Denaturation and renaturation of Penicillium chrysogenum mycophage double-stranded ribonucleic acid in tetraalkylammonium salt solutions

Biochemistry ◽  
1981 ◽  
Vol 20 (11) ◽  
pp. 2999-3002 ◽  
Author(s):  
James G. Wetmur ◽  
William T. Ruyechan ◽  
Richard J. Douthart
Keyword(s):  
Ribonucleic Acid ◽  
Penicillium Chrysogenum ◽  
Tetraalkylammonium Salt ◽  
Salt Solutions

On the mycophage of Penicillium chrysogenum

1973 ◽  
Vol 19 (1) ◽  
pp. 97-103 ◽  
Author(s):  
C. H. Nash ◽  
R. J. Douthart ◽  
L. F. Ellis ◽  
R. M. Van Frank ◽  
J. P. Burnett ◽  
...  
Keyword(s):  
Ribonucleic Acid ◽  
Penicillium Chrysogenum ◽  
Thermal Denaturation ◽  
Buoyant Density ◽  
Ion Concentration ◽  
Contour Length ◽  
Viral Dsrna ◽  
Viral Particles ◽  
Profile Characteristic ◽  
Isopycnic Centrifugation

A mycovirus has been purified from mycelia of Penicillium chrysogenum by isopycnic centrifugation in sucrose and in CsCl. Viral particles band with a buoyant density of 1.20 in sucrose and 1.38 in CsCl. Particles have icosohedral symmetry, are 35 nm in diameter, and have an absorption profile characteristic of nucleoprotein. One enzymatic activity, RNA polymerase, is associated with the purified mycophage. Nucleic acid extracted from purified virus has a buoyant density in CS2SO4 of 1.61, a molar extinction coefficient of εp (258 nm) of 7200, a s20, w of 13.0, and a pattern of circular dichroism characteristic of double-helical ribonucleic acid. Molecules of this double-stranded ribonucleic acid (dsRNA), examined by electron microscopy, have a mean contour length of 0.86 μm which corresponds to a molecular weight of about 2.0 × 106 daltons. This dsRNA is resolved further by acrylamide gel electrophoresis into three closely spaced bands. Thermal denaturation of the viral dsRNA is dependent on ionic strength and gives a linear relationship with the negative logarithm of the sodium ion concentration.

scholarly journals Purification of Potato Virus X and Preparation of Infectious Ribonucleic Acid by Degradation With Lithium Chloride

1968 ◽  
Vol 21 (6) ◽  
pp. 1311 ◽  
Author(s):  
RIB Francki ◽  
GD Mclean
Keyword(s):  
Lithium Chloride ◽  
Potato Virus ◽  
Ribonucleic Acid ◽  
Potato Virus X ◽  
Plant Viruses ◽  
Salt Solutions

Potato virus X (PVX) was one of the first plant viruses to be partially purified (Bawden and Pirie 1938). Purification by precipitation with salt solutions, however, resulted in aggregation of the particles. which became entwined to form extensive rope and netlike structures (Kleczkowski and Nixon 1950). Markham (1959) suggested that the problem of aggregation and the formation of gels when the virus is treated with salt solutions has prevented PYX from being extensively studied

scholarly journals The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones

1988 ◽  
Vol 16 (10) ◽  
pp. 4637-4650 ◽  
Author(s):  
Kenneth A. Jacobs ◽  
Richard Rudersdorf ◽  
Suzanne D. Neill ◽  
Joseph P. Dougherty ◽  
Eugene L. Brown ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Recombinant Dna ◽  
Tetraalkylammonium Salt ◽  
Salt Solutions ◽  
Thermal Stability Of

Salt-specific effects observed in calorimetric studies of alkali and tetraalkylammonium salt solutions of poly(thiophen-3-ylacetic acid)

2015 ◽  
Vol 17 (4) ◽  
pp. 2475-2483 ◽  
Author(s):  
Gregor Hostnik ◽  
Vojko Vlachy ◽  
Dmitrij Bondarev ◽  
Jir̆í Vohlídal ◽  
Janez Cerar
Keyword(s):  
Tetraalkylammonium Salt ◽  
Salt Solutions ◽  
Specific Effects ◽  
Calorimetric Studies ◽  
Ylacetic Acid ◽  
Acid Salts

Differences in hydration of counterions are blamed for strong salt-specific effects produced upon dilution and mixing of poly(thiophene-3-ylacetic acid) salts with simple salts.

scholarly journals Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum

1970 ◽  
Vol 120 (3) ◽  
pp. 549-558 ◽  
Author(s):  
R. A. Cox ◽  
K. Kanagalingam ◽  
Elisabeth S. Sutherland
Keyword(s):  
Ribonucleic Acid ◽  
Penicillium Chrysogenum ◽  
Virus Like Particles
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