Se-modified gold nanorods for enhancing the photothermal therapy efficiency--avoiding the off-target problem induced by biothiols

2021 ◽  
Author(s):  
Bo Hu ◽  
Zengteng Zhao ◽  
Xiaonan Gao ◽  
Xiaoxiao Song ◽  
Zihao Xu ◽  
...  
Keyword(s):  
Intravenous Injection ◽  
Gold Nanorods ◽  
Photothermal Therapy ◽  
Target Problem ◽  
Long Time

Tumor-targeted gold nanorods (AuNRs) assembled through Au-S bond have been widely used for photothermal therapy (PTT) via intravenous injection. However, with long time in vivo circulation, biothiols can replace some...

scholarly journals Efficacy, long-term toxicity, and mechanistic studies of gold nanorods photothermal therapy of cancer in xenograft mice

2017 ◽  
Vol 114 (15) ◽  
pp. E3110-E3118 ◽  
Author(s):  
Moustafa R. K. Ali ◽  
Mohammad Aminur Rahman ◽  
Yue Wu ◽  
Tiegang Han ◽  
Xianghong Peng ◽  
...  
Keyword(s):  
Cell Death ◽  
Mechanism Of Action ◽  
Gold Nanorods ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Infrared Light ◽  
Mouse Tumor ◽  
Maximal Induction

Gold nanorods (AuNRs)-assisted plasmonic photothermal therapy (AuNRs-PPTT) is a promising strategy for combating cancer in which AuNRs absorb near-infrared light and convert it into heat, causing cell death mainly by apoptosis and/or necrosis. Developing a valid PPTT that induces cancer cell apoptosis and avoids necrosis in vivo and exploring its molecular mechanism of action is of great importance. Furthermore, assessment of the long-term fate of the AuNRs after treatment is critical for clinical use. We first optimized the size, surface modification [rifampicin (RF) conjugation], and concentration (2.5 nM) of AuNRs and the PPTT laser power (2 W/cm2) to achieve maximal induction of apoptosis. Second, we studied the potential mechanism of action of AuNRs-PPTT using quantitative proteomic analysis in mouse tumor tissues. Several death pathways were identified, mainly involving apoptosis and cell death by releasing neutrophil extracellular traps (NETs) (NETosis), which were more obvious upon PPTT using RF-conjugated AuNRs (AuNRs@RF) than with polyethylene glycol thiol-conjugated AuNRs. Cytochrome c and p53-related apoptosis mechanisms were identified as contributing to the enhanced effect of PPTT with AuNRs@RF. Furthermore, Pin1 and IL18-related signaling contributed to the observed perturbation of the NETosis pathway by PPTT with AuNRs@RF. Third, we report a 15-month toxicity study that showed no long-term toxicity of AuNRs in vivo. Together, these data demonstrate that our AuNRs-PPTT platform is effective and safe for cancer therapy in mouse models. These findings provide a strong framework for the translation of PPTT to the clinic.

Ultrasound and photoacoustic image-guided photothermal therapy using silica-coated gold nanorods: In-vivo study

2010 ◽  
Author(s):  
Seungsoo Kim ◽  
Yun-Sheng Chen ◽  
Geoffrey P. Luke ◽  
Mohammad Mehrmohammadi ◽  
Jason R. Cook ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Photothermal Therapy ◽  
In Vivo Study ◽  
Image Guided

scholarly journals DARPin_9-29-Targeted Gold Nanorods Selectively Suppress HER2-Positive Tumor Growth in Mice

Cancers ◽  
2021 ◽  
Vol 13 (20) ◽  
pp. 5235
Author(s):  
Galina M. Proshkina ◽  
Elena I. Shramova ◽  
Marya V. Shilova ◽  
Ivan V. Zelepukin ◽  
Victoria O. Shipunova ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Targeted Delivery ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Therapeutic Potential ◽  
Infrared Light ◽  
Strong Reduction ◽  
Her2 Positive ◽  
Excellent Stability

Near-infrared phototherapy has great therapeutic potential for cancer treatment. However, for efficient application, in vivo photothermal agents should demonstrate excellent stability in blood and targeted delivery to pathological tissue. Here, we demonstrated that stable bovine serum albumin-coated gold mini nanorods conjugated to a HER2-specific designed ankyrin repeat protein, DARPin_9-29, selectively accumulate in HER2-positive xenograft tumors in mice and lead to a strong reduction in the tumor size when being illuminated with near-infrared light. The results pave the way for the development of novel DARPin-based targeted photothermal therapy of cancer.

scholarly journals Theranostic Approach for Cancer Treatment: Multifunctional Gold Nanorods for Optical Imaging and Photothermal Therapy

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Oshra Betzer ◽  
Rinat Ankri ◽  
Menachem Motiei ◽  
Rachela Popovtzer
Keyword(s):  
Gold Nanorods ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Detection Method ◽  
Nir Spectroscopy ◽  
Surgical Excision ◽  
Spectral Shift ◽  
Tissue Destruction ◽  
Shift Analysis

