scholarly journals 3D-printed synthetic tissues

2016 ◽  
Vol 38 (4) ◽  
pp. 16-19 ◽  
Author(s):  
Michael J. Booth ◽  
Hagan Bayley
Keyword(s):  
Protein Synthesis ◽  
Synthetic Biology ◽  
Medical Applications ◽  
3D Printer ◽  
Bottom Up ◽  
Electrical Signals ◽  
Synthetic Cells ◽  
3D Printed ◽  
Living Tissues

‘Bottom-up’ approaches in synthetic biology have been used to construct synthetic cells from simple biological components. By contrast, relatively little work has been done on synthetic tissues in which collections of cells cooperate to achieve functionality that cannot be generated by individual compartments. We have developed a 3D printer, which can create structures containing hundreds or thousands of communicating aqueous droplets arranged in programmed patterns. These tissue-like materials can adopt properties such as the ability to fold or conduct electrical signals. Furthermore, the properties of the materials can be extended, so that they become true synthetic tissues through the performance of sophisticated functions such as protein synthesis. In addition, we have shown that 3D-printed synthetic tissues can be controlled and energized externally, for example by light. Printed synthetic tissues might find a variety of uses in medicine and could even be interfaced directly with living tissues. As they contain no genome and cannot replicate, synthetic tissues are comparatively safe for medical applications.

scholarly journals Synthetic tissues

2019 ◽  
Vol 3 (5) ◽  
pp. 615-622 ◽  
Author(s):  
Hagan Bayley ◽  
Idil Cazimoglu ◽  
Charlotte E.G. Hoskin
Keyword(s):  
Controlled Release ◽  
Three Dimensional ◽  
Therapeutic Agents ◽  
Hybrid Structures ◽  
Bottom Up ◽  
Synthetic Cells ◽  
3D Printed ◽  
Natural Living ◽  
Printed Materials ◽  
Living Tissues

While significant advances have been achieved with non-living synthetic cells built from the bottom-up, less progress has been made with the fabrication of synthetic tissues built from such cells. Synthetic tissues comprise patterned three-dimensional (3D) collections of communicating compartments. They can include both biological and synthetic parts and may incorporate features that do more than merely mimic nature. 3D-printed materials based on droplet-interface bilayers are the basis of the most advanced synthetic tissues and are being developed for several applications, including the controlled release of therapeutic agents and the repair of damaged organs. Current goals include the ability to manipulate synthetic tissues by remote signaling and the formation of hybrid structures with fabricated or natural living tissues.

scholarly journals pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells

2021 ◽  
Author(s):  
Felix Lussier ◽  
Martin Schroeter ◽  
Nicolas J Diercks ◽  
Kevin Jahnke ◽  
Cornelia Weber ◽  
...  
Keyword(s):  
Synthetic Biology ◽  
Current Status ◽  
Cellular Phenotype ◽  
Endomembrane System ◽  
Unilamellar Vesicles ◽  
Bottom Up ◽  
Cellular Processes ◽  
Simultaneous Reconstruction ◽  
Synthetic Cells ◽  
Small Unilamellar Vesicles

Bottom-up synthetic biology thrives to reconstruct basic cellular processes into a minimalist cellular replica to foster their investigation in greater details with a reduced number of variables. Among these cellular features, the endomembrane system is an important aspect of cells which is at the origin of many of their functions. Still, the reconstruction of these inner compartments within a lipid-based vesicle remains challenging and poorly controlled. Herein, we report the use of pH as external trigger to self-assemble compartmentalized giant unilamellar vesicles (GUVs) by either bulk, or droplet-based microfluidics. By co-encapsulating pH sensitive small unilamellar vesicles (SUVs), negatively charged SUVs and/or proteins, we show that acidification of the droplets efficiently produces GUVs while sequestrating the co-encapsulated material with flexibility and robustness. The method enables the simultaneous reconstruction of more than a single cellular phenotype from the bottom-up, corresponding to an important advancement in the current status quo of bottom-up synthetic biology.

scholarly journals Is Research on “Synthetic Cells” Moving to the Next Level?

