Enhanced Anti-tumor Activity of Drug through pH-Triggered Release and Dual Targeting by Calcium Phosphate-Covered Mesoporous Silica Vehicles

2021 ◽  
Author(s):  
Juan Liu ◽  
Xixue Hu ◽  
Shubin Jin ◽  
Xing-Jie Liang ◽  
Xiaowei Ma
Keyword(s):  
Controlled Release ◽  
Calcium Phosphate ◽  
Targeted Delivery ◽  
Antitumor Drugs ◽  
Triggered Release ◽  
Dual Targeting ◽  
Rapid Release ◽  
Main Factors ◽  
Anti Tumor Activity

Rapid release and clearance of antitumor drugs in vivo are the main factors to evade the effectiveness of chemotherapeutics. Targeted delivery and controlled release of drugs are the most pressing...

Combination of hybrid peptide with biodegradable gelatin hydrogel for controlled release and enhancement of anti-tumor activity in vivo

2014 ◽  
Vol 176 ◽  
pp. 1-7 ◽  
Author(s):  
Arong Gaowa ◽  
Tomohisa Horibe ◽  
Masayuki Kohno ◽  
Keisuke Sato ◽  
Hiroshi Harada ◽  
...  
Keyword(s):  
Controlled Release ◽  
Hybrid Peptide ◽  
Gelatin Hydrogel ◽  
Anti Tumor Activity

Magnetically controlled release of recombinant tissue plasminogen activator from chitosan nanocomposites for targeted thrombolysis

2016 ◽  
Vol 4 (15) ◽  
pp. 2578-2590 ◽  
Author(s):  
Jyh-Ping Chen ◽  
Chih-Hsin Liu ◽  
Hao-Lung Hsu ◽  
Tony Wu ◽  
Yu-Jen Lu ◽  
...  
Keyword(s):  
Controlled Release ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Targeted Delivery ◽  
Chitosan Nanoparticles ◽  
Recombinant Tissue Plasminogen Activator ◽  
Triggered Release ◽  
Magnetic Chitosan ◽  
Tissue Plasminogen ◽  
Thrombolysis Therapy

Targeted delivery and triggered release of rtPA-encapsulated magnetic chitosan nanoparticles with the guidance of a magnet could be used for remote-controlled thrombolysis therapy.

scholarly journals Multifunctional DNA Nanomaterials for Biomedical Applications

2015 ◽  
Vol 2015 ◽  
pp. 1-21 ◽  
Author(s):  
Dick Yan Tam ◽  
Pik Kwan Lo
Keyword(s):  
Targeted Delivery ◽  
Materials Science ◽  
Drug Loading ◽  
Dna Nanotechnology ◽  
Control Release ◽  
Structural Features ◽  
Cellular Systems ◽  
Triggered Release ◽  
Self Assembled

The rapidly emerging DNA nanotechnology began with pioneer Seeman’s hypothesis that DNA not only can carry genetic information but also can be used as molecular organizer to create well-designed and controllable nanomaterials for applications in materials science, nanotechnology, and biology. DNA-based self-assembly represents a versatile system for nanoscale construction due to the well-characterized conformation of DNA and its predictability in the formation of base pairs. The structural features of nucleic acids form the basis of constructing a wide variety of DNA nanoarchitectures with well-defined shapes and sizes, in addition to controllable permeability and flexibility. More importantly, self-assembled DNA nanostructures can be easily functionalized to construct artificial functional systems with nanometer scale precision for multipurposes. Apparently scientists envision artificial DNA-based nanostructures as tool for drug loading andin vivotargeted delivery because of their abilities in selective encapsulation and stimuli-triggered release of cargo. Herein, we summarize the strategies of creating multidimensional self-assembled DNA nanoarchitectures and review studies investigating their stability, toxicity, delivery efficiency, loading, and control release of cargos in addition to their site-specific targeting and delivery of drug or cargo molecules to cellular systems.

scholarly journals Targeted Delivery of Narrow-Spectrum Protein Antibiotics to the Lower Gastrointestinal Tract in a Murine Model of Escherichia coli Colonization

