Triazinylaniline derivatives as fluorescence probes. Part 3. Effects of calcium and other metal ions on the steady-state and time-resolved fluorescence of bovine brain calmodulin labelled at lysine-75

Author(s):  
David J. Cowley ◽  
James P. McCormick
2010 ◽  
Vol 8 (3) ◽  
pp. 674-686 ◽  
Author(s):  
Magda Milewska ◽  
Katarzyna Guzow ◽  
Wiesław Wiczk

AbstractThe ability of new chelate ligands, benzoxazol-5-yl-alanine derivatives substituted in position 2 by heteroaromatic substituent, to form complexes with selected metal ions in acetonitrile are studied by means of absorption and steady-state and time-resolved fluorescence spectroscopy. Among the ligands studied, only azaaromatic derivatives form stable complexes with transition metal ions in the ground state. Their absorption bands are bathochromically shifted enabling to use those ligands as ratiometric sensors. The fluorescence of each ligand is quenched by metal ions, however, in the presence of Cd(II) and Zn(II) ions a new red shifted emission band is observed.


2018 ◽  
Vol 20 (21) ◽  
pp. 14796-14807 ◽  
Author(s):  
Soumya Kanti De ◽  
Nishu Kanwa ◽  
Mirajuddin Ahamed ◽  
Anjan Chakraborty

In this manuscript, we investigate the interactions of different metal ions with zwitterionic phospholipid bilayers of different chain lengths using the well-known membrane probe PRODAN and steady state and time resolved fluorescence spectroscopy.


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