scholarly journals The determination of the membrane potentials of protein solutions and the valence of protein ions

1934 ◽  
Vol 28 (1) ◽  
pp. 199-221 ◽  
Author(s):  
Gilbert Smithson Adair ◽  
Muriel Elaine Adair
Keyword(s):  
Membrane Potentials ◽  
Protein Solutions ◽  
Protein Ions

scholarly journals Determination of transmembrane pH gradients and membrane potentials in liposomes

1992 ◽  
Vol 63 (5) ◽  
pp. 1336-1345 ◽  
Author(s):  
P.R. Harrigan ◽  
M.J. Hope ◽  
T.E. Redelmeier ◽  
P.R. Cullis
Keyword(s):  
Membrane Potentials ◽  
Ph Gradients

Intermolecular interactions with/within cell membranes and the trinity of membrane potentials: kinetics and imaging

2003 ◽  
Vol 31 (5) ◽  
pp. 990-996 ◽  
Author(s):  
P. O'Shea
Keyword(s):  
Intermolecular Interactions ◽  
Cell Biology ◽  
Membrane Surface ◽  
Membrane Potentials ◽  
Membrane Microdomains ◽  
Membrane Dipole Potential ◽  
Additional Level ◽  
The Trinity ◽  
Detection Technologies

The interactions of (macro-)molecules with biological membranes underlies much of cell biology. This paper outlines many of the factors that must be taken into account in order to understand fully the nature of these interactions. These include some roles of the membrane potentials including features of the surface and dipole potentials. Several fluorescence detection technologies directed towards these are outlined that offer high-resolution experimental determination of the intermolecular interactions by measuring small changes of these potentials resulting from specific interactions of many kinds of molecular species. The possibilities for making single-cell spatial imaging measurements of such interactions is also described. Examples are used to indicate the feasibility of identifying and tracking localized interactions on the membrane surface in real-time. Some of this work points to the possibility that the membrane dipole potential spatially varies about the cell surface, particularly within membrane microdomains such as ‘rafts’. Such variation is suggested to underlie the altered behaviour of signalling systems within rafts and offer the means of an additional level of biological control.

Experimental Determination of Microsecond Reorientation Correlation Times in Protein Solutions

2013 ◽  
Vol 117 (13) ◽  
pp. 3548-3553 ◽  
Author(s):  
Enrico Ravera ◽  
Giacomo Parigi ◽  
Andi Mainz ◽  
Tomasz L. Religa ◽  
Bernd Reif ◽  
...  
Keyword(s):  
Experimental Determination ◽  
Protein Solutions ◽  
Correlation Times

scholarly journals STUDIES ON THE ANOMALOUS VISCOSITY AND FLOW-BIREFRINGENCE OF PROTEIN SOLUTIONS

1944 ◽  
Vol 27 (3) ◽  
pp. 201-232 ◽  
Author(s):  
A. S. C. Lawrence ◽  
Joseph Needham ◽  
Shih-Chang Shen
Keyword(s):  
Simultaneous Determination ◽  
Surface Film ◽  
Flow Birefringence ◽  
Bulk Phase ◽  
Protein Solutions ◽  
Dilute Solutions ◽  
General Behaviour ◽  
Anomalous Viscosity

1. A coaxial viscosimeter which permits the simultaneous determination of relative and anomalous viscosity and of flow-birefringence is described. Flow-anomaly and flow-birefringence are regarded as characteristic of elongated micelles and molecules. 2. Such methods have been applied to dilute solutions of proteins. The conditions under which the coaxial (Couette) viscosimeter measures the viscosity of the bulk phase and the surface film phase respectively have been investigated and are described. 3. The general behaviour of protein solutions subjected to shear is summarised.

A universal method for the determination of polysorbate 80 in monoclonal antibodies and novel protein therapeutic formulations

2018 ◽  
Vol 10 (11) ◽  
pp. 1296-1304 ◽  
Author(s):  
Veerendra Koppolu ◽  
Bhargavi Vemulapalli ◽  
Jason Thomas ◽  
Sheau-Chiann Wang ◽  
Jon Borman
Keyword(s):  
Monoclonal Antibodies ◽  
Sample Preparation ◽  
Protein Solutions ◽  
Polysorbate 80 ◽  
Universal Method ◽  
Minimal Sample ◽  
Protein Therapeutic ◽  
Novel Protein

A universal and simple assay requiring minimal sample preparation has been developed to quantitate PS80 and other excipients in protein solutions.

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