Conductive dual hydrogen bonding hydrogels for the electrical stimulation of infected chronic wounds

2021 ◽  
Vol 9 (38) ◽  
pp. 8138-8146
Author(s):  
Ye Wu ◽  
Yuhui Lu ◽  
Can Wu ◽  
Jiali Chen ◽  
Ning Ning ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Antimicrobial Activity ◽  
Hydrogen Bonding ◽  
Electrical Stimulation ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Great Promise ◽  
Wound Treatment ◽  
Conductive Hydrogels ◽  
Stimulation Of

Polyaniline provides skin-mimic electrical conductivity and functionalized quaternary ammonium groups show intense antimicrobial activity. Electrical stimulation using conductive hydrogels shows great promise for chronic wound treatment.

scholarly journals ASCENT (Automated Simulations to Characterize Electrical Nerve Thresholds): A pipeline for sample-specific computational modeling of electrical stimulation of peripheral nerves

2021 ◽  
Vol 17 (9) ◽  
pp. e1009285
Author(s):  
Eric D. Musselman ◽  
Jake E. Cariello ◽  
Warren M. Grill ◽  
Nicole A. Pelot
Keyword(s):  
Electrical Stimulation ◽  
Computational Modeling ◽  
Peripheral Nerves ◽  
Nerve Fibers ◽  
Great Promise ◽  
Simulation Data ◽  
Stimulation Parameters ◽  
Neural Anatomy ◽  
Disease And Disorders ◽  
Stimulation Of

Electrical stimulation and block of peripheral nerves hold great promise for treatment of a range of disease and disorders, but promising results from preclinical studies often fail to translate to successful clinical therapies. Differences in neural anatomy across species require different electrodes and stimulation parameters to achieve equivalent nerve responses, and accounting for the consequences of these factors is difficult. We describe the implementation, validation, and application of a standardized, modular, and scalable computational modeling pipeline for biophysical simulations of electrical activation and block of nerve fibers within peripheral nerves. The ASCENT (Automated Simulations to Characterize Electrical Nerve Thresholds) pipeline provides a suite of built-in capabilities for user control over the entire workflow, including libraries for parts to assemble electrodes, electrical properties of biological materials, previously published fiber models, and common stimulation waveforms. We validated the accuracy of ASCENT calculations, verified usability in beta release, and provide several compelling examples of ASCENT-implemented models. ASCENT will enable the reproducibility of simulation data, and it will be used as a component of integrated simulations with other models (e.g., organ system models), to interpret experimental results, and to design experimental and clinical interventions for the advancement of peripheral nerve stimulation therapies.

scholarly journals Antlerogenic stem cells extract accelerate chronic wound healing: a preliminary study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Janusz Kmiecik ◽  
Michał Jerzy Kulus ◽  
Jarosław Popiel ◽  
Agnieszka Cekiera ◽  
Marek Cegielski
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Wound Care ◽  
Small Population ◽  
Area Ratio ◽  
Wound Treatment ◽  
Ki 67 ◽  
Pressure Therapy

Abstract Background Chronic wounds constitute a significant medical and social problem. Chronic wound treatment may be supported by various techniques, such as negative pressure therapy, phototherapy or stem cells therapy, yet most of those supporting therapies need more evidence to be used for standard wound care. Current study covers the use of sonicated Antlerogenic Stem Cells (ASC) extract on chronic wounds. Methods Study was performed on 20 dermatological patients with venous leg ulcers, divided into two groups – treated with and without ASC extract respectively. The area and circumference of the wounds during the follow-up visits were measured on the wound imprint. Dynamics of wound healing was determined and compared between control and study group; statistics includes changes in absolute values (wound area, circumference), as well as relative (percentage of wound decrease, circumference/area ratio) and their change in time. For the purpose of Ki-67 immunohistochemical staining, sections were sampled from the wound edge at distinct check-points during therapy. Results of both groups were compared with Student test or Mann-Whitney test, depending on results distribution. Results Besides Ki-67 expression, all tested wound healing parameters (including relative and absolute wound decrease and changes in circumference/area ratio) were statistically significant more favorable in experimental group. Conclusion ASC extract significantly supported standard chronic wound treatment. Due to small population of study the results should be considered preliminary, yet promising for further research.

In situ gelation of rhEGF-containing liquid crystalline precursor with good cargo stability and system mechanical properties: a novel delivery system for chronic wounds treatment

2019 ◽  
Vol 7 (3) ◽  
pp. 995-1010 ◽  
Author(s):  
Chan Zhou ◽  
Zhengwei Huang ◽  
Ying Huang ◽  
Bei Wang ◽  
Peipei Yang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Growth Factor ◽  
Delivery System ◽  
Liquid Crystalline ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Wound Treatment ◽  
In Situ Gelation ◽  
Epidermal Growth

The objective of this study was to develop a novel delivery system for recombinant human epidermal growth factor (rhEGF) for chronic wound treatment.

scholarly journals Honey clinically stimulates granulation and epithelialization in chronic wounds: a report of two cases

