A novel label-free electrochemical immunosensor for the detection of hepatitis B surface antigen

2016 ◽  
Vol 8 (40) ◽  
pp. 7380-7386 ◽  
Author(s):  
Lihui Tian ◽  
Li Liu ◽  
Yueyuan Li ◽  
Xue Feng ◽  
Qin Wei ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B ◽  
Reduced Graphene Oxide ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Electrochemical Immunosensor ◽  
Quantitative Detection ◽  
Label Free ◽  
Graphene Oxide Nanosheets ◽  
Reduced Graphene

A novel label-free electrochemical immunosensor based on trimetallic NiAuPt nanoparticles on reduced graphene oxide nanosheets (NiAuPt–NGs) was developed for the quantitative detection of hepatitis B surface antigen (HBsAg).

Label-free electrochemical immunosensor based on gold nanoparticle/polyethyleneimine/reduced graphene oxide nanocomposite for the ultrasensitive detection of cancer biomarker matrix metalloproteinase-1

The Analyst ◽  
2021 ◽  
Author(s):  
Xinke Liu ◽  
Lu-Yin Lin ◽  
Fu-Yen Tseng ◽  
Yu-Cheng Tan ◽  
Jian Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Matrix Metalloproteinase ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Matrix Metalloproteinase 1 ◽  
Reduced Graphene ◽  
P Matrix

Matrix metalloproteinase-1 (MMP-1) is associated with many types of cancers, including oral, colorectal, and brain cancers. This paper describes the fabrication of an MMP-1 immunosensor based on a gold nanoparticle/polyethyleneimine/reduced...

An Electrochemical Immunosensor with Graphene-Oxide-Ferrocene-based Nanocomposites for Hepatitis B Surface Antigen Detection

2018 ◽  
Vol 30 (11) ◽  
pp. 2774-2780 ◽  
Author(s):  
Feijun Zhao ◽  
Yan Bai ◽  
Ruosheng Zeng ◽  
Liangli Cao ◽  
Jianming Zhu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Electrochemical Immunosensor ◽  
Antigen Detection

Nonenzymatic Electrochemical Immunosensor Using Ferroferric Oxide–Manganese Dioxide–Reduced Graphene Oxide Nanocomposite as Label for α-Fetoprotein Detection

NANO ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. 1650116 ◽  
Author(s):  
Wensi Jian ◽  
Chunping Wang ◽  
Zuanguang Chen ◽  
Yanyan Yu ◽  
Duanping Sun ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Manganese Dioxide ◽  
Reduced Graphene Oxide ◽  
Photoelectron Spectroscopy ◽  
Low Cost ◽  
Synergetic Effect ◽  
Electrochemical Immunosensor ◽  
Quantitative Detection ◽  
Ferroferric Oxide ◽  
Reduced Graphene

A novel nonenzymatic electrochemical immunosensor was fabricated for quantitative detection of [Formula: see text]-fetoprotein (AFP). The immunosensor was constructed by modifying gold electrode with electrochemical reduction of graphene oxide-carboxyl multi-walled carbon nanotube composites (ERGO–CMWCNTs) and electrodeposition of gold nanoparticles (AuNPs) for effective immobilization of primary antibody (Ab[Formula: see text]. Ferroferric oxide–manganese dioxide–reduced graphene oxide nanocomposites (Fe3O4@MnO2–rGO) were designed as labels for signal amplification. On one hand, the excellent electroconductivity and outstanding electron transfer capability of ERGO–CMWCNTs/AuNPs improved the sensitivity of the immunosensor. On the other hand, introduction of rGO could not only increase the specific surface area for immobilization of secondary antibody (Ab[Formula: see text] but also build a synergetic effect to reinforce the electrocatalytic properties of catalysts. Fe3O4@MnO2–rGO nanocomposites were characterized by scanning electron microscope, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Using AFP as a model analyte, the proposed sandwich-type electrochemical immunosensor exhibited a wide linear range of 0.01–50[Formula: see text][Formula: see text] with a low detection limit of 5.8[Formula: see text][Formula: see text]. Moreover, the Fe3O4@MnO2–rGO-based peroxidase mimetic system displayed an excellent analytical performance with low cost, satisfactory reproducibility and high selectivity, which could be further extended for detecting other disease-related biomarkers.

IMMOBILIZATION OF HEPATITIS B ANTIBODY ONTO THE REDUCED GRAPHENE OXIDE

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
G. S. C. Chan ◽  
W. X. F. Wong ◽  
F. C. Chong
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reduced Graphene Oxide ◽  
Surface Antigen ◽  
Virus Surface ◽  
Reduced Graphene ◽  
B Virus ◽  
Secondary Antibodies ◽  
Hep B

Hepatitis B (Hep B) is a viral infection caused by hepatitis B virus (HBV) that infects the human liver. HBV is blood-borne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide. Approximately 5% of the Malaysian population is infected by HBV. The treatment to this disease is possible if early detection is done before irreversible liver damage occurs. The presence of hepatitis B virus surface antigen (HBsAg) is an evidence of the presence of HBV infections. Presently, the conventional methods used for detection show weaknesses in terms of time and efficiency. In this research, the functionality of reduced graphene oxide (rGO) as an immuno biosensor for the detection of HBsAg has been studied. The graphene oxide was prepared via Modified Hummer’s Method due to its ability to produce graphene in large quantity. The fabrication of immunosensor was conducted by immobilizing antibody onto the graphene. nafion-graphene nanosheet was grown and later treated with thionine solution. Following that, a solution of primary antibodies against Hep B surface antigen (anti-HBsAg IgG) was incubated on the biosensor. After the immobilization, the antigen was dropped onto the biosensor, followed by incubation of secondary antibodies conjugated to Horseradish Peroxidase (HRP). Upon addition of Tetramethylbenzidine (TMB), the colour of the solution changes to blue. Thus, proving the functionality of rGO as an immunobiosensor for the detection of HBsAg.

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