scholarly journals Developmental changes in alcohol-dehydrogenase activity in rat and guinea-pig liver

1967 ◽  
Vol 103 (3) ◽  
pp. 623-626 ◽  
Author(s):  
NCR Räihä ◽  
M Koskinen ◽  
P Pikkarainen
Keyword(s):  
Alcohol Dehydrogenase ◽  
Guinea Pig ◽  
Dehydrogenase Activity ◽  
Developmental Changes ◽  
Pig Liver ◽  
Alcohol Dehydrogenase Activity ◽  
Guinea Pig Liver

Elevation of Alcohol Dehydrogenase Activity in the Subclinically Scorbutic Guinea-Pig

1972 ◽  
Vol 42 (6) ◽  
pp. 781-784 ◽  
Author(s):  
Maureen O'keane ◽  
M. R. Moore ◽  
A. Goldberg
Keyword(s):  
Alcohol Dehydrogenase ◽  
Guinea Pig ◽  
Dehydrogenase Activity ◽  
Guinea Pigs ◽  
Liver Protein ◽  
Alcohol Dehydrogenase Activity ◽  
Nadh Ratio

1. Because it has been shown that a majority of alcoholics are subclinically scorbutic, the metabolism of ethanol was studied in subclinically-scorbutic guinea-pigs. 2. Hepatic alcohol dehydrogenase activity was raised maximally by ethanol within 2 days. 3. In twenty-three subclinically-scorbutic guinea-pigs fed ethanol for 2 weeks, the alcohol dehydrogenase activity (±SD) was 11·5 ± 1·2 units/g of liver protein compared with 8·6 ± 0·6 units/g of liver protein in twenty-three healthy animals fed ethanol. 4. The NAD+/NADH ratio in subclinically-scorbutic guinea-pigs and healthy guinea-pigs fed ethanol, shows that there is more NAD+ available for oxidation of alcohol in subclinically-scorbutic guinea-pigs. These results may explain the increased tolerance of alcoholics to alcohol.

Developmental Changes in UDP-N-Acetylglucosamine 2-Epimerase Activity of Rat and Guinea-Pig Liver

1997 ◽  
Vol 118 (1) ◽  
pp. 13-15 ◽  
Author(s):  
Beatriz Gal ◽  
Marı́a-José Ruano ◽  
Ricardo Puente ◽  
Luis A Garcı́a-Pardo ◽  
Ricardo Rueda ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Developmental Changes ◽  
Pig Liver ◽  
Guinea Pig Liver

scholarly journals Guinea-pig liver testosterone 17 β-dehydrogenase (NADP+) and aldehyde reductase exhibit benzene dihydrodiol dehydrogenase activity

1985 ◽  
Vol 225 (1) ◽  
pp. 177-181 ◽  
Author(s):  
A Hara ◽  
M Hayashibara ◽  
T Nakayama ◽  
K Hasebe ◽  
S Usui ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Dehydrogenase Activity ◽  
Aldehyde Reductase ◽  
Pig Liver ◽  
Liver Cytosol ◽  
Dihydrodiol Dehydrogenase ◽  
Guinea Pig Liver ◽  
Oxidation Of Benzene

We have kinetically and immunologically demonstrated that testosterone 17 beta-dehydrogenase (NADP+) isoenzymes (EC 1.1.1.64) and aldehyde reductase (EC 1.1.1.2) from guinea-pig liver catalyse the oxidation of benzene dihydrodiol (trans-1,2-dihydroxycyclohexa-3,5-diene) to catechol. One isoenzyme of testosterone 17 beta-dehydrogenase, which has specificity for 5 beta-androstanes, oxidized benzene dihydrodiol at a 3-fold higher rate than 5 beta-dihydrotestosterone, and showed a more than 4-fold higher affinity for benzene dihydrodiol and Vmax. value than did another isoenzyme, which exhibits specificity for 5 alpha-androstanes, and aldehyde reductase. Immunoprecipitation of guinea-pig liver cytosol with antisera against the testosterone 17 beta-dehydrogenase isoenzymes and aldehyde reductase indicated that most of the benzene dihydrodiol dehydrogenase activity in the tissue is due to testosterone 17 beta-dehydrogenase.