2018 ◽  
Vol 27 (1) ◽  
pp. 62-8 ◽  
Author(s):  
Putu E.P. Kefani ◽  
Ida B.A.D. Putra ◽  
Ratna R.N. Roosseno
Keyword(s):  
Side Effects ◽  
Sodium Bicarbonate ◽  
Diabetic Foot ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Ulcer Patient ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Wound Treatment

Various studies have been widely reintroduced honey as a wound dressing in chronic wound treatment. However, its utilization in Indonesia is still very limited. This study reported the effect of honey in stimulating the epithelialization and granulation on chronic wound treatment in our center. Two chronic wound cases, diabetic foot ulcer patient and gangrenous extravasation due to sodium bicarbonate, were daily treated with honey applications. After six-week evaluation, clinically the tissue granulation and epithelization were seen at the edge of the observed wounds and decreased the wound size significantly with no reported side effects. Honey clinically stimulates the epithelialization and granulation on chronic wound treatment.

scholarly journals Formulation and characterisation of alginate hydrocolloid film dressing loaded with gallic acid for potential chronic wound healing

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 451
Author(s):  
Jhing-Ee Gan ◽  
Chai-Yee Chin
Keyword(s):  
Gallic Acid ◽  
Sodium Alginate ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Moisture Absorption ◽  
Chronic Wound ◽  
Chemical Interaction ◽  
Transmission Rate ◽  
Bioactive Compound ◽  
Wound Treatment

Background: A dramatic growth in the prevalence of chronic wounds due to diabetes has represented serious global health care and economic issues. Hence, there is an imperative need to develop an effective and affordable wound dressing for chronic wounds. Recent research has featured the potential of bioactive compound gallic acid (GA) in the context of wound recovery due to their safety and comparatively low cost. However, there is a scarcity of research that focuses on formulating GA into a stable and functional hydrocolloid film dressing. Thus, this present study aimed to formulate and characterise GA-loaded alginate-based hydrocolloid film dressing which is potentially used as low to medium suppurating chronic wound treatment. Methods: The hydrocolloid composite films were pre-formulated by blending sodium alginate (SA) with different combinations of polymers. The hydrocolloid films were developed using solvent-casting method and the most satisfactory film formulation was further incorporated with various GA concentrations (0.1%, 0.5% and 1%). The drug-loaded films were then characterised for their physicochemical properties to assess their potential use as drug delivery systems for chronic wound treatment. Results: In the pre-formulation studies, sodium alginate-pectin (SA-PC) based hydrocolloid film was found to be the most satisfactory, for being homogenous and retaining smoothness on surface along with satisfactory film flexibility. The SA-PC film was chosen for further loading with GA in 0.1%, 0.5% and 1%. The characterisation studies revealed that all GA-loaded films possess superior wound dressing properties of acidic pH range (3.97-4.04), moderate viscosity (1600 mPa-s-3198 mPa-s), optimal  moisture vapor transmission rate (1195 g/m2/day, 1237g/m2/day and 1112 g/m2/day), slower moisture absorption and film expansion rate and no chemical interaction between the GA and polymers under FTIR analysis. Conclusion: An SA-PC hydrocolloid film incorporated with gallic acid as a potentially applicable wound dressing for low to medium suppurating chronic wounds was successfully developed.

scholarly journals A Concise Review on Tissue Engineered Artificial Skin Grafts for Chronic Wound Treatment: Can We Reconstruct Functional Skin Tissue In Vitro?

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1622 ◽  
Author(s):  
Agata Przekora
Keyword(s):  
Chronic Wounds ◽  
Chronic Wound ◽  
Healing Process ◽  
Topical Delivery ◽  
Artificial Skin ◽  
Skin Tissue ◽  
Wound Treatment ◽  
Skin Grafts ◽  
Skin Substitutes ◽  
Modern Approach

Chronic wounds occur as a consequence of a prolonged inflammatory phase during the healing process, which precludes skin regeneration. Typical treatment for chronic wounds includes application of autografts, allografts collected from cadaver, and topical delivery of antioxidant, anti-inflammatory, and antibacterial agents. Nevertheless, the mentioned therapies are not sufficient for extensive or deep wounds. Moreover, application of allogeneic skin grafts carries high risk of rejection and treatment failure. Advanced therapies for chronic wounds involve application of bioengineered artificial skin substitutes to overcome graft rejection as well as topical delivery of mesenchymal stem cells to reduce inflammation and accelerate the healing process. This review focuses on the concept of skin tissue engineering, which is a modern approach to chronic wound treatment. The aim of the article is to summarize common therapies for chronic wounds and recent achievements in the development of bioengineered artificial skin constructs, including analysis of biomaterials and cells widely used for skin graft production. This review also presents attempts to reconstruct nerves, pigmentation, and skin appendages (hair follicles, sweat glands) using artificial skin grafts as well as recent trends in the engineering of biomaterials, aiming to produce nanocomposite skin substitutes (nanofilled polymer composites) with controlled antibacterial activity. Finally, the article describes the composition, advantages, and limitations of both newly developed and commercially available bioengineered skin substitutes.

