scholarly journals Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum

1970 ◽  
Vol 120 (3) ◽  
pp. 549-558 ◽  
Author(s):  
R. A. Cox ◽  
K. Kanagalingam ◽  
Elisabeth S. Sutherland
Keyword(s):  
Ribonucleic Acid ◽  
Penicillium Chrysogenum ◽  
Virus Like Particles

An examination of Penicillium notatum for the presence of Penicillium chrysogenum-type virus particles

1972 ◽  
Vol 18 (8) ◽  
pp. 1352-1353 ◽  
Author(s):  
Olga Volkoff ◽  
Teresa Walters ◽  
Rosamund A. Dejardin
Keyword(s):  
Virus Infection ◽  
Penicillium Chrysogenum ◽  
Type Virus ◽  
Penicillium Notatum ◽  
Virus Like Particles ◽  
Virus Particles ◽  
Infected Cells

Virus-like particles have been shown to be present in all strains of Penicillium chrysogenum examined but not in penicillin producing strains of P. notatum. Attempts to "cure" P. chrysogenum of the virus infection were not successful, nor was it possible to infect P. notatum with the purified virus from P. chrysogenum. Examination of longitudinal sections of the mycelia of P. chrysogenum showed that the virus particles are enclosed in vesicles in the infected cells.

scholarly journals A spectrophotometric study of the secondary structure of ribonucleic acid isolated from the smaller and larger ribosomal subparticles of rabbit reticulocytes

1970 ◽  
Vol 117 (1) ◽  
pp. 101-118 ◽  
Author(s):  
R. A. Cox
Keyword(s):  
Escherichia Coli ◽  
Ionic Strength ◽  
Secondary Structure ◽  
Ribonucleic Acid ◽  
Spectrophotometric Study ◽  
Urea Concentration ◽  
Model Systems ◽  
Base Pairs ◽  
Virus Like Particles ◽  
Rabbit Reticulocytes

The spectrum of RNA from the smaller and larger subparticles of rabbit reticulocyte ribosomes was studied as a function of pH, ionic strength, urea concentration and temperature. It was inferred that both RNA species form short double-helical segments of not more than about 10 base-pairs in length. Not more than about 70% of the base residues may be located in double-helical segments. RNA from the larger subparticle is richer in guanine and cytosine residues and its secondary structure is the more stable. These conclusions are based on the use of double-helical RNA from virus-like particles and of unfractionated Escherichia coli tRNA as model systems.

On the mycophage of Penicillium chrysogenum

1973 ◽  
Vol 19 (1) ◽  
pp. 97-103 ◽  
Author(s):  
C. H. Nash ◽  
R. J. Douthart ◽  
L. F. Ellis ◽  
R. M. Van Frank ◽  
J. P. Burnett ◽  
...  
Keyword(s):  
Ribonucleic Acid ◽  
Penicillium Chrysogenum ◽  
Thermal Denaturation ◽  
Buoyant Density ◽  
Ion Concentration ◽  
Contour Length ◽  
Viral Dsrna ◽  
Viral Particles ◽  
Profile Characteristic ◽  
Isopycnic Centrifugation

A mycovirus has been purified from mycelia of Penicillium chrysogenum by isopycnic centrifugation in sucrose and in CsCl. Viral particles band with a buoyant density of 1.20 in sucrose and 1.38 in CsCl. Particles have icosohedral symmetry, are 35 nm in diameter, and have an absorption profile characteristic of nucleoprotein. One enzymatic activity, RNA polymerase, is associated with the purified mycophage. Nucleic acid extracted from purified virus has a buoyant density in CS2SO4 of 1.61, a molar extinction coefficient of εp (258 nm) of 7200, a s20, w of 13.0, and a pattern of circular dichroism characteristic of double-helical ribonucleic acid. Molecules of this double-stranded ribonucleic acid (dsRNA), examined by electron microscopy, have a mean contour length of 0.86 μm which corresponds to a molecular weight of about 2.0 × 106 daltons. This dsRNA is resolved further by acrylamide gel electrophoresis into three closely spaced bands. Thermal denaturation of the viral dsRNA is dependent on ionic strength and gives a linear relationship with the negative logarithm of the sodium ion concentration.

DETECTION OF VIRUS-LIKE PARTICLES IN THE COREMIA OFPENICILLIUM CLAVIFORME

1975 ◽  
Vol 17 (4) ◽  
pp. 525-533 ◽  
Author(s):  
H. C. Lai ◽  
K. Zachariah
Keyword(s):  
Ribonucleic Acid ◽  
Ecological Significance ◽  
Wild Type ◽  
Virus Like Particles ◽  
Mutant Strains ◽  
Fungal Viruses

A double-stranded ribonucleic acid (dsRNA) was isolated from coremial extracts of wild type P. claviforme, by methylated-albumin kieselguhr chromatography. Differential centrifugations of the coremial extracts from WT and Sh mutant strains yielded two classes of virus-like particles (VLP), of dimensions 25-30 nm, and 50-70 nm. The possible ecological significance of fungal viruses is discussed.

Virus-like Particles in Penicillin producing Strains of Penicillium chrysogenum

Nature ◽  
1969 ◽  
Vol 222 (5188) ◽  
pp. 89-90 ◽  
Author(s):  
G. T. BANKS ◽  
K. W. BUCK ◽  
E. B. CHAIN ◽  
JOAN E. DARBYSHIRE ◽  
F. HIMMELWEIT
Keyword(s):  
Penicillium Chrysogenum ◽  
Virus Like Particles
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