotinic acid and meso-inositol hexanicotinate significantly decrease oxygen uptake of isolated guinea-pig cerebral cortex. This inhibition is tentatively correlated to the already described protective effect against hypoxia produced by these compounds in vivo.

Effect of γ-aminobutyric acid on cyclic nucleotides content of guinea-pig cerebral cortex slices

1984 ◽  
Vol 16 (9) ◽  
pp. 933-943
Author(s):  
T. Antonelli ◽  
F. Caciagli ◽  
L. Lambertini ◽  
E. Veratti ◽  
C. Bianchi
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig ◽  
Cyclic Nucleotides ◽  
Aminobutyric Acid ◽  
Cortex Slices
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