Steady-state fluorescence and time-resolved fluorescence monitor changes in tryptophan environment in arginase from Saccharomyces cerevisiae upon removal of catalytic and structural metal ions

Biochemistry ◽  
1990 ◽  
Vol 29 (39) ◽  
pp. 9159-9168 ◽  
Author(s):  
Susan M. Green ◽  
Jay R. Knutson ◽  
Preston Hensley
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Steady State ◽  
Metal Ions ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Steady State Fluorescence ◽  
Structural Metal

scholarly journals Fluorescent chemosensors for metal ions based on 3-(2-benzoxazol-5-yl)alanine skeleton

2010 ◽  
Vol 8 (3) ◽  
pp. 674-686 ◽  
Author(s):  
Magda Milewska ◽  
Katarzyna Guzow ◽  
Wiesław Wiczk
Keyword(s):  
Steady State ◽  
Fluorescence Spectroscopy ◽  
Metal Ions ◽  
Ground State ◽  
Transition Metal Ions ◽  
Fluorescent Chemosensors ◽  
Time Resolved Fluorescence ◽  
Absorption Bands ◽  
Time Resolved ◽  
Ratiometric Sensors

AbstractThe ability of new chelate ligands, benzoxazol-5-yl-alanine derivatives substituted in position 2 by heteroaromatic substituent, to form complexes with selected metal ions in acetonitrile are studied by means of absorption and steady-state and time-resolved fluorescence spectroscopy. Among the ligands studied, only azaaromatic derivatives form stable complexes with transition metal ions in the ground state. Their absorption bands are bathochromically shifted enabling to use those ligands as ratiometric sensors. The fluorescence of each ligand is quenched by metal ions, however, in the presence of Cd(II) and Zn(II) ions a new red shifted emission band is observed.

Spectroscopic evidence for hydration and dehydration of lipid bilayers upon interaction with metal ions: a new physical insight

2018 ◽  
Vol 20 (21) ◽  
pp. 14796-14807 ◽  
Author(s):  
Soumya Kanti De ◽  
Nishu Kanwa ◽  
Mirajuddin Ahamed ◽  
Anjan Chakraborty
Keyword(s):  
Steady State ◽  
Metal Ions ◽  
Lipid Bilayers ◽  
Phospholipid Bilayers ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Membrane Probe ◽  
Physical Insight ◽  
Chain Lengths ◽  
Hydration And Dehydration

In this manuscript, we investigate the interactions of different metal ions with zwitterionic phospholipid bilayers of different chain lengths using the well-known membrane probe PRODAN and steady state and time resolved fluorescence spectroscopy.

scholarly journals Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)

2013 ◽  
Vol 85 (3) ◽  
pp. 589-608 ◽  
Author(s):  
Marcel Ameloot ◽  
Martin vandeVen ◽  
A. Ulises Acuña ◽  
Bernard Valeur
Keyword(s):  
Steady State ◽  
Fluorescence Anisotropy ◽  
Fluorescence Polarization ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Steady State Fluorescence ◽  
Technical Report ◽  
Accuracy Of Measurements ◽  
Solution Methods ◽  
Systematic Effects

After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.

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