Effect of diaminopimelic acid-limited growth on the peptidoglycan composition of Escherichia coli W7

1985 ◽  
Vol 51 (5-6) ◽  
pp. 556-556 ◽  
Author(s):  
F. Driehuis ◽  
J. T. M. Wouters
Keyword(s):  
Escherichia Coli ◽  
Diaminopimelic Acid ◽  
Limited Growth

Effect of analogues of diaminopimelic acid on the meso -diaminopimelate-adding enzyme from Escherichia coli

FEBS Letters ◽  
1996 ◽  
Vol 391 (1-2) ◽  
pp. 171-174 ◽  
Author(s):  
Geneviève Auger ◽  
Jean van Heijenoort ◽  
John C. Vederas ◽  
Didier Blanot
Keyword(s):  
Escherichia Coli ◽  
Diaminopimelic Acid

scholarly journals The lytB Gene of Escherichia coli Is Essential and Specifies a Product Needed for Isoprenoid Biosynthesis

2001 ◽  
Vol 183 (24) ◽  
pp. 7403-7407 ◽  
Author(s):  
Sean McAteer ◽  
Andrew Coulson ◽  
Neil McLennan ◽  
Millicent Masters
Keyword(s):  
Escherichia Coli ◽  
Protein Structure ◽  
Cell Lysis ◽  
Isoprenoid Biosynthesis ◽  
Globular Protein ◽  
Limited Growth ◽  
E Coli ◽  
Nonmevalonate Pathway ◽  
Tim Barrel

ABSTRACT LytB and GcpE, because they are codistributed with other pathway enzymes, have been predicted to catalyze unknown steps in the nonmevalonate pathway for isoprenoid biosynthesis. We constructed a conditional Escherichia coli lytB mutant and found that LytB is essential for survival and that depletion of LytB results in cell lysis, which is consistent with a role for this protein in isoprenoid biosynthesis. Alcohols which can be converted to pathway intermediates beyond the hypothesized LytB step(s) support limited growth of E. coli lytB mutants. An informatic analysis of protein structure suggested that GcpE is a globular protein of the TIM barrel class and that LytB is also a globular protein. Possible biochemical roles for LytB and GcpE are suggested.

Growth of Selected Cross-Contaminating Bacterial Pathogens on Beef and Fish at 15 and 35°C+

1994 ◽  
Vol 57 (4) ◽  
pp. 337-340 ◽  
Author(s):  
AJIBOLA O. FAPOHUNDA ◽  
KENNETH W. MCMILLIN ◽  
DOUGLAS L. MARSHALL ◽  
W. M. WAITES
Keyword(s):  
Escherichia Coli ◽  
Clostridium Perfringens ◽  
Aeromonas Hydrophila ◽  
Growth Response ◽  
Cross Contamination ◽  
Limited Growth ◽  
E Coli ◽  
Fish Samples ◽  
Conditioning Temperature ◽  
The Tropics

Isolates of Escherichia coli and Clostridium perfringens from beef and Aeromonas hydrophila from fish were examined for their ability to survive and grow as cross-contaminates on nonnative tissues at simulated ambient (35°C) and aging/conditioning (15°C) temperatures of handling and retailing found in the tropics. Growth of all isolates over a 10-h period was greater (P < 0.05) on their native tissues at both temperatures. The aging/conditioning temperature effectively limited growth of E. coli and A. hydrophila to less than l-logl0 CFU/g and prevented growth of C. perfringens on beef and fish samples. All three isolates demonstrated characteristic mesophilic growth response on both tissues at 35°C during the 10-h retail period. The study suggests that two muscle food products could be jointly handled to efficiently use available storage/haulage capacity in tropical countries. Potential savings in space, labor and energy would be made if cross-contamination between the two products is minimized by available packaging and sanitizing technologies.

scholarly journals Inhibition of Escherichia coli growth and diaminopimelic acid epimerase by 3-chlorodiaminopimelic acid.

