scholarly journals A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid

1956 ◽  
Vol 62 (2) ◽  
pp. 315-323 ◽  
Author(s):  
K. Burton
Keyword(s):  
Deoxyribonucleic Acid ◽  
Colorimetric Estimation ◽  
Diphenylamine Reaction

scholarly journals A temperate bacteriophage of Clostridium perfringens

1968 ◽  
Vol 14 (10) ◽  
pp. 1085-1093 ◽  
Author(s):  
D. E. Mahony ◽  
G. G. Kalz
Keyword(s):  
Electron Microscopy ◽  
Clostridium Perfringens ◽  
Deoxyribonucleic Acid ◽  
Solid Medium ◽  
Temperate Bacteriophage ◽  
Acridine Orange Staining ◽  
Lysogenic Strain ◽  
Step Growth ◽  
Growth Experiments ◽  
Diphenylamine Reaction

A temperate bacteriophage was isolated from a lysogenic strain of Clostridium perfringens. This lysogenic strain was inducible by ultraviolet light. Plaques produced on solid medium were small with turbid centers. Electron microscopy revealed that the phage has a polyhedral head, 80 × 70 mμ, and a striated tail, 200 mμ long.One-step growth experiments revealed latent and release periods of 45 minutes each. Phage yields were variable but, in general, quite low. The adsorption rate and temperature and pH stability of the phage were determined.The diphenylamine reaction and acridine orange staining suggested that the phage nucleic acid is deoxyribonucleic acid.

MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Oil Spill ◽  
Deoxyribonucleic Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Degradation ◽  
Sequence Determination ◽  
Blast Search ◽  
Degrading Bacteria ◽  
Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

Sign in / Sign up

Export Citation Format

Share Document