Life ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 3 ◽  
Author(s):  
Pasquale Stano
Keyword(s):  
Gene Expression ◽  
Synthetic Biology ◽  
Rapid Expansion ◽  
Enzymatic Reactions ◽  
Bottom Up ◽  
Is Research ◽  
Synthetic Cells ◽  
Recent Trends

“Synthetic cells” research focuses on the construction of cell-like models by using solute-filled artificial microcompartments with a biomimetic structure. In recent years this bottom-up synthetic biology area has considerably progressed, and the field is currently experiencing a rapid expansion. Here we summarize some technical and theoretical aspects of synthetic cells based on gene expression and other enzymatic reactions inside liposomes, and comment on the most recent trends. Such a tour will be an occasion for asking whether times are ripe for a sort of qualitative jump toward novel SC prototypes: is research on “synthetic cells” moving to a next level?

scholarly journals Experimental studies on 3D printing of barium titanate ceramics for medical applications

2016 ◽  
Vol 2 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Mark Schult ◽  
Eric Buckow ◽  
Hermann Seitz
Keyword(s):  
Tissue Engineering ◽  
Barium Titanate ◽  
3D Printing ◽  
Piezoelectric Properties ◽  
Experimental Studies ◽  
Raw Material ◽  
Medical Applications ◽  
3D Printer ◽  
3D Printed ◽  
Titanate Ceramics

AbstractThe present work deals with the 3D printing of porous barium titanate ceramics. Barium titanate is a biocompatible material with piezoelectric properties. Due to insufficient flowability of the starting material for 3D printing, the barium titanate raw material has been modified in three different ways. Firstly, barium titanate powder has been calcined. Secondly, flow additives have been added to the powder. And thirdly, flow additives have been added to the calcined powder. Finally, a polymer has been added to the three materials and specimens have been printed from these three material mixtures. The 3D printed parts were then sintered at 1320°C. The sintering leads to shrinkage which differs between 29.51–71.53% for the tested material mixtures. The porosity of the parts is beneficial for cell growth which is relevant for future medical applications. The results reported in this study demonstrate the possibility to fabricate porous piezoelectric barium titanate parts with a 3D printer that can be used for medical applications. 3D printed porous barium titanate ceramics can especially be used as scaffold for bone tissue engineering, where the bone formation can be promoted by electrical stimulation.

scholarly journals Synthetic Cells Engaged in Molecular Communication: An Opportunity for Modelling Shannon- and Semantic-Information in the Chemical Domain

2021 ◽  
Vol 2 ◽  
Author(s):  
Maurizio Magarini ◽  
Pasquale Stano
Keyword(s):  
Synthetic Biology ◽  
Semantic Information ◽  
Emerging Technology ◽  
Shannon Information ◽  
Molecular Communication ◽  
Bottom Up ◽  
Nano Scale ◽  
Synthetic Cell ◽  
Synthetic Cells ◽  
Cell Fabrication

In this Perspective article we intend to focus on the opportunity of modelling Shannon information and/or “semantic” information in the field originated by the convergence of bottom-up synthetic biology (in particular, the construction of “synthetic cells”) and the engineering approaches to molecular communication. In particular we will argue that the emerging technology of synthetic cell fabrication will allow novel opportunities to study nano-scale communication and manipulation of information in unprecedented manner. More specifically, we will discuss the possibility of enquiring on the transfer and manipulation of information in the chemical domain, and interpreting such a dynamics according to Shannon or to MacKay-Bateson (“semantic” information).

scholarly journals Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40588-40596
Author(s):  
Tony Köhler ◽  
Thomas Heida ◽  
Sandra Hoefgen ◽  
Niclas Weigel ◽  
Vito Valiante ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Genetic Information ◽  
In Situ Immobilization ◽  
Bottom Up ◽  
Free Protein ◽  
Malonyl Coa ◽  
Cell Free Protein Synthesis

We describe a bottom-up approach towards functional enzymes utilizing microgels as carriers for genetic information that enable cell-free protein synthesis, in situ immobilization, and utilization of functional deGFP-MatB.

scholarly journals Design and characterization of 3D printed, neomycin-eluting poly-L-lactide mats for wound-healing applications

2021 ◽  
Vol 32 (4) ◽  
Author(s):  
Mahima Singh ◽  
Sriramakamal Jonnalagadda
Keyword(s):  
Release Kinetics ◽  
Biological Properties ◽  
In Vitro Dissolution ◽  
3D Printer ◽  
Topical Antibiotic ◽  
Young’S Moduli ◽  
Base Polymer ◽  
Potential Applications ◽  
3D Printed