ALCOHOL DEHYDROGENASE ACTIVITY IN ISOLATED VACUOLES OF RED BEET ROOT CELLS

Tsitologiya ◽  
2018 ◽  
Vol 60 (6) ◽  
pp. 469-475
Author(s):  
O. D. Nimaeva ◽  
◽  
E. V. Pradedova ◽  
A. B. Karpova ◽  
R. K. Salyaev ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Root Cells ◽  
Beet Root ◽  
Red Beet ◽  
Alcohol Dehydrogenase Activity

Die Bedeutung der Homogenisationsart und -intensität für die Größe und Konstanz der Sauerstoffaufnahme von Meerschweinchen-Leberhomogenat / The Influence of Mode and Intensity of Homogenization on the Absolute Value and Stability of Oxygen Consumption of Guinea Pig Liver Homogenates

1977 ◽  
Vol 32 (11-12) ◽  
pp. 908-912 ◽  
Author(s):  
H. J. Schmidt ◽  
U. Schaum ◽  
J. P. Pichotka
Keyword(s):  
Oxygen Uptake ◽  
Guinea Pig ◽  
Measuring Time ◽  
Pig Liver ◽  
Absolute Value ◽  
Modified Method ◽  
Simultaneous Measurements ◽  
Liver Homogenates ◽  
The Absolute ◽  
Guinea Pig Liver

Abstract The influence of five different methods of homogenisation (1. The method according to Potter and Elvehjem, 2. A modification of this method called Potter S, 3. The method of Dounce, 4. Homogenisation by hypersonic waves and 5. Coarce-grained homogenisation with the “Mikro-fleischwolf”) on the absolute value and stability of oxygen uptake of guinea pig liver homogenates has been investigated in simultaneous measurements. All homogenates showed a characteristic fall of oxygen uptake during measuring time (3 hours). The modified method according to Potter and Elvehjem called Potter S showed reproducible results without any influence by homogenisation intensity.

Precision-cut Guinea-pig Liver Slices as a Tool for Studying the Toxicity of Volatile Anaesthetics

1990 ◽  
Vol 18 (1_part_1) ◽  
pp. 191-199
Author(s):  
Hanan N. Ghantous ◽  
Jeanne Fernando ◽  
Scott E. Morgan ◽  
A. Jay Gandolfi ◽  
Klaus Brandel
Keyword(s):  
Guinea Pig ◽  
Rank Order ◽  
Normal Liver ◽  
Liver Slice ◽  
Volatile Anaesthetics ◽  
Pig Liver ◽  
Liver Slices ◽  
Guinea Pig Liver ◽  
Krebs Henseleit Buffer

Cultured precision-cut liver slices retain normal liver architecture and physiological biochemical functions. Hartley male guinea-pig liver slices have proven to be a good model for studying the biotransformation and toxicity of halothane. This system was used to evaluate the biotransformation and toxicity of different volatile anaesthetics (halothane, enflurane, isoflurane and sevoflurane), and compare their effects to those of new anaesthetics (desflurane). Liver slices (250–300μm thick) were incubated in sealed roller vials, containing Krebs Henseleit buffer at 37°C under 95% O2:5% CO2 atmosphere. Volatile anaesthetics were delivered by volatilisation after pre-incubation for 1 hour to produce a constant concentration in the medium. Production of the metabolites, trifluroacetic acid and fluoride ion, was measured. Intracellular potassium ion content, protein synthesis and secretion were determined as indicators of viability of the slices. The rank order of biotransformation of anaesthetics by the liver slices was halothane >sevoflurane>isoflurane and enflurane>desflurane. The rank order of hepatotoxicity of these anaesthetics was halothane>isoflurane and enflurane>sevoflurane and desflurane. Halothane is the anaesthetic which is metabolised furthest and has the most toxic effect, while desflurane is the least metabolised anaesthetic and has the least toxicity. This in vitro cultured precision-cut liver slice system appears to be suitable for studying the biotransformation of volatile anaesthetics and correlating its role in the resulting toxicity.

scholarly journals Exolytic hydrolysis of toxic plant glucosides by guinea pig liver cytosolic beta-glucosidase.

1992 ◽  
Vol 267 (20) ◽  
pp. 14027-14032
Author(s):  
V Gopalan ◽  
A Pastuszyn ◽  
W R Galey ◽  
R.H. Glew
Keyword(s):  
Guinea Pig ◽  
Pig Liver ◽  
Beta Glucosidase ◽  
Toxic Plant ◽  
Hydrolysis Of ◽  
Guinea Pig Liver
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