Microquantitative determination of the distribution patterns of alcohol dehydrogenase activity in the liver of rat, guinea-pig and horse

1985 ◽  
Vol 83 (5) ◽  
pp. 431-436 ◽  
Author(s):  
I. P. Maly ◽  
D. Sasse
Keyword(s):  
Alcohol Dehydrogenase ◽  
Guinea Pig ◽  
Dehydrogenase Activity ◽  
Distribution Patterns ◽  
Alcohol Dehydrogenase Activity

Elevation of Alcohol Dehydrogenase Activity in the Subclinically Scorbutic Guinea-Pig

1972 ◽  
Vol 42 (6) ◽  
pp. 781-784 ◽  
Author(s):  
Maureen O'keane ◽  
M. R. Moore ◽  
A. Goldberg
Keyword(s):  
Alcohol Dehydrogenase ◽  
Guinea Pig ◽  
Dehydrogenase Activity ◽  
Guinea Pigs ◽  
Liver Protein ◽  
Alcohol Dehydrogenase Activity ◽  
Nadh Ratio

1. Because it has been shown that a majority of alcoholics are subclinically scorbutic, the metabolism of ethanol was studied in subclinically-scorbutic guinea-pigs. 2. Hepatic alcohol dehydrogenase activity was raised maximally by ethanol within 2 days. 3. In twenty-three subclinically-scorbutic guinea-pigs fed ethanol for 2 weeks, the alcohol dehydrogenase activity (±SD) was 11·5 ± 1·2 units/g of liver protein compared with 8·6 ± 0·6 units/g of liver protein in twenty-three healthy animals fed ethanol. 4. The NAD+/NADH ratio in subclinically-scorbutic guinea-pigs and healthy guinea-pigs fed ethanol, shows that there is more NAD+ available for oxidation of alcohol in subclinically-scorbutic guinea-pigs. These results may explain the increased tolerance of alcoholics to alcohol.

Improved assay for alcohol dehydrogenase activity in serum by centrifugal analysis.

1984 ◽  
Vol 30 (11) ◽  
pp. 1817-1820 ◽  
Author(s):  
S Kato ◽  
H Ishii ◽  
S Kano ◽  
S Hagihara ◽  
T Todoroki ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Clinical Utility ◽  
Final Concentration ◽  
Dilution Curve ◽  
Healthy Individuals ◽  
Improved Method ◽  
Simultaneous Reduction ◽  
Alcohol Dehydrogenase Activity

Abstract We describe an improved method for determination of alcohol dehydrogenase (EC 1.1.1.1) activity in 60 microL of human serum, based on conversion of ethanol to acetaldehyde with simultaneous reduction of NAD+ in glycine NaOH buffer (pH 9.0) at 37 degrees C in a centrifugal analyzer. The final concentration of NAD+ was 10 mmol/L and ethanol was 20 mmol/L. The dilution curve was linear with enzyme activity up to 200 U/L, and results by this method correlated well with those by a manual method (N Engl J Med 279: 241-248, 1968). Within-run precision (CV) was 0.9 to 8.2% over the range of 4.5 to 88.1 U/L, and day-to-day precision was 5.4 to 5.6%. In sera from 198 healthy individuals, mean alcohol dehydrogenase activity was 1.6 (SD 1.2, range 0-5) U/L. To evaluate the clinical utility of determining alcohol dehydrogenase, we measured the activity of alanine aminotransferase and alcohol dehydrogenase in sera from 470 patients with various diseases in our hospital, and found that results for the two enzymes did not correlate well.

ALCOHOL DEHYDROGENASE ACTIVITY IN ISOLATED VACUOLES OF RED BEET ROOT CELLS

Tsitologiya ◽  
2018 ◽  
Vol 60 (6) ◽  
pp. 469-475
Author(s):  
O. D. Nimaeva ◽  
◽  
E. V. Pradedova ◽  
A. B. Karpova ◽  
R. K. Salyaev ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Root Cells ◽  
Beet Root ◽  
Red Beet ◽  
Alcohol Dehydrogenase Activity
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