A universal method for the determination of polysorbate 80 in monoclonal antibodies and novel protein therapeutic formulations

2018 ◽  
Vol 10 (11) ◽  
pp. 1296-1304 ◽  
Author(s):  
Veerendra Koppolu ◽  
Bhargavi Vemulapalli ◽  
Jason Thomas ◽  
Sheau-Chiann Wang ◽  
Jon Borman
Keyword(s):  
Monoclonal Antibodies ◽  
Sample Preparation ◽  
Protein Solutions ◽  
Polysorbate 80 ◽  
Universal Method ◽  
Minimal Sample ◽  
Protein Therapeutic ◽  
Novel Protein

A universal and simple assay requiring minimal sample preparation has been developed to quantitate PS80 and other excipients in protein solutions.

scholarly journals Determination of 18 Intact Glucosinolates in Brassicaceae Vegetables by UHPLC-MS/MS: Comparing Tissue Disruption Methods for Sample Preparation

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 231
Author(s):  
Xiaolu Yu ◽  
Hongju He ◽  
Xuezhi Zhao ◽  
Guangmin Liu ◽  
Liping Hu ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Dry Weight ◽  
Microwave Treatment ◽  
Universal Method ◽  
Anticancer Activities ◽  
Fresh Sample ◽  
Freeze Dried ◽  
Relative Standard ◽  
Quantification Accuracy

Glucosinolates (GSLs) are important precursor compounds with anticancer activities in Brassicaceae vegetables and are readily hydrolyzed by myrosinase. Given the diversity of these species, establishing an accurate and universal method to quantify intact GSLs in different plant tissues is necessary. Here, we compared and optimized three tissue disruption methods for sample preparation. After microwave treatment for 90 s, 13 GSLs in homogenized Chinese cabbage samples were recovered at 73–124%. However, a limitation of this method was that different tissues could not be processed under the same microwave conditions. Regarding universality, GSLs in Brassicaceae vegetables could be extracted from freeze-dried sample powder with 70% methanol (v/v) or frozen-fresh sample powder with 80% methanol (v/v). Moreover, heating extraction is necessary for GSLs extracted from frozen-fresh sample powder. Average recoveries of the two optimized methods were 74–119% with relative standard deviations ≤15%, with the limits of quantification 5.72–17.40 nmol/g dry weight and 0.80–1.43 nmol/g fresh weight, respectively. Notably, the method for analyzing intact GSLs was more efficient than that for desulfo-GSLs regarding operational complexity, detection speed and quantification accuracy. The developed method was applied to identify the characteristic GSLs in 15 Brassicaceae vegetables, providing a foundation for further research on GSLs.

Enzyme-Linked Immunosorbent Assay of Ochratoxin A in Barley

1983 ◽  
Vol 66 (6) ◽  
pp. 1481-1484 ◽  
Author(s):  
Michael R A Morgan ◽  
Ruth Mcnerney ◽  
Henry W-S Chan
Keyword(s):  
Sample Preparation ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Dilution ◽  
Assay Sensitivity ◽  
Double Antibody ◽  
Minimal Sample ◽  
Ochratoxin Α

Abstract A noncompetitive, double antibody enzyme-linked immunosorbent assay for ochratoxin A using microtitration plates has been developed and applied to samples of barley. The anti-ochratoxin A antiserum, which is used at high dilution, does not cross-react significantly with ochratoxin B or ochratoxin α, Assay sensitivity for determination of the toxin in barley samples is 60 ng/kg. Minimal sample preparation is required before assay.

Identifying tephras by alpha counting

1982 ◽  
Vol 19 (4) ◽  
pp. 662-665 ◽  
Author(s):  
A. B. Cormie ◽  
D. J. Huntley ◽  
D. E. Nelson
Keyword(s):  
North America ◽  
Sample Preparation ◽  
Field Technique ◽  
Minimal Sample

Alpha counting, as used for determination of uranium and thorium concentrations, was found useful for identifying some tephras found in northwest North America. The method has the advantage of requiring only minimal sample preparation and the potential of providing a field technique for identifying tephras.

A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

1987 ◽  
Vol 26 (01) ◽  
pp. 1-6 ◽  
Author(s):  
S. Selvaraj ◽  
M. R. Suresh ◽  
G. McLean ◽  
D. Willans ◽  
C. Turner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
T Antigen ◽  
Human Carcinomas ◽  
Animal Tumor ◽  
Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

Recent Trends in the Development of Green Microextraction Techniques for the Determination of Hazardous Organic Compounds in Wine

2019 ◽  
Vol 15 (7) ◽  
pp. 788-800 ◽  
Author(s):  
Natasa P. Kalogiouri ◽  
Victoria F. Samanidou
Keyword(s):  
Sample Preparation ◽  
Solid Phase Extraction ◽  
Organic Compounds ◽  
Method Development ◽  
Solid Phase ◽  
Stir Bar Sorptive Extraction ◽  
Phase Extraction ◽  
Microextraction Techniques ◽  
Hazardous Organic Compounds

Background:The sample preparation is the most crucial step in the analytical method development. Taking this into account, it is easily understood why the domain of sample preparation prior to detection is rapidly developing. Following the modern trends towards the automation, miniaturization, simplification and minimization of organic solvents and sample volumes, green microextraction techniques witness rapid growth in the field of food quality and safety. In a globalized market, it is essential to face the consumers need and develop analytical methods that guarantee the quality of food products and beverages. The strive for the accurate determination of organic hazards in a famous and appreciated alcoholic beverage like wine has necessitated the development of microextraction techniques.Objective:The objective of this review is to summarize all the recent microextraction methodologies, including solid phase extraction (SPE), solid phase microextraction (SPME), liquid-phase microextraction (LPME), dispersive liquid-liquid microextraction (DLLME), stir bar sorptive extraction (SBSE), matrix solid-phase dispersion (MSPD), single-drop microextraction (SDME) and dispersive solid phase extraction (DSPE) that were developed for the determination of hazardous organic compounds (pesticides, mycotoxins, colorants, biogenic amines, off-flavors) in wine. The analytical performance of the techniques is evaluated and their advantages and limitations are discussed.Conclusion:An extensive investigation of these techniques remains vital through the development of novel strategies and the implication of new materials that could upgrade the selectivity for the extraction of target analytes.

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