2021 ◽  
Vol 12 ◽  
Author(s):  
Nuria Carpena ◽  
Kerry Richards ◽  
Teresita D. J. Bello Gonzalez ◽  
Alberto Bravo-Blas ◽  
Nicholas G. Housden ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Delivery ◽  
Controlled Release ◽  
Animal Models ◽  
Targeted Delivery ◽  
Intestinal Colonization ◽  
Ph Responsive ◽  
Human Gut ◽  
E Coli

Bacteriocins are narrow-spectrum protein antibiotics that could potentially be used to engineer the human gut microbiota. However, technologies for targeted delivery of proteins to the lower gastrointestinal (GI) tract in preclinical animal models are currently lacking. In this work, we have developed methods for the microencapsulation of Escherichia coli targeting bacteriocins, colicin E9 and Ia, in a pH responsive formulation to allow their targeted delivery and controlled release in an in vivo murine model of E. coli colonization. Membrane emulsification was used to produce a water-in-oil emulsion with the water-soluble polymer subsequently cross-linked to produce hydrogel microcapsules. The microcapsule fabrication process allowed control of the size of the drug delivery system and a near 100% yield of the encapsulated therapeutic cargo. pH-triggered release of the encapsulated colicins was achieved using a widely available pH-responsive anionic copolymer in combination with alginate biopolymers. In vivo experiments using a murine E. coli intestinal colonization model demonstrated that oral delivery of the encapsulated colicins resulted in a significant decrease in intestinal colonization and reduction in E. coli shedding in the feces of the animals. Employing controlled release drug delivery systems such as that described here is essential to enable delivery of new protein therapeutics or other biological interventions for testing within small animal models of infection. Such approaches may have considerable value for the future development of strategies to engineer the human gut microbiota, which is central to health and disease.

Controlled release of rhBMP-2 loaded poly(dl-lactic-co-glycolic acid)/calcium phosphate cement composites in vivo

2005 ◽  
Vol 106 (1-2) ◽  
pp. 162-171 ◽  
Author(s):  
P.Q. Ruhé ◽  
O.C. Boerman ◽  
F.G.M. Russel ◽  
P.H.M. Spauwen ◽  
A.G. Mikos ◽  
...  
Keyword(s):  
Controlled Release ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Glycolic Acid ◽  
Cement Composites

scholarly journals A multistage assembly/disassembly strategy for tumor-targeted CO delivery

2020 ◽  
Vol 6 (20) ◽  
pp. eaba1362 ◽  
Author(s):  
Jin Meng ◽  
Zhaokui Jin ◽  
Penghe Zhao ◽  
Bin Zhao ◽  
Mingjian Fan ◽  
...  
Keyword(s):  
Controlled Release ◽  
Targeted Delivery ◽  
Tumor Tissue ◽  
Silica Nanoparticle ◽  
Tissue Cell ◽  
Anticancer Efficacy ◽  
Electrostatic Assembly ◽  
Gas Molecule

CO gas molecule not only could selectively kill cancer cells but also exhibits limited anticancer efficacy because of the lack of active tumor-targeted accumulation capability. In this work, a multistage assembly/disassembly strategy is developed to construct a new intelligent nanomedicine by encapsulating a mitochondria-targeted and intramitochondrial microenvironment–responsive prodrug (FeCO-TPP) within mesoporous silica nanoparticle that is further coated with hyaluronic acid by step-by-step electrostatic assembly, realizing tumor tissue–cell–mitochondria–targeted multistage delivery and controlled release of CO in a step-by-step disassembly way. Multistage targeted delivery and controlled release of CO involve (i) the passive tumor tissue–targeted nanomedicine delivery, (ii) the active tumor cell–targeted nanomedicine delivery, (iii) the acid-responsive prodrug release, (iv) the mitochondria-targeted prodrug delivery, and (v) the ROS-responsive CO release. The developed nanomedicine has effectively augmented the efficacy and safety of CO therapy of cancer both in vitro and in vivo. The proposed multistage assembly/disassembly strategy opens a new window for targeted CO therapy.