AbstractThis study evaluates the suitability of 3D printed biodegradable mats to load and deliver the topical antibiotic, neomycin, for up to 3 weeks in vitro. A 3D printer equipped with a hot melt extruder was used to print bandage-like wound coverings with porous sizes appropriate for cellular attachment and viability. The semicrystalline polyester, poly-l-lactic acid (PLLA) was used as the base polymer, coated (post-printing) with polyethylene glycols (PEGs) of MWs 400 Da, 6 kDa, or 20 kDa to enable manipulation of physicochemical and biological properties to suit intended applications. The mats were further loaded with a topical antibiotic (neomycin sulfate), and cumulative drug-release monitored for 3 weeks in vitro. Microscopic imaging as well as Scanning Electron Microscopy (SEM) studies showed pore dimensions of 100 × 400 µm. These pore dimensions were achieved without compromising mechanical strength; because of the “tough” individual fibers constituting the mat (Young’s Moduli of 50 ± 20 MPa and Elastic Elongation of 10 ± 5%). The in vitro dissolution study showed first-order release kinetics for neomycin during the first 20 h, followed by diffusion-controlled (Fickian) release for the remaining duration of the study. The release of neomycin suggested that the ability to load neomycin on to PLLA mats increases threefold, as the MW of the applied PEG coating is lowered from 20 kDa to 400 Da. Overall, this study demonstrates a successful approach to using a 3D printer to prepare porous degradable mats for antibiotic delivery with potential applications to dermal regeneration and tissue engineering.

Mechatronic Design of ARC Humanoid Robot Open Platform: First Fully 3D Printed Kid-Sized Robot

2020 ◽  
Vol 17 (03) ◽  
pp. 2050010
Author(s):  
Saeed Saeedvand ◽  
Hadi S. Aghdasi ◽  
Jacky Baltes
Keyword(s):  
Humanoid Robot ◽  
Size Range ◽  
Robot Design ◽  
3D Printer ◽  
Wide Angle ◽  
Open Platform ◽  
3D Printed ◽  
Wide Angle Camera ◽  
Computational Resources ◽  
User Friendly

Although there are several popular and capable humanoid robot designs available in the kid-size range, they lack some important characteristics: affordability, being user-friendly, using a wide-angle camera, sufficient computational resources for advanced AI algorithms, and mechanical robustness and stability are the most important ones. Recent advances in 3D printer technology enables researchers to move from model to physical implementation relatively easy. Therefore, we introduce a novel fully 3D printed open platform humanoid robot design named ARC. In this paper, we discuss the mechanical structure and software architecture. We show the capabilities of the ARC design in a series of experimental evaluations.

Preliminary Study of Alginates Extracted from Brown Algae (Sargassum sp.) Available in Madura Island as Composite Based Hydrogel Materials

2019 ◽  
Vol 964 ◽  
pp. 240-245 ◽  
Author(s):  
Amaliya Rasyida ◽  
Thalyta Rizkha Pradipta ◽  
Sigit Tri Wicaksono ◽  
Vania Mitha Pratiwi ◽  
Yeny Widya Rakhmawati
Keyword(s):  
Sodium Alginate ◽  
Brown Algae ◽  
Extraction Process ◽  
Composition Analysis ◽  
3D Printer ◽  
X Ray Diffraction ◽  
X Ray ◽  
3D Printed ◽  
Preliminary Study

Utilization of brown algae especially in Madura, where it’s close to Surabaya, only limited for food. This become a reference for developing and increasing the potential of this algae by extracting one of the ingredients, namely alginate. This paper deals with the characterization of sodium alginate extracted from sargassum sp. using modified-purified calcium routes. The extracted sodium alginate will be further used as composite hydrogel materials and compared with commercial sodium alginate. Hereafter, the synthesized composite is expected to be bio-ink for 3d printer. Chemical composition analysis were analyzed using X-Ray Fluorosense (XRF) followed by Fourier-transform infrared spectroscopy (FTIR) analysis to identify the functional group of composite and X-Ray Diffraction (XRD). Furthermore, viscosity bath is performed to compare the viscosity of extracted and commercial one. The result shows that modified-purified calcium routes in the extraction process of sodium alginate is desirable for improving their properties. Interestingly enough, with the goal of using it as bio-ink in 3d printed fabrication, the synthesized composite shows viscosity, 300 cSt, which meets the criteria for bio-ink in 3d printer.

Evolving protocells to prototissues: rational design of a missing link

2013 ◽  
Vol 41 (5) ◽  
pp. 1159-1165 ◽  
Author(s):  
Shiksha Mantri ◽  
K. Tanuj Sapra
Keyword(s):  
Synthetic Biology ◽  
Rational Design ◽  
Structural Elements ◽  
Bottom Up ◽  
Missing Link ◽  
Hierarchical Assembly ◽  
Artificial Cell

Realization of a functional artificial cell, the so-called protocell, is a major challenge posed by synthetic biology. A subsequent goal is to use the protocellular units for the bottom-up assembly of prototissues. There is, however, a looming chasm in our knowledge between protocells and prototissues. In the present paper, we give a brief overview of the work on protocells to date, followed by a discussion on the rational design of key structural elements specific to linking two protocellular bilayers. We propose that designing synthetic parts capable of simultaneous insertion into two bilayers may be crucial in the hierarchical assembly of protocells into a functional prototissue.

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