scholarly journals Determination of amino sugars in mixtures containing glucosamine, galactosamine and muramic acid

1968 ◽  
Vol 109 (1) ◽  
pp. 13-18 ◽  
Author(s):  
D. E. S. Stewart-Tull
Keyword(s):  
Cell Wall ◽  
Quantitative Determination ◽  
Column Chromatography ◽  
Bacterial Species ◽  
Colorimetric Method ◽  
Previous Treatment ◽  
Amino Sugars ◽  
Muramic Acid ◽  
Molar Ratios

A colorimetric method is described whereby the direct quantitative determination of glucosamine, galactosamine and muramic acid can be achieved without previous treatment of the cell-wall hydrolysate, for example by column chromatography. Molar ratios of hexosamines in cell-wall preparations, from a number of bacterial species, determined by this method were found to be in general agreement with previously published results.

Enhanced peroxidase-like activity of Mo-doped ceria nanoparticles for sensitive colorimetric detection of glucose

2018 ◽  
Vol 10 (1) ◽  
pp. 76-83 ◽  
Author(s):  
Xue Jiao ◽  
Wanyi Liu ◽  
Di Wu ◽  
Wenhao Liu ◽  
Hongjie Song
Keyword(s):  
Catalytic Activity ◽  
Blood Glucose ◽  
Quantitative Determination ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Ceria Nanoparticles ◽  
Doped Ceria ◽  
Enzyme Mimics ◽  
Small Nanoparticles

Ultra-small nanoparticles of Mo-doped ceria (Mo/CeO2 NPs) possess enhanced peroxidase-like catalytic activity and these enzyme mimics are used here for the successful quantitative determination of blood glucose via a colorimetric method.

Colorimetric detection of hypochlorite based on the morphological changes of silver nanoprisms to spherical nanoparticles

2017 ◽  
Vol 9 (21) ◽  
pp. 3151-3158 ◽  
Author(s):  
Thangarasu Sasikumar ◽  
Malaichamy Ilanchelian
Keyword(s):  
Quantitative Determination ◽  
Morphological Changes ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Spherical Nanoparticles ◽  
Colorimetric Probe ◽  
Silver Nanoprisms

In this work, we have developed a simple, rapid, sensitive and selective colorimetric method for the quantitative determination of hypochlorite (ClO−) ions by using triangular silver nanoprisms (AgNPRs) as a colorimetric probe.

Collaborative Study of a Method for Sodium Lauryl Sulfate in Egg Whites

1968 ◽  
Vol 51 (3) ◽  
pp. 540-543
Author(s):  
John Wiskerchen
Keyword(s):  
Sulfuric Acid ◽  
Quantitative Determination ◽  
Sodium Lauryl Sulfate ◽  
Collaborative Study ◽  
Colorimetric Method ◽  
Egg White ◽  
Mean Deviation ◽  
Lauryl Sulfate ◽  
Benzethonium Chloride

Abstract A colorimetric method for the quantitative determination of sodium lauryl sulfate in liquid, frozen, and powdered egg white was studied by eight collaborators. Determinations were made on flake and powdered egg white at levels of 0.05, 0.1, and 0.2% (w/w) and on liquid egg white at levels of 0.006, 0.0125, and 0.0250% (w/w) sodium lauryl sulfate. The egg white is dissolved in water, and the protein is precipitated with ethanol and removed by filtration. An aliquot of the filtrate is evaporated to dryness, and the residue is dissolved in water and acidified with sulfuric acid. The sodium lauryl sulfate is complexed with Azure A, extracted into chloroform, and determined spectrophotometrically at 637 mμ. A blank determination is made on another aliquot of the filtrate by complexing the sodium lauryl sulfate with benzethonium chloride. This is a stable colorless complex. Average recoveries in the collaborative study were 98—102% with a mean deviation of 2.8—5.4%. It is recommended that the method be adopted as official, first action.

A colorimetric method for the quantitative determination of thiazolidine-4-carboxylic acids

1966 ◽  
Vol 17 (3) ◽  
pp. 513-520 ◽  
Author(s):  
Guido G. Guidotti ◽  
Angelo F. Borghetti ◽  
Lodovico Loreti
Keyword(s):  
Quantitative Determination ◽  
Carboxylic Acids ◽  
Colorimetric Method

Colorimetric Method for the Quantitative Determination of Acetaminophen in Serum

1979 ◽  
Vol 15 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Christopher S. Frings ◽  
Joseph M. Saloom
Keyword(s):  
Quantitative Determination ◽  
Colorimetric Method

ROUTINE DETERMINATIONS OF 17-KETOSTEROIDS AND PORTER-SILBER CHROMOGENS COMPARED WITH THE CHROMATOGRAPHIC MEASUREMENT OF GROUPED AND INDIVIDUAL STEROIDS

1964 ◽  
Vol 46 (4) ◽  
pp. 552-562 ◽  
Author(s):  
Ingrid Ernest ◽  
Britt Håkansson ◽  
Jörgen Lehmann ◽  
Björn Sjögren
Keyword(s):  
Urinary Excretion ◽  
Quantitative Determination ◽  
Column Chromatography ◽  
Chromatographic Separation ◽  
Enzyme Hydrolysis ◽  
Routine Method ◽  
Urinary Steroids ◽  
Urinary Content ◽  
Chromatographic Measurement

ABSTRACT The accuracy of two routine methods for the determination of urinary steroids – 17-ketosteroids (17-KS) and Porter-Silber chromogens – has been investigated by chromatographic separation and quantitative determination of individual 17-KS and Porter-Silber reacting steroids in 141 urine samples. For this purpose urine was submitted to enzyme hydrolysis and subsequent solvolysis. The pertinent steroids were separated by column chromatography into three groups, 11-deoxy-17-KS, 11-oxy-17-KS and Porter-Silber reacting steroids. The final separation was accomplished by paper chromatography. As a mean, the urinary excretion of true 17-KS corresponded to about 40–50 per cent of the routine figures. Due to non specific chromogens, individual routine figures were completely unreliable and probably had no more significance than showing a difference between a high and a low urinary content of 17-KS A true figure, however, was never higher than that indicated by the routine method. The routine method for determination of Porter-Silber chromogens also overestimated the urinary content of steroids, the true excretion of Porter-Silber steroids being, on an average, about 25 % lower. Again, the significance of individual determinations was low. The determination of 17-KS and Porter-Silber steroids by column chromatography was found to be rather simple and reliable as only minor amounts of unspecific chromogens were included in the results. Moreover with this method, the 17-KS were separated into 11-deoxy-17-KS and 11-oxy-17-KS.

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