1988 ◽  
Vol 32 (8) ◽  
pp. 1119-1123 ◽  
Author(s):  
R J Baumann ◽  
E H Bohme ◽  
J S Wiseman ◽  
M Vaal ◽  
J S Nichols
Keyword(s):  
Escherichia Coli ◽  
Diaminopimelic Acid ◽  
Coli Growth

THE CONVERSION OF 2,6-DIAMINOPIMELIC ACID-1,7-C14TO LYSINE-1-C14BY CERTAIN BACTERIA

1961 ◽  
Vol 39 (10) ◽  
pp. 1551-1558 ◽  
Author(s):  
A. J. Finlayson ◽  
F. J. Simpson
Keyword(s):  
Escherichia Coli ◽  
Aspergillus Flavus ◽  
Culture Medium ◽  
Leuconostoc Mesenteroides ◽  
Streptomyces Griseus ◽  
Diaminopimelic Acid ◽  
Proteus Vulgaris ◽  
E Coli ◽  
Carbon 14 ◽  
Carboxyl Carbon

When 2,6-diaminopimelicacid-1,7-C14was added to growing cultures of Bacillus megaterium, Staphlococcus aureus, and Escherichia coli, 8–9% of added carbon-14 appeared in the cellular lysine. Similar experiments with Proteus vulgaris, Streptomyces griseus, Aspergillus flavus, and Lactobacillus arabinosus resulted in less than 0.3% of the added carbon-14 being incorporated into the cellular lysine. Leuconostoc mesenteroides converted 0.6% of the added DAP-1,7-C14to lysine-1-C14.Over 90% of the carbon-14 in cell lysine from B. megaterium and L. mesenteroides was found in the carboxyl carbon. This was interpreted as indicating a direct decarboxylation of DAP-1,7-C14to lysine-1-C14. About 70% of the carbon-14 in the lysine from cells of S. aureus and E. coli was found in the carboxyl carbon, thus suggesting that some lysine comes from sources other than 2,6-diaminopimelic acid.Those organisms that actively decarboxylated DAP-1,7-C14to form lysine-C14also synthesized DAP and excreted it into the culture medium during growth.

scholarly journals Effects of sulphate-limited growth in continuous culture on the electron-transport chain and energy conservation in Escherichia coli K12

1975 ◽  
Vol 152 (3) ◽  
pp. 537-546 ◽  
Author(s):  
R K Poole ◽  
B A Haddock
Keyword(s):  
Escherichia Coli ◽  
Electron Transport ◽  
Electron Transport Chain ◽  
Functional Organization ◽  
Extracellular Polysaccharide ◽  
Limited Growth ◽  
Escherichia Coli K12 ◽  
Transport Chain ◽  
Respiratory Chains ◽  
Energy Dependent

Growth of Escherichia coli K12 in a chemostat was limited by sulphate concentrations lower than 300 muM. The synthesis of extracellular polysaccharide and a change in morphology accompanied sulphate-limited growth. Growth yields with respect to the amount of glycerol or oxygen consumed were sixfold and twofold lower respectively under these conditions than when growth was limited by glycerol. Sulphate-limited cells lacked the proton-translocating oxidoreduction segment of the electron-transport chain between NADH and the cytochromes, and particles prepared from these cells lacked the energy-dependent reduction of NAD+ by succinate, DL-α-glycerophosphate or D-lactate, suggesting the loss of site-I phosphorylation. Glycerol-limited cells contained cytochrome b556, b562 and o, ubiquinone and low concentrations of menaquinone. Sulphate limitation resulted in the additional synthesis of cytochromes d, a1, b558 and c550; the amount of ubiquinone was decreased and menaquinone was barely detectable. Non-haem iron and acid-labile sulphide concentrations were twofold lower in electron-transport particles prepared from sulphate-limited cells. Recovery of site-I phosphorylation could not be demonstrated after incubating sulphate-limited cells with or without glycerol, in either the absence or presence of added sulphate. The loss of site-I phosphorylation in sulphate-limited cells is discussed with reference to the accompanying alterations in cytochrome composition of such cells. Schemes are proposed for the functional organization of the respiratory chains of E. coli grown under conditions of glycerol or sulphate limitation.

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