scholarly journals Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging

2021 ◽  
Author(s):  
Gaowa Naren ◽  
Wera Larsson ◽  
Carlos Benitez-Martin ◽  
Shiming Li ◽  
Ezequiel Pérez-Inestrosa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Amplitude Modulation ◽  
Water Soluble ◽  
Isomeric Form ◽  
Enhanced Fluorescence ◽  
Bright Fluorescence ◽  
Contrast Enhanced

A water soluble diarylethene derivative displaying exceptionally bright fluorescence in the open isomeric form has been used for emission amplitude-modulation. We apply this scheme in fluorescence microscopy, aiming to suppress undesired background.

scholarly journals Evaluation of bone perfusion during open orthopedic surgery using quantitative dynamic contrast-enhanced fluorescence imaging

2020 ◽  
Vol 11 (11) ◽  
pp. 6458
Author(s):  
I. Leah Gitajn ◽  
Jonathan T. Elliott ◽  
Jason R. Gunn ◽  
Alberto J. Ruiz ◽  
Eric R. Henderson ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Orthopedic Surgery ◽  
Enhanced Fluorescence ◽  
Dynamic Contrast Enhanced ◽  
Contrast Enhanced

Detection of Rheumatoid Arthritis Using Non-Specific Contrast Enhanced Fluorescence Imaging

2010 ◽  
Vol 17 (3) ◽  
pp. 375-381 ◽  
Author(s):  
Thomas Fischer ◽  
Bernd Ebert ◽  
Jan Voigt ◽  
Rainer Macdonald ◽  
Udo Schneider ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Fluorescence Imaging ◽  
Enhanced Fluorescence ◽  
Contrast Enhanced

scholarly journals Low-Light Photodetectors for Fluorescence Microscopy

2021 ◽  
Vol 11 (6) ◽  
pp. 2773
Author(s):  
Hiroaki Yokota ◽  
Atsuhito Fukasawa ◽  
Minako Hirano ◽  
Toru Ide
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Single Molecule ◽  
Science Studies ◽  
Single Photon ◽  
Laser Scanning Microscopy ◽  
Oxide Semiconductor ◽  
Wide Field ◽  
Single Molecule Fluorescence ◽  
Low Light

Over the years, fluorescence microscopy has evolved and has become a necessary element of life science studies. Microscopy has elucidated biological processes in live cells and organisms, and also enabled tracking of biomolecules in real time. Development of highly sensitive photodetectors and light sources, in addition to the evolution of various illumination methods and fluorophores, has helped microscopy acquire single-molecule fluorescence sensitivity, enabling single-molecule fluorescence imaging and detection. Low-light photodetectors used in microscopy are classified into two categories: point photodetectors and wide-field photodetectors. Although point photodetectors, notably photomultiplier tubes (PMTs), have been commonly used in laser scanning microscopy (LSM) with a confocal illumination setup, wide-field photodetectors, such as electron-multiplying charge-coupled devices (EMCCDs) and scientific complementary metal-oxide-semiconductor (sCMOS) cameras have been used in fluorescence imaging. This review focuses on the former low-light point photodetectors and presents their fluorescence microscopy applications and recent progress. These photodetectors include conventional PMTs, single photon avalanche diodes (SPADs), hybrid photodetectors (HPDs), in addition to newly emerging photodetectors, such as silicon photomultipliers (SiPMs) (also known as multi-pixel photon counters (MPPCs)) and superconducting nanowire single photon detectors (SSPDs). In particular, this review shows distinctive features of HPD and application of HPD to wide-field single-molecule fluorescence detection.

scholarly journals Monitoring individual cell-signaling activity using combined metal-clad waveguide and surface-enhanced fluorescence imaging

The Analyst ◽  
2018 ◽  
Vol 143 (22) ◽  
pp. 5559-5567 ◽  
Author(s):  
Thomas Söllradl ◽  
Kevin Chabot ◽  
Ulrike Fröhlich ◽  
Michael Canva ◽  
Paul G. Charette ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Fluorescence Imaging ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Individual Cell ◽  
Live Cell ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced ◽  
Signaling Activity

Validation of a combined metal-clad waveguide and surface enhanced fluorescence imaging platform for live cell imaging.

Sign in / Sign up

Export Citation Format

Share Document