scholarly journals The effect of γ-irradiation on soluble collagen

1968 ◽  
Vol 107 (1) ◽  
pp. 29-34 ◽  
Author(s):  
R. J. Davidson ◽  
D. R. Cooper
Keyword(s):  
Conformational Changes ◽  
Irradiation Dose ◽  
Neutral Salt ◽  
Native Structure ◽  
Γ Irradiation ◽  
Peptide Bonds ◽  
Soluble Collagen ◽  
Acid Soluble Collagen ◽  
Principal Effect ◽  
Initial Effect

1. A study was made of the effect of γ-irradiation on the sub-unit composition, as well as the conformational changes taking place in cooled solutions of thermally denatured neutral-salt-soluble and acid-soluble collagen. 2. The increase in negative rotation and viscosity at 15° for irradiated and thermally denatured collagen solutions becomes less as the irradiation dose is increased. 3. The initial effect of γ-irradiation is the depolymerization of the dimers found in both neutral-salt-soluble and acid-soluble collagen. 4. The principal effect of γ-irradiation up to 10 Mrads is the fission of peptide bonds, yielding crystalline irradiation-resistant portions of the molecule incapable of associating to the native structure. 5. The effects of γ-irradiation on both neutral-salt-soluble and acid-soluble collagen are very similar and bear a close resemblance to the effects induced by ultraviolet irradiation.

scholarly journals The effect of ultraviolet irradiation on acid-soluble collagen

1967 ◽  
Vol 105 (3) ◽  
pp. 965-969 ◽  
Author(s):  
R. J. Davidson ◽  
D. R. Cooper
Keyword(s):  
Conformational Changes ◽  
Ultraviolet Irradiation ◽  
Irradiation Dose ◽  
Helical Structure ◽  
Neutral Salt ◽  
Soluble Collagen ◽  
Acid Soluble Collagen ◽  
Skin Collagen ◽  
Principal Effect ◽  
Chain Lengths

1. A study has been made of the effect of ultraviolet irradiation on the conformational changes taking place in cooled solutions of thermally denatured acid-soluble calf-skin collagen. 2. The increase in negative rotation and viscosity at 15° for irradiated and thermally denatured collagen solutions becomes less as the irradiation dose is increased. 3. The principal effect of ultraviolet irradiation is the fission of the primary collagen chains, eventually yielding chain lengths incapable of stabilizing a helical structure. 4. The effects of ultraviolet irradiation on acid-soluble collagen may be closely correlated with similar effects on neutral salt-soluble collagen.

scholarly journals The decomposition of soluble collagen by γ-irradiation

1969 ◽  
Vol 113 (2) ◽  
pp. 263-269 ◽  
Author(s):  
D. R. Cooper ◽  
A. E. Russell
Keyword(s):  
Irradiation Dose ◽  
Optical Rotation ◽  
Specific Rotation ◽  
Γ Irradiation ◽  
Soluble Collagen ◽  
Heating And Cooling ◽  
Reduced Viscosity ◽  
Acid Soluble Collagen ◽  
Freeze Dried ◽  
High Degree

1. The effect of γ-irradiation in the range 1 krad–10 Mrads on freeze-dried acid-soluble collagen was studied. 2. The specific-rotation and reduced-viscosity recoveries after heating and cooling of the irradiated collagen in solution showed a high degree of dependence on irradiation dose, with reduced viscosity showing significantly less recovery than specific rotation on increasing the irradiation dose. 3. The dependence of reduced viscosity on concentration was greatly decreased with increased doses of γ-irradiation. 4. The melting temperature measured by optical rotation also decreased as the irradiation dose was increased, and at low doses was distinctly biphasic. 5. Physical properties showed that the action of γ-irradiation up to 10 Mrads occurred in two distinct phases, with the early changes being extremely sensitive to irradiation dose. 6. The action of the γ-irradiation is discussed in terms of the structure of tropocollagen.

