Combined application of column chromatography and spectrofluorometry for the quantitative determination of individual cortisole and corticosterone in the blood and urine

1970 ◽  
Vol 19 (6) ◽  
pp. 1313-1315
Author(s):  
L. D. Litvinov ◽  
L. A. Sadovskaya
Keyword(s):  
Quantitative Determination ◽  
Column Chromatography ◽  
Combined Application

scholarly journals Determination of amino sugars in mixtures containing glucosamine, galactosamine and muramic acid

1968 ◽  
Vol 109 (1) ◽  
pp. 13-18 ◽  
Author(s):  
D. E. S. Stewart-Tull
Keyword(s):  
Cell Wall ◽  
Quantitative Determination ◽  
Column Chromatography ◽  
Bacterial Species ◽  
Colorimetric Method ◽  
Previous Treatment ◽  
Amino Sugars ◽  
Muramic Acid ◽  
Molar Ratios

A colorimetric method is described whereby the direct quantitative determination of glucosamine, galactosamine and muramic acid can be achieved without previous treatment of the cell-wall hydrolysate, for example by column chromatography. Molar ratios of hexosamines in cell-wall preparations, from a number of bacterial species, determined by this method were found to be in general agreement with previously published results.

ROUTINE DETERMINATIONS OF 17-KETOSTEROIDS AND PORTER-SILBER CHROMOGENS COMPARED WITH THE CHROMATOGRAPHIC MEASUREMENT OF GROUPED AND INDIVIDUAL STEROIDS

1964 ◽  
Vol 46 (4) ◽  
pp. 552-562 ◽  
Author(s):  
Ingrid Ernest ◽  
Britt Håkansson ◽  
Jörgen Lehmann ◽  
Björn Sjögren
Keyword(s):  
Urinary Excretion ◽  
Quantitative Determination ◽  
Column Chromatography ◽  
Chromatographic Separation ◽  
Enzyme Hydrolysis ◽  
Routine Method ◽  
Urinary Steroids ◽  
Urinary Content ◽  
Chromatographic Measurement

ABSTRACT The accuracy of two routine methods for the determination of urinary steroids – 17-ketosteroids (17-KS) and Porter-Silber chromogens – has been investigated by chromatographic separation and quantitative determination of individual 17-KS and Porter-Silber reacting steroids in 141 urine samples. For this purpose urine was submitted to enzyme hydrolysis and subsequent solvolysis. The pertinent steroids were separated by column chromatography into three groups, 11-deoxy-17-KS, 11-oxy-17-KS and Porter-Silber reacting steroids. The final separation was accomplished by paper chromatography. As a mean, the urinary excretion of true 17-KS corresponded to about 40–50 per cent of the routine figures. Due to non specific chromogens, individual routine figures were completely unreliable and probably had no more significance than showing a difference between a high and a low urinary content of 17-KS A true figure, however, was never higher than that indicated by the routine method. The routine method for determination of Porter-Silber chromogens also overestimated the urinary content of steroids, the true excretion of Porter-Silber steroids being, on an average, about 25 % lower. Again, the significance of individual determinations was low. The determination of 17-KS and Porter-Silber steroids by column chromatography was found to be rather simple and reliable as only minor amounts of unspecific chromogens were included in the results. Moreover with this method, the 17-KS were separated into 11-deoxy-17-KS and 11-oxy-17-KS.

Quantitative determination of γ-globulin in the human sera by column chromatography

1968 ◽  
Vol 22 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Elizabeth Roboz Einstein ◽  
Karen Richard ◽  
Prisca Cerutti
Keyword(s):  
Quantitative Determination ◽  
Column Chromatography ◽  
Human Sera

Quantitative determination of glucosamine by column chromatography and radioisotope dilution

1971 ◽  
Vol 39 (1) ◽  
pp. 251-257 ◽  
Author(s):  
Philip C. Kelleher ◽  
Charles B. Howard ◽  
Carol J. Smith
Keyword(s):  
Quantitative Determination ◽  
Column Chromatography

The Determination of Mono-, Di-, and Triglycerides in Monoglyceride Concentrates by Column Chromatography

1965 ◽  
Vol 48 (2) ◽  
pp. 444-448
Author(s):  
E Distler ◽  
F J Baur
Keyword(s):  
Standard Deviation ◽  
Quantitative Determination ◽  
Silica Gel ◽  
Column Chromatography ◽  
Ethyl Ether ◽  
Average Standard Deviation

Abstract A method for the quantitative determination of mono-, di-, and triglycerides was studied collaboratively. Samples are adsorbed on silica gel columns and the glycerides are separated by benzene, benzene plus 10% ethyl ether, and ethyl ether alone. Eight samples can be run simultaneously with an average standard deviation of 0.6–1.0%. Nine collaborators obtained average recoveries of 96, 100.3, and 102% for mono-, di-, and triglycerides, respectively.

Emulsifiers. I. Determination of Mono-, Di-, and Triglycerides in Shortenings by Column Chromatography

1966 ◽  
Vol 49 (4) ◽  
pp. 812-816
Author(s):  
E Distler ◽  
F J Baur
Keyword(s):  
Standard Deviation ◽  
Quantitative Determination ◽  
Silica Gel ◽  
Column Chromatography ◽  
Ethyl Ether ◽  
Average Standard Deviation

Abstract A method for the quantitative determination of mono-, di-, and triglycerides in shortenings was studied collaboratively. Samples were adsorbed on silica gel columns and the glycerides were separated by benzene, benzene plus 10% ethyl ether, and ethyl ether alone. Eight samples can be run simultaneously with an average standard deviation of 0.1–0.5%. This method is recommended for adoption as official, first action.

Sign in / Sign up

Export Citation Format

Share Document