scholarly journals INFLUENCE OF MESOXALATE AND VARIOUS HORMONES ON CARBOHYDRATE METABOLISM IN RAT LIVER SLICES (IN VITRO)

1956 ◽  
Vol 3 (1) ◽  
pp. 23-31 ◽  
Author(s):  
ROKURO NAKAI
Keyword(s):  
Carbohydrate Metabolism ◽  
Rat Liver ◽  
Liver Slices

Insulin-like action of proinsulin on rat liver carbohydrate metabolism in vitro

Diabetes ◽  
1985 ◽  
Vol 34 (5) ◽  
pp. 415-419 ◽  
Author(s):  
I. Probst ◽  
H. Hartmann ◽  
K. Jungermann ◽  
W. Creutzfeldt
Keyword(s):  
Carbohydrate Metabolism ◽  
Rat Liver

scholarly journals STUDIES ON CARBOHYDRATE METABOLISM IN RAT LIVER SLICES

1958 ◽  
Vol 230 (2) ◽  
pp. 761-771 ◽  
Author(s):  
Robert G. Spiro ◽  
James Ashmore ◽  
A. Baird Hastings
Keyword(s):  
Carbohydrate Metabolism ◽  
Rat Liver ◽  
Liver Slices

Studies on the induction of rat hepatic CYP1A, CYP2B, CYP3A and CYP4A subfamily form mRNAs in vivo and in vitro using precision-cut rat liver slices

Xenobiotica ◽  
2003 ◽  
Vol 33 (5) ◽  
pp. 511-527 ◽  
Author(s):  
C. Meredith ◽  
M. P. Scott ◽  
A. B. Renwick ◽  
R. J. Price ◽  
B. G. Lake
Keyword(s):  
Rat Liver ◽  
Liver Slices

Insulin stimulus of leucine incorporation into frog liver protein

1962 ◽  
Vol 203 (4) ◽  
pp. 687-689 ◽  
Author(s):  
J. C. Penhos ◽  
M. E. Krahl
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Leucine Incorporation ◽  
Liver Protein ◽  
Amino Acid Incorporation ◽  
Insulin Effect ◽  
Acid Incorporation ◽  
Liver Slices ◽  
Stimulation Of

Slices prepared from livers of bull frogs ( Rana catesbiana), pancreatectomized and/or hypophysectomized 7 days before, were incubated 2 hr in frog Ringer-bicarbonate solution at 25 C. Incorporation of leucine-1-C14 into protein was subnormal in the pancreatectomized series. The addition of insulin in vitro, with glucose also present in the medium, produced a significant ( P < 0.01) stimulation of amino acid incorporation in the following series: livers from normal fed animals; livers from animals pancreatectomized 7 days before; and livers from animals pancreatectomized and hypophysectomized 7 days before. Neither insulin nor glucose alone gave a significant effect. These results therefore confirm and extend those obtained with rat liver slices showing that insulin can stimulate amino acid incorporation into protein when added directly to liver. The effect is relatively greatest with livers from animals pancreatectomized 7 days before; the insulin effect does not depend on the presence of the pituitary, as it is obtainable with livers from animals hypophysectomized and pancreatectomized 7 days previously.

Gene Expression Profiles in Rat Liver Slices Exposed to Hepatocarcinogenic Enzyme Inducers, Peroxisome Proliferators, and 17α-Ethinylestradiol

2006 ◽  
Vol 25 (5) ◽  
pp. 379-395 ◽  
Author(s):  
Gisela Werle-Schneider ◽  
Andreas Wölfelschneider ◽  
Marie Charlotte von Brevern ◽  
Julia Scheel ◽  
Thorsten Storck ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Mode Of Action ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Peroxisome Proliferators ◽  
Liver Slices ◽  
Enzyme Inducers

Transcription profiling is used as an in vivo method for predicting the mode-of-action class of nongenotoxic carcinogens. To set up a reliable in vitro short-term test system DNA microarray technology was combined with rat liver slices. Seven compounds known to act as tumor promoters were selected, which included the enzyme inducers phenobarbital, α-hexachlorocyclohexane, and cyproterone acetate; the peroxisome proliferators WY-14,643, dehydroepiandrosterone, and ciprofibrate; and the hormone 17 α-ethinylestradiol. Rat liver slices were exposed to various concentrations of the compounds for 24 h. Toxicology-focused TOXaminer™ DNA microarrays containing approximately 1500 genes were used for generating gene expression profiles for each of the test compound. Hierarchical cluster analysis revealed that (i) gene expression profiles generated in rat liver slices in vitro were specific allowing classification of compounds with similar mode of action and (ii) expression profiles of rat liver slices exposed in vitro correlate with those induced after in vivo treatment (reported previously). Enzyme inducers and peroxisome proliferators formed two separate clusters, confirming that they act through different mechanisms. Expression profiles of the hormone 17 α-ethinylestradiol were not similar to any of the other compounds. In conclusion, gene expression profiles induced by compounds that act via similar mechanisms showed common effects on transcription upon treatment in vivo and in rat liver slices in vitro.

scholarly journals Release of Folate by Rat Liver and Spleen Slices

Blood ◽  
1969 ◽  
Vol 33 (3) ◽  
pp. 400-407 ◽  
Author(s):  
ERIK M. MAGNUS ◽  
HUGH DEMPSEY ◽  
C. E. BUTTERWORTH
Keyword(s):  
Rat Liver ◽  
Test Organism ◽  
Rat Plasma ◽  
Bathing Solution ◽  
Liver Slices ◽  
Insoluble Form ◽  
High Concentrations ◽  
Chicken Pancreas ◽  
Liver Folate

Abstract 1. The effects of rat plasma and chicken pancreas have been appraised concerning their role in releasing bound folate from rat liver and spleen slices in vitro. Net changes in folate concentration of the bathing solution have been determined by bacteriologic assay employing L. casei as the test organism. 2. Both rat plasma and chicken pancreas extract enhance the release of folate from liver slices in comparison with saline controls. Rat plasma was as effective in liberating bound folate from liver as a 1:25 dilution of conventionally-prepared chicken pancreas extract. The evidence suggests that a considerable portion of liver folate exists in an insoluble form which is bound to cellular structures. 3. Mature red cells appeared to be impermeable to high concentrations of natural folate released from liver. 4. Spleen slices released folate to the supernatant fluid when incubated with chicken pancreas extract, although at levels approximately one-tenth as great as liver slices. When spleen slices were incubated for short periods with plasma, evidence was obtained favoring a flux of folate into surviving cells.

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