scholarly journals Cellular Response to Vitamin C-Enriched Chitosan/Agarose Film with Potential Application as Artificial Skin Substitute for Chronic Wound Treatment

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1185 ◽  
Author(s):  
Vladyslav Vivcharenko ◽  
Michal Wojcik ◽  
Agata Przekora
Keyword(s):  
Growth Factor ◽  
Vitamin C ◽  
Human Skin ◽  
Cellular Response ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Artificial Skin ◽  
Skin Substitute ◽  
Wound Treatment ◽  
Human Skin Fibroblasts

The treatment of chronic wounds is still a meaningful challenge to physicians. The aim of this work was to produce vitamin C-enriched chitosan/agarose (CHN/A) film that could serve as potential artificial skin substitute for chronic wound treatment. The biomaterial was fabricated by a newly developed and simplified method via mixing acidic chitosan solution with alkaline agarose solution that allowed to obtain slightly acidic pH (5.97) of the resultant material, which is known to support skin regeneration. Vitamin C was immobilized within the matrix of the film by entrapment method during production process. Produced films (CHN/A and CHN/A + vit C) were subjected to comprehensive evaluation of cellular response with the use of human skin fibroblasts, epidermal keratinocytes, and macrophages. It was demonstrated that novel biomaterials support adhesion and growth of human skin fibroblasts and keratinocytes, have ability to slightly reduce transforming growth factor-beta 1 (TGF-β1) (known to be present at augmented levels in the epidermis of chronic wounds), and increase platelet-derived growth factor-BB (PDGF-BB) secretion by the cells. Nevertheless, addition of vitamin C to the biomaterial formulation does not significantly improve its biological properties due to burst vitamin release profile. Obtained results clearly demonstrated that produced CHN/A film has great potential to be used as cellular dermal, epidermal, or dermo-epidermal graft pre-seeded with human skin cells for chronic wound treatment.

In situ gelation of rhEGF-containing liquid crystalline precursor with good cargo stability and system mechanical properties: a novel delivery system for chronic wounds treatment

2019 ◽  
Vol 7 (3) ◽  
pp. 995-1010 ◽  
Author(s):  
Chan Zhou ◽  
Zhengwei Huang ◽  
Ying Huang ◽  
Bei Wang ◽  
Peipei Yang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Growth Factor ◽  
Delivery System ◽  
Liquid Crystalline ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Wound Treatment ◽  
In Situ Gelation ◽  
Epidermal Growth

The objective of this study was to develop a novel delivery system for recombinant human epidermal growth factor (rhEGF) for chronic wound treatment.

scholarly journals A Concise Review on Tissue Engineered Artificial Skin Grafts for Chronic Wound Treatment: Can We Reconstruct Functional Skin Tissue In Vitro?

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1622 ◽  
Author(s):  
Agata Przekora
Keyword(s):  
Chronic Wounds ◽  
Chronic Wound ◽  
Healing Process ◽  
Topical Delivery ◽  
Artificial Skin ◽  
Skin Tissue ◽  
Wound Treatment ◽  
Skin Grafts ◽  
Skin Substitutes ◽  
Modern Approach

Chronic wounds occur as a consequence of a prolonged inflammatory phase during the healing process, which precludes skin regeneration. Typical treatment for chronic wounds includes application of autografts, allografts collected from cadaver, and topical delivery of antioxidant, anti-inflammatory, and antibacterial agents. Nevertheless, the mentioned therapies are not sufficient for extensive or deep wounds. Moreover, application of allogeneic skin grafts carries high risk of rejection and treatment failure. Advanced therapies for chronic wounds involve application of bioengineered artificial skin substitutes to overcome graft rejection as well as topical delivery of mesenchymal stem cells to reduce inflammation and accelerate the healing process. This review focuses on the concept of skin tissue engineering, which is a modern approach to chronic wound treatment. The aim of the article is to summarize common therapies for chronic wounds and recent achievements in the development of bioengineered artificial skin constructs, including analysis of biomaterials and cells widely used for skin graft production. This review also presents attempts to reconstruct nerves, pigmentation, and skin appendages (hair follicles, sweat glands) using artificial skin grafts as well as recent trends in the engineering of biomaterials, aiming to produce nanocomposite skin substitutes (nanofilled polymer composites) with controlled antibacterial activity. Finally, the article describes the composition, advantages, and limitations of both newly developed and commercially available bioengineered skin substitutes.

SYNERGISM BETWEEN INTERFERON-γ AND cAMP IN INDUCTION OF HEPATOCYTE GROWTH FACTOR IN HUMAN SKIN FIBROBLASTS

Cytokine ◽  
2000 ◽  
Vol 12 (6) ◽  
pp. 780-785 ◽  
Author(s):  
Eiichi Gohda ◽  
Kazunori Kuromitsu ◽  
Tetsuhiko Matsunaga ◽  
Masahiro Miyazaki ◽  
Itaru Yamamoto
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Human Skin ◽  
Interferon Γ ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Hepatocyte Growth
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