Constituents of Withania somnifera Dun. Part VIII. A new steroidal lactone, 27-deoxy-14-hydroxy-withaferin A

1966 ◽  
pp. 1765 ◽  
Author(s):  
E. Glotter ◽  
R. Waitman ◽  
D. Lavie
Keyword(s):  
Withania Somnifera ◽  
Withaferin A ◽  
Steroidal Lactone

scholarly journals Withaferin A – A natural multifaceted therapeutic compound

2021 ◽  
pp. 36-52
Author(s):  
Sangeetha Vinod ◽  
Kalaiselvi Senthil
Keyword(s):  
Withania Somnifera ◽  
Plant Biomass ◽  
Wide Spectrum ◽  
Withaferin A ◽  
Drug Candidate ◽  
Global Pandemic ◽  
Medicinal Properties ◽  
Therapeutic Compound ◽  
Steroidal Lactone

COVID 19, which has lead to the death of millions of people, is still spreading worldwide. The development of any new drug after proper trial is much time consuming.  This present global pandemic situation has lead the researchers around the world to run behind various existing antiviral and immunomodulatory natural compounds to overcome this contagious disease.  Withania somnifera (ashwagandha) that is being used in Ayurvedic medicine for several ailments since several years is also said to pocess anti viral activity. Thus many of its metabolites are being studied individually for its efficacy against the dreadful disease. Withaferin A, a steroidal lactone from ashwagandha is one such prime metabolite which beyond acting as an antioxidant or antimicrobial agent, is also said to pocess anti-inflammatory to anti carcinogenic properties.  Thus because of its wide spectrum of medicinal properties it has now become an attractive drug candidate for several preclinical studies. This increase in the demand for withaferin A has chanelled its way towards in vitro propagation of the plant Withania somnifera and trials on various strategies to increase the yield in terms of plant biomass as well as the withaferin content in the plants thus making it a better alternative to field grown plants. Thus this article reviews in depth on the important medicinal properties of withaferin A, its demand in Ayurveda industry and the in vitro strategies being carried out to overcome the demand.

scholarly journals Pharmacological Levels of Withaferin A (Withania somnifera) Trigger Clinically Relevant Anticancer Effects Specific to Triple Negative Breast Cancer Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e87850 ◽  
Author(s):  
Katarzyna Szarc vel Szic ◽  
Ken Op de Beeck ◽  
Dariusz Ratman ◽  
An Wouters ◽  
Ilse M. Beck ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Withania Somnifera ◽  
Triple Negative ◽  
Withaferin A ◽  
Anticancer Effects

Traditional Horticulture Practices Increase the Production of Selected Withanolides in Withania Somnifera (L.) Dunal—A RP-UFLC Analysis

2020 ◽  
Vol 58 (10) ◽  
pp. 899-906
Author(s):  
Gireesh M Ankad ◽  
Sandeep R Pai ◽  
Jagadishchandra Hiremath ◽  
Harsha V Hegde
Keyword(s):  
Withania Somnifera ◽  
Farmyard Manure ◽  
Simultaneous Detection ◽  
Withaferin A ◽  
Reverse Phase ◽  
Ancient India ◽  
Enhanced Production ◽  
Plant Life ◽  
Multiple Comparison Test ◽  
Withanolide A

Abstract The study evaluates the effect of two traditional horticulture treatments mentioned in Vrikshayurveda, a text from ancient India on the science of plant life, namely Kunapa jala (KJ) and Pancha gavya (PG) on the production of Withaferin A (WFA), withanolide A (WIA) and Withanolide B (WIB) in Withania somnifera (L) Dunal. Leaves and roots of W. somnifera were collected from different treated groups viz. control, KJ, PG, farmyard manure (FYM) and inorganic fertilizer (NPK). Reverse phase ultra-flow liquid chromatography (RP-UFLC) method was developed, validated for simultaneous detection of WFA, WIA and WIB. Statistical analysis of data was performed by ANOVA and tested for significance by the Dunnett multiple comparison test and data were expressed as mean ± standard deviation (SD). Results revealed, leaves possessed highest WFA content and roots possessed highest content of WIA and WIB. PG treated leaves were observed highest WFA (18.29 mg/g) and roots were observed highest WIA (19.63 mg/g) and WIB (1.36 mg/g). Conclusively, RP-UFLC method for simultaneous detection of withanolides has been developed and validated to evaluate the effect of traditional horticulture treatments. It is concluded that the enhanced production of withanolides can be achieved by the application of PG when compared to NPK application.

scholarly journals Neuroprotective Effect of CR-777, a Glutathione Derivative of Withaferin A, Obtained through the Bioconversion of Withania somnifera (L.) Dunal Extract by the Fungus Beauveria bassiana

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4599 ◽  
Author(s):  
Chérif Rabhi ◽  
Guillaume Arcile ◽  
Géraldine Le Goff ◽  
Christian Da Costa Noble ◽  
Jamal Ouazzani
Keyword(s):  
Beauveria Bassiana ◽  
Cortical Neurons ◽  
Withania Somnifera ◽  
Neuroprotective Effect ◽  
2D Nmr ◽  
Withaferin A ◽  
Protective Agent ◽  
Specific Staining ◽  
6 Hydroxydopamine ◽  
Fungus Beauveria Bassiana

The bioconversion of Withania somnifera extract by the fungus Beauveria bassiana leads to cysteine and glutathione derivatives of withaferin A at the C-6 position. The compounds were purified and fully characterized by 1D-NMR, 2D-NMR, and HRMS analysis. The glutathione derivative CR-777 was evaluated as a neuroprotective agent from damage caused by different neurotoxins mimicking molecular symptoms in Parkinson´s disease (PD), including 1-methyl-4-phenylpyridinium (MPP+), 6-hydroxydopamine (6-OHDA), and α-synuclein (α-Syn). CR-777, at nanomolar concentrations, protected dopaminergic and cortical neurons. In 6-OHDA-treated neurons, CR-777 increased cell survival and neurite network and decreased the expression of α-Syn. Using specific inhibitors of cell toxicity signaling pathways and specific staining experiments, the observed role of CR-777 seemed to involve the PI3K/mTOR pathway. CR-777 could be considered as a protective agent against a large panel of neuronal stressors and was engaged in further therapeutic development steps.

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