Intermolecular Relationship between Neutral-salt-soluble and Acid-soluble Collagen

Nature ◽  
1968 ◽  
Vol 217 (5124) ◽  
pp. 168-169 ◽  
Author(s):  
R. J. DAVIDSON ◽  
D. R. COOPER
Keyword(s):  
Neutral Salt ◽  
Soluble Collagen ◽  
Acid Soluble Collagen

scholarly journals The effect of ultraviolet irradiation on soluble collagen

1965 ◽  
Vol 97 (1) ◽  
pp. 139-147 ◽  
Author(s):  
DR Cooper ◽  
RJ Davidson
Keyword(s):  
Ultraviolet Irradiation ◽  
Optical Rotation ◽  
Gel Filtration ◽  
Collagen Molecule ◽  
Neutral Salt ◽  
Soluble Collagen ◽  
Acid Soluble Collagen ◽  
Skin Collagen ◽  
Kinetics Of ◽  
Calf Skin

1. The effect of ultraviolet irradiation on acid-soluble and neutral-salt-soluble calf-skin collagen was studied by chromatography, gel filtration, amino acid analysis and sedimentation of the sub-units, and the reaction kinetics of degradation were obtained from viscosity and optical rotation measurements. 2. It was demonstrated that, whereas the structure of neutral-salt-soluble calf-skin collagen may be represented by the formula (alpha(1))(2)alpha(2), the acid-soluble extract has the formula alpha(1).(alpha(2))(2). The acid-soluble collagen is also unusual in containing a large amount of a component that could be beta(22). 3. Ultraviolet irradiation causes the progressive degradation of the collagen molecule into smaller molecular fragments that subsequently lose their helical nature. The rate constants show that the denaturation of soluble collagens by ultraviolet irradiation is much slower, under the conditions used, than denaturation by heat or enzymes.

scholarly journals Some properties of neutral-salt-soluble collagen. 1

1960 ◽  
Vol 76 (3) ◽  
pp. 452-463 ◽  
Author(s):  
J. H. Fessler
Keyword(s):  
Neutral Salt ◽  
Soluble Collagen

scholarly journals Chemical and Physical Properties of Cassava Starch–CM-Chitosan–Acrylic Acid Gel Copolymerization by Gamma Irradiation

2014 ◽  
Vol 14 (1) ◽  
pp. 37-42
Author(s):  
Gatot Trimulyadi Rekso
Keyword(s):  
Acrylic Acid ◽  
Irradiation Dose ◽  
Thermo Gravimetric Analysis ◽  
Gravimetric Analysis ◽  
Γ Irradiation ◽  
Equilibrium Degree ◽  
Preparation Conditions ◽  
Gel Fraction ◽  
Gelatinized Starch ◽  
Acid Gel

Starch is a renewable natural polymer that can be decomposed easily in the environment and can be modified to various applications such as biomedical, agricultural and pharmaceutical applications. Copolymerization of gelatinized starch–CM-chitosan and acrylic acid (AAc) in aqueous medium using γ-irradiation was carried out. The preparation conditions, such as irradiation dose and AAc concentration were investigated. The copolymers were characterized by FTIR spectroscopy, thermo-gravimetric analysis (TGA) and scanning electron microscopy (SEM). The results show that by increasing of irradiation dose the gel fraction increases till the dose of 15 kGy. Above the stating dose the gel fraction decreases. The Equilibrium Degree of Swelling (EDS) value slightly increases with increasing irradiation dose and after dose of 15 kGy is decreasing. The swelling of starch–CM-chitosan–AAc hydro gels reduced as the gel content increases. The results indicated that the optimum condition for obtaining hydro gels with desirable properties was irradiated at dose of 15 kGy. The results indicated that SEM revealed that the higher the dose, the lower the copolymer pore size. The starch–CM-chitosan–AAc copolymers have thermal stability higher than that for starch individually.

scholarly journals The Characterization of Acid Soluble Collagen from Sheep Tail Tendon

2021 ◽  
Author(s):  
Budjav Jadamba ◽  
Enerelt Urnukhsaikhan ◽  
Anujin Gantulga ◽  
Sugar Lkhagvachuluun ◽  
Enkhsaikhan Lkhagvasuren ◽  
...  
Keyword(s):  
Soluble Collagen ◽  
Acid Soluble Collagen ◽  
Tail Tendon
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