scholarly journals The chemical synthesis of 1-O-(indol-3′-ylacetyl)-β-d-glucopyranose. The higher activity of the glucoside in comparison with exogenous indol-3-ylacetic acid in plant-section elongation tests

1969 ◽  
Vol 114 (4) ◽  
pp. 827-832 ◽  
Author(s):  
D. Keglević ◽  
M. Pokorny

1. The synthesis of 1-O-(indol-3′-ylacetyl)-β-d-glucopyranose via the fully benzylated 1-O-(indol-3′-ylacetyl)-d-glucopyranose is described. The configuration of the free ester glucoside was confirmed by complete hydrolysis with β-glucosidase and by the n.m.r. spectrum of the tetra-acetyl derivative. 2. The growth-promoting effect of the glucoside in Avena coleoptile- and pea stem-section tests distinctly exceeds the responses stimulated by equimolar amounts of indol-3-ylacetic acid or equimolar mixtures of indol-3-ylacetic acid and glucose at all concentrations investigated. Time-sequence experiments revealed that the sections stimulated by the glucoside exhibit a markedly greater rate of elongation than those promoted by indol-3-ylacetic acid. 3. 1-O-(Indol-3′-ylacetyl)-β-d-glucopyranose was isolated from intact Avena coleoptiles. 4. According to the results, the conjugation of indol-3-ylacetic acid with glucose could not be considered merely as a detoxication mechanism for indol-3-ylacetic acid in plant tissues.

1990 ◽  
Vol 80 (1) ◽  
pp. 109-113 ◽  
Author(s):  
Ester P. Lorences ◽  
Gordon J. McDougall ◽  
Stephen C. Fry

Zygote ◽  
2008 ◽  
Vol 16 (3) ◽  
pp. 239-247 ◽  
Author(s):  
T. Metoki ◽  
H. Iwata ◽  
M. Itoh ◽  
M. Kasai ◽  
A. Takajyo ◽  
...  

SummaryWe examined the effect of supplementing the culture medium with follicular fluid (FF) on the growth of porcine preantral follicles and oocytes. Firstly, preantral follicles were retrieved from ovaries and then FF was collected from all antral follicles that were 2–7 mm in diameter (AFF), which included large follicles of 4–7 mm in diameter (LFF) and small follicles of 2–3 mm in diameter (SFF). When preantral follicles with a diameter of 250 μm were cultured in medium containing AFF, the growth of follicles and oocytes was greater than when follicles were cultured in medium containing fetal calf serum (FCS). When this growth-promoting effect in AFF was compared for LFF and SFF, the LFF were shown to be significantly more effective than SFF. This LFF effect was lost, however, when the concentration of LFF in the medium was decreased from 5% to 0.5% or when LFF were heat treated (60 °C for 30 min) or trypsin was added. In contrast, a decrease in SFF concentration from 5% to 0.5% and heat treatment of the SFF enhanced preantral follicle growth. Furthermore, proteins obtained from LFF that had molecular weights greater than 10 kDa (LFF > 10 kDa) had similar, but relatively reduced, growth-promoting properties. The remaining three LFF protein fractions (<10 kDa or <100 kDa or >100 kDa), however, did not have these growth-promoting properties. In conclusion, the supplementation of medium with LFF, rather than serum, enhanced preantral follicle and oocyte growth. Factors that enhanced follicle development in LFF and factors that suppressed follicle development in SFF were proteins and these LFF factors ranged in size from 10 kDa to over 100 kDa.


The Analyst ◽  
2021 ◽  
Author(s):  
Yuchen Zhang ◽  
Rachel Komorek ◽  
Jiyoung Son ◽  
Shawn Riechers ◽  
Zihua Zhu ◽  
...  

Plant growth-promoting rhizobacteria (PGPR) play a crucial role in biological control and pathogenic defense on and within plant tissues, however the mechanism(s) by which plants associate with PGPR to elicit...


2000 ◽  
Vol 118 (4) ◽  
pp. A602
Author(s):  
Shigeo Takaishi ◽  
Mitsutaka Sawada ◽  
Yukari Morita ◽  
Hiroaki Fukuzawa ◽  
Hiroshi Seno ◽  
...  

1997 ◽  
Vol 43 (4) ◽  
pp. 225-230 ◽  
Author(s):  
Michio Matsuhashi ◽  
Katsura Endoh ◽  
Alla N. Pankrushina ◽  
Hiroshi Watanabe ◽  
Mayumi Komiyama ◽  
...  

1962 ◽  
Vol 37 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Carol Steinhart ◽  
Laurens Anderson ◽  
Folke Skoog

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