scholarly journals The composition of triglycerides from liver, egg yolk and adipose tissue of the laying hen

1970 ◽  
Vol 120 (2) ◽  
pp. 365-371 ◽  
Author(s):  
D. R. Husbands
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Thin Layer ◽  
Pancreatic Lipase ◽  
Egg Yolk ◽  
Standard Diet ◽  
Argentation Thin Layer Chromatography ◽  
Degree Of Unsaturation ◽  
Liver Triglycerides ◽  
Layer Chromatography

The composition of the triglycerides of liver, egg yolk and adipose tissue of laying hens fed on a standard diet were investigated by using argentation thin-layer chromatography to separate the triglycerides according to their degree of unsaturation. About 40% of liver triglycerides consisted of one saturated and two monoenoic fatty acids. Triglycerides containing linoleate were more abundant in adipose tissue than in either yolk or liver. Hydrolysis by pancreatic lipase of the tissue triglycerides and fractions obtained from these triglycerides showed that the triglycerides of adipose tissue had a less ordered arrangement of fatty acids at the 2-position than did either yolk or liver triglycerides. The labelling patterns of triglycerides formed in liver slices incubated in the presence of [1-(3)14C]glycerol indicated that triglycerides containing four or more double bonds are formed to a greater extent than are other triglyceride fractions. This is evidence for the concept that the type of triglyceride formed depends on the availability of fatty acids to the liver cells.

Enantiomeric Diglycerides as Stereospecific Probes in Triglyceride Synthesis In Vitro

1972 ◽  
Vol 50 (8) ◽  
pp. 881-887 ◽  
Author(s):  
P. J. A. O'Doherty ◽  
A. Kuksis ◽  
D. Buchnea
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Positional Distribution ◽  
Argentation Thin Layer Chromatography ◽  
Glycerol Molecule ◽  
Direct Acylation ◽  
Triglyceride Biosynthesis ◽  
Layer Chromatography ◽  
Single Enzyme

The stereospecificity of diglyceride acyltransferase (EC 2.3.1.20) was studied in the microsomes of rat intestine and liver using enantiomeric diglycerides as acceptors of labeled fatty acids. The diglyceride mixtures were prepared by combining equal amounts of synthetic 1,2- and 2,3-diglycerides of different degrees of unsaturation. The labeled triglycerides formed were resolved by argentation thin-layer chromatography, whereupon the products from each enantiomeric diglyceride moved to a different spot on the thin-layer plate.It was shown that both 1,2- and 2,3-diglycerides were utilized for direct acylation to triglycerides by both tissues. The diglyceride acyltransferase, if a single enzyme, showed a definite preference for the acylation of the 1,2-diglycerides. The intestine esterified more of the 2,3-isomer than did the liver. The yields of triglyceride depended upon the nature of the fatty acids involved, and their positional distribution on the glycerol molecule. The new method of stereospecific assessment of triglyceride biosynthesis in vitro is applicable to preparations of other subcellular fractions and to other suitable combinations of fatty acid and diglyceride precursors.

THE ISOLATION OF BEEF SPHINGOMYELINS

1964 ◽  
Vol 42 (12) ◽  
pp. 1659-1675 ◽  
Author(s):  
W. A. Spencer ◽  
R. Schaffrin
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Infrared Spectroscopy ◽  
Thin Layer ◽  
Water Soluble ◽  
Beef Heart ◽  
Selective Hydrolysis ◽  
Solvent Fractionation ◽  
Hydrolysis Products ◽  
Layer Chromatography

A method was developed for the quantitative isolation of tissue sphingomyelins. Total lipid extracts of tissues were subjected to selective hydrolysis to destroy ester phosphatides and plasmalogens. The water-soluble hydrolysis products were removed by solvent fractionation. The sphingomyelins were separated from other lipids on silicic acid columns.Sphingomyelins were prepared from beef heart, lung, kidney, adrenal, brain, liver, and adipose tissue. The sphingomyelins were analyzed by thin-layer chromatography, paper chromatography of hydrolysis products, and infrared spectroscopy. Fatty acids were analyzed by gas–liquid chromatography.Methods for preparation and analysis of sphingomyelins are discussed.

Effect of Propolis-Supplemented Diet on Body Composition and Endocrine Function of Adipose Tissue

2020 ◽  
Vol 19 (2) ◽  
pp. 217-221
Author(s):  
Maria Jesús Lisbona-González ◽  
Candela Reyes-Botella ◽  
Esther Muñoz-Soto ◽  
Maria Victoria Olmedo-Gaya, ◽  
Jorge Moreno-Fernandez ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Composition ◽  
Adipose Tissue ◽  
Endocrine Function ◽  
Control Group ◽  
Albino Rats ◽  
Standard Diet ◽  
Reduced Body ◽  
Esterified Fatty Acids ◽  
Wistar Albino Rats

Adipose tissue is an endocrine organ and has central role in interaction with other organs or tissues while propolis can induce lipolysis. Therefore, the aim of this study is to provide detailed information about adipose tissue homeostasis modifications and body composition during propolis supplement consumption. Twenty male Wistar albino rats (8 weeks) were divided into two groups of 10 animals each and fed for 90 days with two different types of diets: standard for the control group (diet C) and standard diet + 2% propolis (diet P). Thyroid hormones did not show differences, while ghrelin and adiponectin decreased in the group that was fed propolis. Insulin, leptin, and non-esterified fatty acids also increased along with reduced body weight and fat, in addition to increased lean mass when propolis was in the diet. We conclude that propolis could decrease ghrelin and adiponectin but increase non-esterified fatty acids and insulin secretion, which improves body composition.

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