scholarly journals The hydrolysis of the combined forms of oestrone and oestriol present in human pregnancy urine

1935 ◽  
Vol 29 (7) ◽  
pp. 1577-1585 ◽  
Author(s):  
Saul Louis Cohen ◽  
Guy Frederic Marrian
Keyword(s):  
Human Pregnancy ◽  
Pregnancy Urine ◽  
Hydrolysis Of

HYDROLYSIS OF CONJUGATED ESTROGEN FRACTIONS IN HUMAN PREGNANCY URINE

1961 ◽  
Vol 39 (10) ◽  
pp. 1501-1514 ◽  
Author(s):  
Sidsel Bugge ◽  
Mona Nilsen ◽  
Ann Metcalfe-Gibson ◽  
R. Hobkirk
Keyword(s):  
Helix Pomatia ◽  
Enzyme Hydrolysis ◽  
Digestive Juice ◽  
Human Pregnancy ◽  
Mammalian Liver ◽  
Pregnancy Urine ◽  
Snail Enzyme ◽  
17Β Estradiol ◽  
Snail Helix Pomatia ◽  
Hydrolysis Of

The release of six estrogen fractions from conjugation in human pregnancy urines has been studied using various hydrolytic methods. The estrogens concerned were estrone, estradiol-17β (estradiol), 2-methoxyestrone, 16-epiestriol, and a ring D ketolic fraction (mainly 16α-hydroxyestrone). Considerable amounts of urinary estrone and ring D ketolic estrogens may be conjugated in a non-glucuronide form. In these cases an enzyme preparation containing β-glucuronidase and sulphatase, prepared from the digestive juice of the snail Helix pomatia, proved to be superior to β-glucuronidase enzymes of bacterial or mammalian liver origin. Conventional hot acid hydrolysis yielded levels of estrone, estradiol, estriol, and 16-epiestriol which agreed fairly well with those obtained following snail enzyme hydrolysis. In some urines, hot acid treatment was not suitable for hydrolysis of conjugated 2-methoxyestrone. Optimum hydrolytic conditions for both normal and diabetic pregnancy urines were realized by incubating for 24 hours with 500 units of the snail β-glucuronidase and 250 units of sulphatase/ml of urine at pH 5.2 and 37–38 °C.

COMPARATIVE STUDIES ON HYDROLYSIS OF CONJUGATED ESTROGENS IN HUMAN PREGNANCY URINE

Endocrinology ◽  
1955 ◽  
Vol 57 (1) ◽  
pp. 87-95 ◽  
Author(s):  
RICHARD F. STRAW ◽  
PHILIP A. KATZMAN ◽  
EDWARD A. DOISY
Keyword(s):  
Comparative Studies ◽  
Conjugated Estrogens ◽  
Human Pregnancy ◽  
Pregnancy Urine ◽  
Hydrolysis Of

STEROLS. XII. SYNTHETIC PREPARATION OF EPI-ALLO-PREGNANOLONE, THE ANDROGENIC PRINCIPLE OF HUMAN PREGNANCY URINE

1937 ◽  
Vol 59 (4) ◽  
pp. 768-768
Author(s):  
Russell E. Marker ◽  
Oliver Kamm ◽  
David M. Jones ◽  
Eugene L. Wittle ◽  
Thomas S. Oakwood ◽  
...  
Keyword(s):  
Human Pregnancy ◽  
Pregnancy Urine

Conjugation of phenolic steroid ring Dα-ketols in human pregnancy urine

1971 ◽  
Vol 31 (2) ◽  
pp. 387-393 ◽  
Author(s):  
S.Y. Tan ◽  
Y. Anuman-Rajadhon ◽  
R. Hobkirk
Keyword(s):  
Human Pregnancy ◽  
Pregnancy Urine
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