A critical problem in the treatment of cancer is the inability to identify microsized tumors and treat them without normal tissue destruction. While surgical excision of tumors is highly effective, residual micrometastases and remaining positive margins are the main cause of recurrence. In this study, we propose a theranostic approach for the detection and therapy of head and neck cancer (HNC). We developed a plasmonic-based nanoplatform for combined, ultrasensitivein vivospectroscopic detection and targeted therapy of HNC. This detection method involves near-infrared (NIR) spectroscopy of gold nanorods (GNRs) that selectively target and attach to squamous cell carcinoma HNC cells, through an immune complex. Diagnosis is based on a spectral shift analysis, which is generated by interparticle-plasmon-resonance patterns of the specifically targeted GNRs. Additionally, the ability to design the GNRs to strongly absorb light in the NIR region enables efficient irradiation of these GNRs, for selective photothermal therapy (PTT) of the cancer cells. We expect this targeted, noninvasive, and nonionizing spectroscopic detection method to provide a highly sensitive and simple diagnostic tool for micrometastasis. In addition, the concomitant development of targeted PTT, based on specific cancer markers, may pave the way for tailoring effective therapy for patients, toward an era of personalized medicine.

THE IN VIVO INVESTIGATION OF Fe3O4-NANOPARTICLES ACUTE TOXICITY IN MICE

2012 ◽  
Vol 24 (03) ◽  
pp. 229-235 ◽  
Author(s):  
Shuhua Zhao ◽  
Xinli Lin ◽  
Long Zhang ◽  
Lei Sun ◽  
Jun Li ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Confidence Interval ◽  
Intravenous Injection ◽  
Cardiac Tissue ◽  
Kidney Tubules ◽  
Tissue Protein ◽  
Long Time ◽  
Different Doses

To observe acute toxicity of naked Fe3O4 -nanoparticles in mice, ICR mice were selected and exposed once to naked Fe3O4 -nanoparticles by tail intravenous injection with different doses, i.e. 0, 102.4, 128, 160, 200, 250 mg/kg of body weight. 15 days later, Fe3O4 distribution and pathologic changes in main organs were investigated. By tail intravenous injection, LD50 of naked Fe3O4 -nanoparticles in mice was 163.60 mg/kg of body weight (147.58, 181.37 mg/kg of body weight, 95% confidence interval). Deaths of mice mainly caused by decompensation and twitching were observed. Immediately after injection, nanoparticles performed fast distribution in lung, liver, spleen etc, yet little were traced in brain, heart and kidney. 15 days after injection, apparent decline of nanoparticles content in lung and spleen was observed, whereas in liver the content rose. Pathologic detection indicated local particle denaturation and necrosis in the cardiac tissue. Protein cast was seen in several kidney tubules, and extravasated blood in carunculae papillaris, yet no pathologic changes were observed in other organs. LD50 of Fe3O4 nanoparticles (9 nm) by means of tail intravenous injection is 163.60 mg/kg, and they mainly distributed in liver, spleen, lung and caused denaturation and necrosis in the cardiac muscle and malfunction of kidney. Also, the process of excreting the particles takes a long time.

Gold nanorods and curcumin-loaded nanomicelles for efficient in vivo photothermal therapy of Barrett's esophagus

Nanomedicine ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. 1723-1733 ◽  
Author(s):  
Robert CG Martin ◽  
Erica Locatelli ◽  
Yan Li ◽  
Weizhong Zhang ◽  
Suping Li ◽  
...  
Keyword(s):  
Barrett’S Esophagus ◽  
Gold Nanorods ◽  
Barrett's Esophagus ◽  
Photothermal Therapy

Gold nanorods as a theranostic platform for in vitro and in vivo imaging and photothermal therapy of inflammatory macrophages

Nanoscale ◽  
2015 ◽  
Vol 7 (33) ◽  
pp. 13991-14001 ◽  
Author(s):  
Jinbao Qin ◽  
Zhiyou Peng ◽  
Bo Li ◽  
Kaichuang Ye ◽  
Yuxin Zhang ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
In Vivo Imaging ◽  
Photothermal Therapy ◽  
Au Nanorods ◽  
Associated Diseases ◽  
Inflammatory Macrophages

This study shows that Au nanorods are a promising theranostic platform for the diagnosis and photothermal therapy of inflammation associated diseases.

RGD-Conjugated Dendrimer-Modified Gold Nanorods for in Vivo Tumor Targeting and Photothermal Therapy

2009 ◽  
Vol 7 (1) ◽  
pp. 94-104 ◽  
Author(s):  
Zhiming Li ◽  
Peng Huang ◽  
Xuejun Zhang ◽  
Jing Lin ◽  
Sen Yang ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Photothermal Therapy ◽  
Tumor Targeting
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