scholarly journals Interrelationships between lysine and α∈-diaminopimelic acid and their derivatives and analogues in mutants of Escherichia coli

1957 ◽  
Vol 66 (2) ◽  
pp. 270-282 ◽  
Author(s):  
Pauline Meadow ◽  
D. S. Hoare ◽  
Elizabeth Work
Keyword(s):  
Escherichia Coli ◽  
Diaminopimelic Acid

Effect of analogues of diaminopimelic acid on the meso -diaminopimelate-adding enzyme from Escherichia coli

FEBS Letters ◽  
1996 ◽  
Vol 391 (1-2) ◽  
pp. 171-174 ◽  
Author(s):  
Geneviève Auger ◽  
Jean van Heijenoort ◽  
John C. Vederas ◽  
Didier Blanot
Keyword(s):  
Escherichia Coli ◽  
Diaminopimelic Acid

scholarly journals Inhibition of Escherichia coli growth and diaminopimelic acid epimerase by 3-chlorodiaminopimelic acid.

1988 ◽  
Vol 32 (8) ◽  
pp. 1119-1123 ◽  
Author(s):  
R J Baumann ◽  
E H Bohme ◽  
J S Wiseman ◽  
M Vaal ◽  
J S Nichols
Keyword(s):  
Escherichia Coli ◽  
Diaminopimelic Acid ◽  
Coli Growth

THE CONVERSION OF 2,6-DIAMINOPIMELIC ACID-1,7-C14TO LYSINE-1-C14BY CERTAIN BACTERIA

1961 ◽  
Vol 39 (10) ◽  
pp. 1551-1558 ◽  
Author(s):  
A. J. Finlayson ◽  
F. J. Simpson
Keyword(s):  
Escherichia Coli ◽  
Aspergillus Flavus ◽  
Culture Medium ◽  
Leuconostoc Mesenteroides ◽  
Streptomyces Griseus ◽  
Diaminopimelic Acid ◽  
Proteus Vulgaris ◽  
E Coli ◽  
Carbon 14 ◽  
Carboxyl Carbon

When 2,6-diaminopimelicacid-1,7-C14was added to growing cultures of Bacillus megaterium, Staphlococcus aureus, and Escherichia coli, 8–9% of added carbon-14 appeared in the cellular lysine. Similar experiments with Proteus vulgaris, Streptomyces griseus, Aspergillus flavus, and Lactobacillus arabinosus resulted in less than 0.3% of the added carbon-14 being incorporated into the cellular lysine. Leuconostoc mesenteroides converted 0.6% of the added DAP-1,7-C14to lysine-1-C14.Over 90% of the carbon-14 in cell lysine from B. megaterium and L. mesenteroides was found in the carboxyl carbon. This was interpreted as indicating a direct decarboxylation of DAP-1,7-C14to lysine-1-C14. About 70% of the carbon-14 in the lysine from cells of S. aureus and E. coli was found in the carboxyl carbon, thus suggesting that some lysine comes from sources other than 2,6-diaminopimelic acid.Those organisms that actively decarboxylated DAP-1,7-C14to form lysine-C14also synthesized DAP and excreted it into the culture medium during growth.

Inhibition of uridine 5′-diphosphate-N-acetylmuramyl-L-alanine synthetase by β-chloro-L-alanine in Escherichia coli

1982 ◽  
Vol 28 (6) ◽  
pp. 654-659 ◽  
Author(s):  
Edward E. Ishiguro
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Diaminopimelic Acid ◽  
Alanine Racemase ◽  
Uridine Diphosphate ◽  
Amino Acid Deprivation ◽  
Intact Cells ◽  
Low Concentration ◽  
Low Concentrations ◽  
Precursor Synthesis

The synthesis of the nucleotide precursors for peptidoglycan is regulated by the relA gene in Escherichia coli. Thus, nucleotide precursors labeled with [3H]diaminopimelic acid accumulated in a relA strain but not in an isogenic relA+ strain during amino acid deprivation. Furthermore, nucleotide precursor synthesis was relaxed in the amino acid deprived relA+ strain by treatment with chloramphenicol. Uridine diphosphate-N-acetylmuramyl-pentapeptide (UDP-MurNAc-pentapeptide) was the major component accumulated during the relaxed synthesis of nucleotide precursors in both relA+ and relA strains. The effect of β-chloro-L-alanine (CLA) on the relaxed synthesis of nucleotide precursors for peptidoglycan was determined. At a low concentration (0.0625 mM) CLA inhibited the synthesis of UDP-MurNAc-pentapeptide and caused the accumulation of UDP-MurNAc-tripeptide. Thus, low concentrations of CLA probably inhibited alanine racemase, as reported previously. Higher concentrations of CLA also inhibited an earlier step in nucleotide precursor synthesis. This was shown to be due to the inhibition of UDP-MurNAc-L-alanine synthetase by CLA. CLA inhibited the activity of this enzyme in cell-free extracts as well as in intact cells.

Growth and cell division of Escherichia coli 173-25 in the presence of some analogues of diaminopimelic acid

2007 ◽  
Vol 14 (4) ◽  
pp. 283-296
Author(s):  
J. Chaloupka ◽  
M. Strnadová ◽  
J. Čáslavská ◽  
K. Vereš
Keyword(s):  
Escherichia Coli ◽  
Cell Division ◽  
Diaminopimelic Acid

scholarly journals Antifungal and antibacterial activities of Streptomyces polymachus sp. nov. isolated from soil

2015 ◽  
Vol 65 (Pt_8) ◽  
pp. 2385-2390 ◽  
Author(s):  
Tuan Manh Nguyen ◽  
Jaisoo Kim
Keyword(s):  
Escherichia Coli ◽  
Type Strain ◽  
Novel Species ◽  
Aerial Mycelium ◽  
Antibacterial Activities ◽  
Potato Dextrose Agar ◽  
Diaminopimelic Acid ◽  
Melanin Production ◽  
Substrate Mycelium ◽  
Antimicrobial And Antifungal Activity

Strain T258T was isolated from forest soil at Bongnae Falls, South Korea. The strain exhibited antimicrobial and antifungal activity against the following strains: Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Paenibacillus larvae, Escherichia coli, Candida albicans and Aspergillus niger. Growth occurred on all ISP media tested (2, 3, 4, 5, 6 and 7), Czapek–Dox agar, potato dextrose agar, trypticase soy agar, Bennett's modified agar and nutrient agar at 28 °C. Aerial spores were produced solely on ISP Medium 4; the colour of the aerial mycelium was white and the substrate mycelium was ivory. Melanin production was negative on peptone–yeast extract iron agar (ISP Medium 6). The cell-wall peptidoglycan contained ll-diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates contained glucose, ribose and galactose. The predominant menaquinones were MK-9(H6) and MK-9(H8) while the minor menaquinone was MK-10(H2). The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids (>10 %) were C16 : 0 (29.8 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (15.1 %), anteiso-C15 : 0 (13.5 %) and iso-C15 : 0 (10.3 %). DNA–DNA similarity with other strains ranged between 37.84 ± 1.15 % and 50.25 ± 1.91 %. On the basis of these data, we suggest that strain T258T represents a novel species that belong to the genus Streptomyces, for which we propose a name Streptomyces polymachus sp. nov. The type strain is T258T ( = KACC 18247T = KEMB 9005-212T = NBRC 110905T).

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