Characterization of a cry4Ba-type gene of Bacillus thuringiensis israelensis and evidence of the synergistic larvicidal activity of its encoded protein with Cry2A δ-endotoxin of B. thuringiensis kurstaki on Culex pipiens (common house mosquito)

2006 ◽  
Vol 44 (1) ◽  
pp. 19 ◽  
Author(s):  
Slim Tounsi ◽  
Raida Zribi Zghal ◽  
Samir Jaoua
Keyword(s):  
Bacillus Thuringiensis ◽  
Larvicidal Activity ◽  
Culex Pipiens ◽  
Bacillus Thuringiensis Israelensis ◽  
Type Gene ◽  
Common House

Nucleotide sequence and characterization of a new insertion element, IS240, from Bacillus thuringiensis israelensis

Plasmid ◽  
1989 ◽  
Vol 21 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Armelle Delecluse ◽  
Catherine Bourgouin ◽  
Andre Klier ◽  
Georges Rapoport
Keyword(s):  
Bacillus Thuringiensis ◽  
Nucleotide Sequence ◽  
Insertion Element ◽  
Bacillus Thuringiensis Israelensis

scholarly journals Microbiota and transcriptome changes of Culex pipiens pallens larvae exposed to Bacillus thuringiensis israelensis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ruiling Zhang ◽  
Wenjuan Liu ◽  
Qian Zhang ◽  
Xinyu Zhang ◽  
Zhong Zhang
Keyword(s):  
Bacillus Thuringiensis ◽  
Culex Pipiens ◽  
Mosquito Control ◽  
Sphingolipid Metabolism ◽  
Glutathione Metabolism ◽  
Control Group ◽  
Culex Pipiens Pallens ◽  
Bacillus Thuringiensis Israelensis ◽  
Epidemic Encephalitis ◽  
Pipiens Pallens

AbstractCulex pipiens pallens is an important vector of lymphatic filariasis and epidemic encephalitis. Mosquito control is the main strategy used for the prevention of mosquito-borne diseases. Bacillus thuringiensis israelensis (Bti) is an entomopathogenic bacterium widely used in mosquito control. In this study, we profiled the microbiota and transcriptional response of the larvae of Cx. pipiens pallens exposed to different concentrations of Bti. The results demonstrated that Bti induced a significant effect on both the microbiota and gene expression of Cx. pipiens pallens. Compared to the control group, the predominant bacteria changed from Actinobacteria to Firmicutes, and with increase in the concentration of Bti, the abundance of Actinobacteria was gradually reduced. Similar changes were also detected at the genus level, where Bacillus replaced Microbacterium, becoming the predominant genus in Bti-exposed groups. Furthermore, alpha diversity analysis indicated that Bti exposure changed the diversity of the microbota, possibly because the dysbiosis caused by the Bti infection inhibits some bacteria and provides opportunities to other opportunistic taxa. Pathway analysis revealed significant enhancement for processes associated with sphingolipid metabolism, glutathione metabolism and glycerophospholipid metabolism between all Bti-exposed groups and control group. Additionally, genes associated with the Toll and Imd signaling pathway were found to be notably upregulated. Bti infection significantly changed the bacterial community of larvae of Cx. pipiens pallens.

An efficient gene deletion system for Bacillus thuringiensis

Biologia ◽  
2013 ◽  
Vol 68 (3) ◽  
Author(s):  
Tugrul Doruk ◽  
Sedef Gedik
Keyword(s):  
Bacillus Thuringiensis ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Bacillus Thuringiensis Israelensis ◽  
Kinase Gene ◽  
E Coli ◽  
Efficient Gene ◽  
Specific Restriction ◽  
Type Gene ◽  
Deletion Cassette

AbstractIt is not easy to manipulate biosynthetic genes of Bacillus thuringiensis since there is a powerful methyl-specific restriction system in this microorganism. In this study, a PCR-based system was used to delete polyphosphate kinase gene (ppk) of Bacillus thuringiensis israelensis (Bti) by replacing the wild-type gene with a cassette containing the apramycin resistance gene as selectable marker. λ-Red was used to promote recombination in Escherichia coli between a PCR-amplified apramycin resistance cassette (linear deletion cassette selectable in E. coli and Bti) and Bti DNA on a plasmid. The isolated mutant plasmid was transferred to Bti by conjugation. Double cross-over transformants were screened for their antibiotic resistance and the mutation was proven by PCR, southern blot hybridization and RT-PCR. The described method, which uses the advantage of quick plasmid construction in E. coli and simple transformation of linear deletion cassette, is very useful to delete entire gene/genes of Bti without any polar effects on genes transcriptionally downstream.

Isolation and Characterization of a Bacillus thuringiensis ssp. kurstaki Strain Toxic to Spodoptera exigua and Culex pipiens

2001 ◽  
Vol 43 (4) ◽  
pp. 284-287 ◽  
Author(s):  
I.H. Lee ◽  
Y.H. Je ◽  
J.H. Chang ◽  
J.Y. Roh ◽  
H.W. Oh ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Culex Pipiens ◽  
Spodoptera Exigua ◽  
Isolation And Characterization

scholarly journals Identification of vip3A-type genes from Bacillus thuringiensis strains and characterization of a novel vip3A-type gene

2007 ◽  
Vol 45 (4) ◽  
pp. 432-438 ◽  
Author(s):  
J. Liu ◽  
F. Song ◽  
J. Zhang ◽  
R. Liu ◽  
K. He ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Type Gene
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