Controlled Release of rhBMP-2 Loaded Poly(DL-lactic-co-glycolic Acid)/ Calcium Phosphate Cement Composites In Vivo

2006 ◽  
pp. 973-976
Author(s):  
P.Q. Ruhé ◽  
O.C. Boerman ◽  
F.G.M. Russel ◽  
P.H.M. Spauwen ◽  
Antonious G. Mikos ◽  
...  
Keyword(s):  
Controlled Release ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Glycolic Acid ◽  
Cement Composites

Smart aptamer-modified calcium carbonate nanoparticles for controlled release and targeted delivery of epirubicin and melittin into cancer cells in vitro and in vivo

2019 ◽  
Vol 45 (4) ◽  
pp. 603-610 ◽  
Author(s):  
Rezvan Yazdian-Robati ◽  
Atefeh Arab ◽  
Mohammad Ramezani ◽  
Houshang Rafatpanah ◽  
Amirhossein Bahreyni ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Controlled Release ◽  
Cancer Cells ◽  
Targeted Delivery

Multifunctional halloysite nanotubes for targeted delivery and controlled release of doxorubicin in-vitro and in-vivo studies

2017 ◽  
Vol 28 (37) ◽  
pp. 375101 ◽  
Author(s):  
Yuwei Hu ◽  
Jian Chen ◽  
Xiufang Li ◽  
Yanhua Sun ◽  
Shen Huang ◽  
...  
Keyword(s):  
Controlled Release ◽  
Targeted Delivery ◽  
Halloysite Nanotubes ◽  
In Vivo Studies

scholarly journals In Vitro Release of Bioactive Bone Morphogenetic Proteins (GDF5, BB-1, and BMP-2) from a PLGA Fiber-Reinforced, Brushite-Forming Calcium Phosphate Cement

Pharmaceutics ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 455
Author(s):  
Francesca Gunnella ◽  
Elke Kunisch ◽  
Victoria Horbert ◽  
Stefan Maenz ◽  
Jörg Bossert ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Cell Lines ◽  
Bone Morphogenetic Proteins ◽  
Calcium Phosphate Cement ◽  
Targeted Delivery ◽  
Calf Serum ◽  
In Vivo Studies ◽  
Alp Activity

Bone regeneration of sheep lumbar osteopenia is promoted by targeted delivery of bone morphogenetic proteins (BMPs) via a biodegradable, brushite-forming calcium-phosphate-cement (CPC) with stabilizing poly(l-lactide-co-glycolide) acid (PLGA) fibers. The present study sought to quantify the release and bioactivity of BMPs from a specific own CPC formulation successfully used in previous in vivo studies. CPC solid bodies with PLGA fibers (0%, 5%, 10%) containing increasing dosages of GDF5, BB-1, and BMP-2 (2 to 1000 µg/mL) were ground and extracted in phosphate-buffered saline (PBS) or pure sheep serum/cell culture medium containing 10% fetal calf serum (FCS; up to 30/31 days). Released BMPs were quantified by ELISA, bioactivity was determined via alkaline phosphatase (ALP) activity after 3-day exposure of different osteogenic cell lines (C2C12; C2C12BRlb with overexpressed BMP-receptor-1b; MCHT-1/26; ATDC-5) and via the influence of the extracts on the expression of osteogenic/chondrogenic genes and proteins in human adipose tissue-derived mesenchymal stem cells (hASCs). There was hardly any BMP release in PBS, whereas in medium + FCS or sheep serum the cumulative release over 30/31 days was 11–34% for GDF5 and 6–17% for BB-1; the release of BMP-2 over 14 days was 25.7%. Addition of 10% PLGA fibers significantly augmented the 14-day release of GDF5 and BMP-2 (to 22.6% and 43.7%, respectively), but not of BB-1 (13.2%). All BMPs proved to be bioactive, as demonstrated by increased ALP activity in several cell lines, with partial enhancement by 10% PLGA fibers, and by a specific, early regulation of osteogenic/chondrogenic genes and proteins in hASCs. Between 10% and 45% of bioactive BMPs were released in vitro from CPC + PLGA fibers over a time period of 14 days, providing a basis for estimating and tailoring therapeutically effective doses for experimental and human in vivo studies.

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