scholarly journals A High-Throughput, Solvent Free Method for Dispersing Metal Atoms Directly onto Supports

2021 ◽  
Author(s):  
Emerson Cristofer Kohlrausch ◽  
Higor A. Centurion ◽  
Rhys Lodge ◽  
Xuanli Luo ◽  
Thomas Slater ◽  
...  
Keyword(s):  
High Activity ◽  
High Throughput ◽  
Energy Efficient ◽  
Metal Catalysts ◽  
Solvent Free ◽  
Catalytic Reactions ◽  
Support Materials ◽  
Metal Atoms ◽  
Atom Energy

Atomically-dispersed metal catalysts (ADMCs) on surfaces have demonstrated high activity and selectivity in many catalytic reactions. However, dispersing and stabilising individual atoms in support materials in an atom/energy-efficient scalable way...

Porous and amorphous cobalt hydroxysulfide core–shell nanoneedles on Ti-mesh as a bifunctional electrocatalyst for energy-efficient hydrogen production via urea electrolysis

2021 ◽  
Author(s):  
Yu Jiang ◽  
Shanshan Gao ◽  
Gongchen Xu ◽  
Xiaoming Song
Keyword(s):  
Hydrogen Production ◽  
High Activity ◽  
Energy Efficient ◽  
Bifunctional Catalyst ◽  
Core Shell ◽  
Bifunctional Electrocatalyst ◽  
Urea Electrolysis ◽  
Ti Mesh

Porous and amorphous CoSx(OH)y core–shell nanoneedles covered by numerous ultra-thin small nanosheets are synthesized successfully on Ti-mesh, and act as a high activity and stability bifunctional catalyst for urea electrolysis.

Cooperative Catalytic Reactions Using Organocatalysts and Transition Metal Catalysts: Enantioselective Propargylic Alkylation of Propargylic Esters with Aldehydes

2011 ◽  
Vol 13 (4) ◽  
pp. 592-595 ◽  
Author(s):  
Akiko Yoshida ◽  
Masahiro Ikeda ◽  
Gaku Hattori ◽  
Yoshihiro Miyake ◽  
Yoshiaki Nishibayashi
Keyword(s):  
Transition Metal ◽  
Metal Catalysts ◽  
Transition Metal Catalysts ◽  
Catalytic Reactions ◽  
Propargylic Esters

scholarly journals Coevolution of both Thermostability and Activity of Polyphosphate Glucokinase from Thermobifida fusca YX

2018 ◽  
Vol 84 (16) ◽  
Author(s):  
Wei Zhou ◽  
Rui Huang ◽  
Zhiguang Zhu ◽  
Yi-Heng P. Job Zhang
Keyword(s):  
High Activity ◽  
Double Layer ◽  
High Throughput ◽  
High Throughput Screening ◽  
Specific Activity ◽  
Colorimetric Assay ◽  
Petri Dish ◽  
Rapid Identification ◽  
Thermobifida Fusca ◽  
Content Type

ABSTRACT Thermostability and specific activity of enzymes are two of the most important properties for industrial biocatalysts. Here, we developed a petri dish-based double-layer high-throughput screening (HTS) strategy for rapid identification of desired mutants of polyphosphate glucokinase (PPGK) from a thermophilic actinobacterium, Thermobifida fusca YX, with both enhanced thermostability and activity. Escherichia coli colonies representing a PPGK mutant library were grown on the first-layer Phytagel-based plates, which can remain solid for 1 h, even at heat treatment temperatures of more than 100°C. The second layer that was poured on the first layer contained agarose, substrates, glucose 6-phosphate dehydrogenase (G6PDH), the redox dye tetranitroblue tetrazolium (TNBT), and phenazine methosulfate. G6PDH was able to oxidize the product from the PPGK-catalyzed reaction and generate NADH, which can be easily examined by a TNBT-based colorimetric assay. The best mutant obtained after four rounds of directed evolution had a 7,200-fold longer half-life at 55°C, 19.8°C higher midpoint of unfolding temperature (Tm), and a nearly 3-fold enhancement in specific activities compared to those of the wild-type PPGK. The best mutant was used to produce 9.98 g/liter myo-inositol from 10 g/liter glucose, with a theoretical yield of 99.8%, along with two other hyperthermophilic enzymes at 70°C. This PPGK mutant featuring both great thermostability and high activity would be useful for ATP-free production of glucose 6-phosphate or its derived products.IMPORTANCE Polyphosphate glucokinase (PPGK) is an enzyme that transfers a terminal phosphate group from polyphosphate to glucose, producing glucose 6-phosphate. A petri dish-based double-layer high-throughput screening strategy was developed by using ultrathermostable Phytagel as the first layer instead of agar or agarose, followed by a redox dye-based assay for rapid identification of ultrathermostable PPGK mutants. The best mutant featuring both great thermostability and high activity could produce glucose 6-phosphate from glucose and polyphosphate without in vitro ATP regeneration.

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