Molecular cloning and characterization of a novel vip3-type gene from Bacillus thuringiensis and evaluation of its toxicity against Helicoverpa armigera

2018 ◽  
Vol 114 ◽  
pp. 464-469 ◽  
Author(s):  
Showkat Ahmad Lone ◽  
Abdul Malik ◽  
Jasdeep Chatrath Padaria
Keyword(s):  
Bacillus Thuringiensis ◽  
Molecular Cloning ◽  
Helicoverpa Armigera ◽  
Helicoverpa Armígera ◽  
Type Gene

Characterization of Bacillus thuringiensis isolates and their differential toxicity against Helicoverpa armigera populations

2010 ◽  
Vol 51 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Deepak Anitha ◽  
Nachimuthu Senthil Kumar ◽  
Deepu Vijayan ◽  
Kunhikrishnan Ajithkumar ◽  
Guruswami Gurusubramanian
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Differential Toxicity ◽  
Helicoverpa Armígera

Molecular cloning and characterization of a C-type lectin from the cotton bollworm, Helicoverpa armigera

2008 ◽  
Vol 32 (1) ◽  
pp. 71-83 ◽  
Author(s):  
Lian-Qin Chai ◽  
Yuan-Yuan Tian ◽  
Dan-Tong Yang ◽  
Jin-Xing Wang ◽  
Xiao-Fan Zhao
Keyword(s):  
Molecular Cloning ◽  
Helicoverpa Armigera ◽  
Cotton Bollworm ◽  
Helicoverpa Armígera

Molecular cloning and characterization of Hearm caspase-1 from Helicoverpa armigera

2007 ◽  
Vol 35 (3) ◽  
pp. 405-412 ◽  
Author(s):  
Dantong Yang ◽  
Lianqin Chai ◽  
Jinxing Wang ◽  
Xiaofan Zhao
Keyword(s):  
Molecular Cloning ◽  
Helicoverpa Armigera ◽  
Caspase 1 ◽  
Helicoverpa Armígera

Characterization of Helicoverpa armigera spatial distribution in pigeonpea crop using geostatistical methods

2021 ◽  
Author(s):  
S. Malathi ◽  
B. Kiran Gandhi ◽  
Murari Kumar ◽  
Shabistana Nisa ◽  
Puran Chandra ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Helicoverpa Armigera ◽  
Geostatistical Methods ◽  
Helicoverpa Armígera

scholarly journals Bacillus thuringiensis Bel Protein Enhances the Toxicity of Cry1Ac Protein to Helicoverpa armigera Larvae by Degrading Insect Intestinal Mucin

2009 ◽  
Vol 75 (16) ◽  
pp. 5237-5243 ◽  
Author(s):  
Shangling Fang ◽  
Li Wang ◽  
Wei Guo ◽  
Xia Zhang ◽  
Donghai Peng ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Trichoplusia Ni ◽  
Knockout Mutant ◽  
Gene Encoding ◽  
In Vitro Degradation ◽  
Intestinal Mucin ◽  
Helicoverpa Armígera ◽  
Cry1ac Protein

ABSTRACT Bacillus thuringiensis has been used as a bioinsecticide to control agricultural insects. Bacillus cereus group genomes were found to have a Bacillus enhancin-like (bel) gene, encoding a peptide with 20 to 30% identity to viral enhancin protein, which can enhance viral infection by degradation of the peritrophic matrix (PM) of the insect midgut. In this study, the bel gene was found to have an activity similar to that of the viral enhancin gene. A bel knockout mutant was constructed by using a plasmid-free B. thuringiensis derivative, BMB171. The 50% lethal concentrations of this mutant plus the cry1Ac insecticidal protein gene were about 5.8-fold higher than those of the BMB171 strain. When purified Bel was mixed with the Cry1Ac protein and fed to Helicoverpa armigera larvae, 3 μg/ml Cry1Ac alone induced 34.2% mortality. Meanwhile, the mortality rate rose to 74.4% when the same amount of Cry1Ac was mixed with 0.8 μg/ml of Bel. Microscopic observation showed a significant disruption detected on the midgut PM of H. armigera larvae after they were fed Bel. In vitro degradation assays showed that Bel digested the intestinal mucin (IIM) of Trichoplusia ni and H. armigera larvae to various degrading products, similar to findings for viral enhancin. These results imply Bel toxicity enhancement depends on the destruction of midgut PM and IIM, similar to the case with viral enhancin. This discovery showed that Bel has the potential to enhance insecticidal activity of B. thuringiensis-based biopesticides and transgenic crops.

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