scholarly journals The effect of adrenal cortical preparations added in vitro upon the carbohydrate metabolism of liver slices. 2. The effect of some pure steroids upon carbohydrate synthesis, oxygen uptake and non-protein nitrogen

1950 ◽  
Vol 46 (1) ◽  
pp. 120-124 ◽  
Author(s):  
C. Y. Chiu
Keyword(s):  
Oxygen Uptake ◽  
Carbohydrate Metabolism ◽  
Protein Nitrogen ◽  
Carbohydrate Synthesis ◽  
Liver Slices

scholarly journals Oxygen uptake and carbohydrate metabolism by in vitro derived bovine embryos

Reproduction ◽  
1996 ◽  
Vol 106 (2) ◽  
pp. 299-306 ◽  
Author(s):  
J. G. Thompson ◽  
R. J. Partridge ◽  
F. D. Houghton ◽  
C. I. Cox ◽  
H. J. Leese
Keyword(s):  
Oxygen Uptake ◽  
Carbohydrate Metabolism ◽  
Bovine Embryos

Effect of in Vitro Administration of Thyroxine and Triiodothyronine on Surviving Liver Slices

1956 ◽  
Vol 185 (2) ◽  
pp. 243-244 ◽  
Author(s):  
A. Kurt Weiss
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Guinea Pig ◽  
Liver Slices ◽  
Suspension Medium ◽  
Phosphate Medium

Liver slices from frog, turtle, guinea pig, greyhound and man were incubated in Krebs-Ringer-Phosphate medium and their oxygen uptake determined manometrically. The addition of l-thyroxine or l-triiodothyronine to the suspension medium did not produce an increased oxygen consumption.

CORTICOSTEROID-UMSATZ UND CORTICOSTEROID-PRODUKTION BEI RATTEN UNTER DER BEHANDLUNG MIT ANABOLEN STEROIDEN

1965 ◽  
Vol 48 (2) ◽  
pp. 263-271 ◽  
Author(s):  
Herbert Schriefers ◽  
Gerlinde Scharlau ◽  
Franzis Pohl
Keyword(s):  
Production Rate ◽  
Adult Female ◽  
Anabolic Steroids ◽  
Reduction Rate ◽  
Female Rats ◽  
Adrenal Weight ◽  
Hydrogenase Activity ◽  
Anabolic Effect ◽  
Liver Slices

ABSTRACT After the administration of anabolic steroids to adult female rats in daily doses of 1 mg per animal for 14 days, the following parameters were investigated: the rate of the Δ4-5α-hydrogenase-catalyzed cortisone reduction in liver slices and microsomal fractions, the adrenal weight and the in vitro corticosterone production rate. Among the steroids tested, only 17α-methyl-testosterone and 17α-ethyl-19-nor-testosterone were effective in lowering significantly cortisone reduction rate by liver slices with concomitant decreases in microsomal Δ4-5α-hydrogenase-activity. Testosterone, 19-nor-testosterone, 17α-ethinyl-19-nor-testosterone, 17α-methyl-17β-hydroxy-androsta-1,4-dien-3-one and 1-methyl-17β-hydroxy-androst-1-en-3-one were ineffective or only slightly effective. Adrenal weight and absolute corticosterone production rate (μg/60 min per animal) were decreased after treatment with 17α-methyl-testosterone, 17α-ethyl-19-nor-testosterone and 1-methyl-17β-hydroxy-androst-1-en-3-one. Corticosterone production was decreased with 17α-ethinyl-19-nor-testosterone in spite of an unchanged adrenal weight. The relative corticosterone production rate (μg/60 min · 100 mg adrenal) was in any cases unaffected. According to these results there exists – with the exception of 17α-ethinyl-19-nor-testosterone – a strict parallelism between corticosteroid turnover and corticosterone production rate: unchanged turnover is correlated with unchanged corticosterone production rate, while a decreased turnover is correlated with decreased adrenal activity. The protein-anabolic effect of certain anabolic steroids may be partly due to an anti-catabolic action of these compounds resulting from a decreased corticosteroid inactivation and production rate. Possible mechanisms by which anabolic steroids may affect corticosteroid-balance are discussed.

Insulin-like action of proinsulin on rat liver carbohydrate metabolism in vitro

Diabetes ◽  
1985 ◽  
Vol 34 (5) ◽  
pp. 415-419 ◽  
Author(s):  
I. Probst ◽  
H. Hartmann ◽  
K. Jungermann ◽  
W. Creutzfeldt
Keyword(s):  
Carbohydrate Metabolism ◽  
Rat Liver

Precision-cut Guinea-pig Liver Slices as a Tool for Studying the Toxicity of Volatile Anaesthetics

1990 ◽  
Vol 18 (1_part_1) ◽  
pp. 191-199
Author(s):  
Hanan N. Ghantous ◽  
Jeanne Fernando ◽  
Scott E. Morgan ◽  
A. Jay Gandolfi ◽  
Klaus Brandel
Keyword(s):  
Guinea Pig ◽  
Rank Order ◽  
Normal Liver ◽  
Liver Slice ◽  
Volatile Anaesthetics ◽  
Pig Liver ◽  
Liver Slices ◽  
Guinea Pig Liver ◽  
Krebs Henseleit Buffer

Cultured precision-cut liver slices retain normal liver architecture and physiological biochemical functions. Hartley male guinea-pig liver slices have proven to be a good model for studying the biotransformation and toxicity of halothane. This system was used to evaluate the biotransformation and toxicity of different volatile anaesthetics (halothane, enflurane, isoflurane and sevoflurane), and compare their effects to those of new anaesthetics (desflurane). Liver slices (250–300μm thick) were incubated in sealed roller vials, containing Krebs Henseleit buffer at 37°C under 95% O2:5% CO2 atmosphere. Volatile anaesthetics were delivered by volatilisation after pre-incubation for 1 hour to produce a constant concentration in the medium. Production of the metabolites, trifluroacetic acid and fluoride ion, was measured. Intracellular potassium ion content, protein synthesis and secretion were determined as indicators of viability of the slices. The rank order of biotransformation of anaesthetics by the liver slices was halothane >sevoflurane>isoflurane and enflurane>desflurane. The rank order of hepatotoxicity of these anaesthetics was halothane>isoflurane and enflurane>sevoflurane and desflurane. Halothane is the anaesthetic which is metabolised furthest and has the most toxic effect, while desflurane is the least metabolised anaesthetic and has the least toxicity. This in vitro cultured precision-cut liver slice system appears to be suitable for studying the biotransformation of volatile anaesthetics and correlating its role in the resulting toxicity.

scholarly journals THE EFFECT OF LEAD ACETATE ON OXYGEN UPTAKE OF RAT LIVER SLICES

1941 ◽  
Vol 140 (1) ◽  
pp. 285-291
Author(s):  
Harry D. Baernstein ◽  
J.A. Grand
Keyword(s):  
Oxygen Uptake ◽  
Rat Liver ◽  
Lead Acetate ◽  
Liver Slices
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