Amine-functionalized Cu-MOF Nanospheres towards Label-free Hepatitis B Surface Antigen Electrochemical Immunosensors

2021 ◽  
Author(s):  
Muhammad Rezki ◽  
Ni Luh Wulan Septiani ◽  
Muhammad Iqbal ◽  
Suksmandhira Harimurti ◽  
Poetro Sambegoro ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Metal Organic Framework ◽  
Label Free ◽  
Facile Synthesis ◽  
Amine Functionalized ◽  
Electrochemical Immunosensors ◽  
Wide Range ◽  
Metal Organic

Metal-organic framework (MOF) nanomaterials offer a wide range of promising applications due to their unique properties, including open micro- and mesopores and rich of functionalization. Herein, a facile synthesis via...

scholarly journals Measurement of Hepatitis B Surface Antigen Concentrations Using a Piezoelectric Microcantilever as a Mass Sensor

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Sangkyu Lee ◽  
Jongyun Cho ◽  
Yeolho Lee ◽  
Sangmin Jeon ◽  
Hyung Joon Cha ◽  
...  
Keyword(s):  
Reaction Time ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Environmental Conditions ◽  
Surface Stress ◽  
Hepatitis B Surface Antigen ◽  
Spring Constant ◽  
Label Free ◽  
Mass Sensor ◽  
Piezoelectric Microcantilever

Hepatitis B surface antigen (HBsAg) concentrations were measured using a piezoelectric microcantilever sensor (PEMS) developed by the authors. The developed PEMS is label-free and detects the sensing signal electrically. It was designed to measure the mass of biomolecules attached to it using an accurate mass-microbalancing technique; its probe area is confined to the end of the cantilever, and its equivalent spring constant is relatively high to minimize the effect of changes in the surface stress when the biomolecules are attached to it. The “dip- and-dry” technique was used to enable the probe area of the sensor to react with reagents in controlled environmental conditions. HBsAg was detected by an immunoreaction whereas the reaction time, antibody density, and its area on the probe were kept at a constant level. The mass of the detected HBsAg was measured in the range of 0.1–100 ng/mL.

Au@Zr-based metal–organic framework composite as an immunosensing platform for determination of hepatitis B virus surface antigen

2021 ◽  
Vol 188 (11) ◽  
Author(s):  
Vivek K. Bajpai ◽  
Yuvraj Haldorai ◽  
Imran Khan ◽  
Sonam Sonwal ◽  
Mahendra Pal Singh ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Metal Organic Framework ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus ◽  
Metal Organic ◽  
Organic Framework

scholarly journals Multicenter Evaluation of the Elecsys Hepatitis B Surface Antigen Quantitative Assay

2011 ◽  
Vol 18 (11) ◽  
pp. 1943-1950 ◽  
Author(s):  
B. J. Zacher ◽  
F. Moriconi ◽  
S. Bowden ◽  
R. Hammond ◽  
S. Louisirirotchanakul ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
World Health ◽  
Quantitative Assay ◽  
Clinical Samples ◽  
High Dose ◽  
Serum Samples ◽  
Hook Effect ◽  
Wide Range

ABSTRACTThe Elecsys hepatitis B surface antigen (HBsAg) II quantitative assay is a new quantitative electrochemiluminescence immunoassay which uses onboard dilution and a simple algorithm to determine HBsAg levels expressed in international units (IU)/ml (standardized against the World Health Organization [WHO] Second International Standard). This study evaluated its performance using routine serum samples from a wide range of HBsAg carriers and patients with chronic hepatitis B (CHB). HBsAg levels were measured in serum samples collected independently by five centers in Europe, Australia, and Asia. Serial dilution analyses were performed to assess the recommended dilution algorithm and determine the assay range free of hook effect. Assay precision was also established. Following assessment of serial dilutions (1:100 to 1:1,000,000) of the 611 samples analyzed, 70.0% and 85.6% of samples tested with analyzers incorporating 1:100 (Elecsys 2010 and cobas e 411) and 1:400 (Modular Analytics E170) onboard dilution, respectively, fell within the linear range of the assay, providing a final result on the first test. No high-dose hook effect was seen up to the maximum HBsAg serum level tested (870,000 IU/ml) using the dilution algorithm. HBsAg levels were reliably determined across all hepatitis B virus (HBV) genotypes, phases of HBV infection, and stages of disease tested. Precision was high across all analyzers (% coefficient of variation [CV], 1.4 to 9.6; HBsAg concentrations, 0.1 to 37,300 IU/ml). The Elecsys HBsAg II quantitative assay accurately and reliably quantifies HBsAg in routine clinical samples. Onboard dilution minimizes retesting and reduces the potential for error.

Facile synthesis of MoS2@Cu2O-Pt nanohybrid as enzyme-mimetic label for the detection of the Hepatitis B surface antigen

2018 ◽  
Vol 100 ◽  
pp. 512-518 ◽  
Author(s):  
Faying Li ◽  
Yueyun Li ◽  
Jinhui Feng ◽  
Zengqiang Gao ◽  
Hui Lv ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Facile Synthesis ◽  
Enzyme Mimetic

A label-free immunosensor based on PtPd NCs@MoS2 nanoenzymes for hepatitis B surface antigen detection

2019 ◽  
Vol 142 ◽  
pp. 111556 ◽  
Author(s):  
Zhaoling Tan ◽  
Hui Dong ◽  
Qing Liu ◽  
Hui Liu ◽  
Pingping Zhao ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Antigen Detection ◽  
Label Free

A novel label-free electrochemical immunosensor for the detection of hepatitis B surface antigen

2016 ◽  
Vol 8 (40) ◽  
pp. 7380-7386 ◽  
Author(s):  
Lihui Tian ◽  
Li Liu ◽  
Yueyuan Li ◽  
Xue Feng ◽  
Qin Wei ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B ◽  
Reduced Graphene Oxide ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Electrochemical Immunosensor ◽  
Quantitative Detection ◽  
Label Free ◽  
Graphene Oxide Nanosheets ◽  
Reduced Graphene

A novel label-free electrochemical immunosensor based on trimetallic NiAuPt nanoparticles on reduced graphene oxide nanosheets (NiAuPt–NGs) was developed for the quantitative detection of hepatitis B surface antigen (HBsAg).

IL10 Promotorpolymorphismen beeinflussen die Hepatitis B Surface Antigen und die Hepatitis A spezifische Immunantwort

2015 ◽  
Vol 41 (08) ◽  
Author(s):  
E Reuss ◽  
N Evers ◽  
N Dietrich ◽  
J Vollmar ◽  
PM Schneider ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis A ◽  
Hepatitis B Surface Antigen
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