scholarly journals Cellular Response to Vitamin C-Enriched Chitosan/Agarose Film with Potential Application as Artificial Skin Substitute for Chronic Wound Treatment

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1185 ◽  
Author(s):  
Vladyslav Vivcharenko ◽  
Michal Wojcik ◽  
Agata Przekora
Keyword(s):  
Growth Factor ◽  
Vitamin C ◽  
Human Skin ◽  
Cellular Response ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Artificial Skin ◽  
Skin Substitute ◽  
Wound Treatment ◽  
Human Skin Fibroblasts

The treatment of chronic wounds is still a meaningful challenge to physicians. The aim of this work was to produce vitamin C-enriched chitosan/agarose (CHN/A) film that could serve as potential artificial skin substitute for chronic wound treatment. The biomaterial was fabricated by a newly developed and simplified method via mixing acidic chitosan solution with alkaline agarose solution that allowed to obtain slightly acidic pH (5.97) of the resultant material, which is known to support skin regeneration. Vitamin C was immobilized within the matrix of the film by entrapment method during production process. Produced films (CHN/A and CHN/A + vit C) were subjected to comprehensive evaluation of cellular response with the use of human skin fibroblasts, epidermal keratinocytes, and macrophages. It was demonstrated that novel biomaterials support adhesion and growth of human skin fibroblasts and keratinocytes, have ability to slightly reduce transforming growth factor-beta 1 (TGF-β1) (known to be present at augmented levels in the epidermis of chronic wounds), and increase platelet-derived growth factor-BB (PDGF-BB) secretion by the cells. Nevertheless, addition of vitamin C to the biomaterial formulation does not significantly improve its biological properties due to burst vitamin release profile. Obtained results clearly demonstrated that produced CHN/A film has great potential to be used as cellular dermal, epidermal, or dermo-epidermal graft pre-seeded with human skin cells for chronic wound treatment.

scholarly journals Antibiofilm Efficacy of DispersinB® Wound Spray Used in Combination with a Silver Wound Dressing

2014 ◽  
Vol 7 ◽  
pp. MBI.S13914 ◽  
Author(s):  
Purushottam V. Gawande ◽  
Allie P. Clinton ◽  
Karen LoVetri ◽  
Nandadeva Yakandawala ◽  
Kendra P. Rumbaugh ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Wound Management ◽  
Potential Applications ◽  
Mrsa Infection ◽  
Chronic Wound Management ◽  
Methods Of Treatment

Chronic wounds including diabetic foot ulcers, pressure ulcers, and venous leg ulcers are a worldwide health problem. As the traditional methods of treatment have proven ineffective against chronic wounds involving biofilms, there is an unmet clinical need for developing products with an antibiofilm component that inhibits and/or disrupts biofilms and thus make the biofilm-embedded bacteria more susceptible to antimicrobial therapy. We developed a DispersinB® antibiofilm enzyme-based wound spray for treating chronic wounds in conjunction with an antimicrobial. Under in vitro conditions, the DispersinB® and Acticoat™ combination performed significantly better ( P < 0.05) than Acticoat™ alone, indicating the synergy between the two compounds because of DispersinB® enhancing the antimicrobial activity of Acticoat™. Furthermore, DispersinB® wound spray enhanced the antimicrobial activity of Acticoat™ in a chronic wound mouse model of methicillin-resistant Staphylococcus aureus (MRSA) infection. Thus, this novel combination of DispersinB® and Acticoat™, an antimicrobial dressing, prompts clinical evaluation for potential applications in biofilm-based chronic wound management.

scholarly journals Electrical Stimulation to Enhance Wound Healing

2021 ◽  
Vol 12 (2) ◽  
pp. 40
Author(s):  
Saranya B. Rajendran ◽  
Kirsty Challen ◽  
Karen L. Wright ◽  
John G. Hardy
Keyword(s):  
Wound Healing ◽  
Electrical Stimulation ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Financial Burden ◽  
Cochrane Library ◽  
Publication Date ◽  
Pulsed Current ◽  
Fibroblast Migration

Electrical stimulation (ES) can serve as a therapeutic modality accelerating the healing of wounds, particularly chronic wounds which have impaired healing due to complications from underlying pathology. This review explores how ES affects the cellular mechanisms of wound healing, and its effectiveness in treating acute and chronic wounds. Literature searches with no publication date restrictions were conducted using the Cochrane Library, Medline, Web of Science, Google Scholar and PubMed databases, and 30 full-text articles met the inclusion criteria. In vitro and in vivo experiments investigating the effect of ES on the general mechanisms of healing demonstrated increased epithelialization, fibroblast migration, and vascularity around wounds. Six in vitro studies demonstrated bactericidal effects upon exposure to alternating and pulsed current. Twelve randomized controlled trials (RCTs) investigated the effect of pulsed current on chronic wound healing. All reviewed RCTs demonstrated a larger reduction in wound size and increased healing rate when compared to control groups. In conclusion, ES therapy can contribute to improved chronic wound healing and potentially reduce the financial burden associated with wound management. However, the variations in the wound characteristics, patient demographics, and ES parameters used across studies present opportunities for systematic RCT studies in the future.

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