scholarly journals A novel purification method for fluoride or chloride molten salts based on the redox of hydrogen on a nickel electrode

RSC Advances ◽  
2021 ◽  
Vol 11 (56) ◽  
pp. 35069-35076
Author(s):  
Yong Zuo ◽  
Yu-Long Song ◽  
Rui Tang ◽  
Yuan Qian
Keyword(s):  
Molten Salts ◽  
High Efficiency ◽  
Nickel Electrode ◽  
Purification Method

A novel and high efficiency purification method for fluoride or chloride molten salts based on the redox of hydrogen on a nickel electrode.

Study on the Extraction and Purification of Microcystins (MC-LR)

2011 ◽  
Vol 340 ◽  
pp. 318-323
Author(s):  
Wen Yi Zhang ◽  
Ning Han ◽  
Li Rong Yao ◽  
Xiao Lan Qiu ◽  
Xiao Liang Chen
Keyword(s):  
Extraction Method ◽  
Taihu Lake ◽  
High Efficiency ◽  
Extraction Efficiency ◽  
Low Cost ◽  
Solvent Concentration ◽  
Purification Method ◽  
Blue Green Algae ◽  
Extraction Solvent ◽  
Extraction And Purification

The MC-LR from the the blue-green algae of Taihu Lake was extracted, at the same time, a set of microcystins extraction method with methanol as extraction solvent and purification method with C18-SPE as purification workstations were established. The extraction solvent concentration, extraction time, extraction solvent amount, leacheate concentration and eluent concentration were used to research the extraction efficiency of MC-LR. Finally, 80% methanol was used to wash microcytins to make MC-LR high purity and the purity was over 85%. This research presented a method of low cost and high efficiency. It provided the foundation for the further research of microcytins.

scholarly journals A Novel Method of Affinity Tag Cleavage in the Purification of a Recombinant Thermostable Lipase from Aneurinibacillus thermoaerophilus Strain HZ

Catalysts ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 479 ◽  
Author(s):  
Malihe Masomian ◽  
Raja Rahman ◽  
Abu Salleh
Keyword(s):  
Organic Solvents ◽  
High Efficiency ◽  
Biochemical Characterization ◽  
Acyl Chain ◽  
Industrial Applications ◽  
Start Codon ◽  
High Yield ◽  
Medium Size ◽  
Affinity Tag ◽  
Purification Method

The development of an efficient and economical purification method is required to obtain a pure and mature recombinant protein in a simple process with high efficiency. Hence, a new technique was invented to cleave the tags from the N-terminal region of recombinant fusion HZ lipase in the absence of protease treatment. The recombinant pET32b/rHZ lipase was overexpressed into E. coli BL21 (DE3). Affinity chromatography was performed as the first step of purification. The stability of the protein was then tested in different temperatures. The fused Trx-His-S-tags to the rHZ lipase was cleaved by treatment of the fusion protein at 20 °C in 100 mM Tris-HCl buffer, pH 8.0. The precipitated tag was removed, and the mature recombinant enzyme was further characterized to specify its properties. A purification yield of 78.9% with 1.3-fold and 21.8 mg total purified mature protein was obtained from 50 mL starting a bacterial culture. N-terminal sequencing of purified recombinant HZ lipase confirmed the elimination of the 17.4 kDa tag from one amino acid before the native start codon (Methionine) of the protein. The mature rHZ lipase was highly active at 65 °C and a pH of 7.0, with a half-life of 2 h 15 min at 55 °C and 45 min at 60 °C. The rHZ lipase showed a preference for the hydrolysis of natural oil with a long carbon chain (C18) and medium-size acyl chain p-nitrophenyl esters (C10). The enzyme remained stable in the presence of nonpolar organic solvents, and its activity was increased by polar organic solvents. This study thus demonstrates a simple and convenient purification method which resulted in the high yield of mature enzyme along with unique and detailed biochemical characterization of rHZ lipase, making the enzyme favorable in various industrial applications.

scholarly journals ESTIMATION OF EFFICIENCY OF USING BRUCITE FOR DECONTAMINATION OF ARSENIC CONTAINING WATER

2019 ◽  
Vol 2 (4) ◽  
pp. 53-60
Author(s):  
Ksenia Kovalenko
Keyword(s):  
High Efficiency ◽  
Chemical Interaction ◽  
Toxic Substance ◽  
Interaction Mechanism ◽  
Chemical Processes ◽  
Sediment Stability ◽  
Mineral Surface ◽  
Purification Method ◽  
Sorption Purification ◽  
Physical And Chemical

In the paper it is outlined that mining enterprises activity is accompanied with high emissions of arsenic into the hydrosphere. Sorption purification method with application of brucite as natural mineral is proposed. As the arsenic is toxic and demand for it is limited, one of the requirements for removing of the contaminant is its collection into low-toxic and low-soluble sediments to be available for dumping. The analysis of research results is presented. The interaction mechanism of toxic substance with the sorbent is studied. It is shown that sorption includes complex of physical and chemical processes such as electrostatic interaction, ion exchange, chemical interaction of arsenic ions with mineral surface with creation hardly soluble compounds. Tests of sediment stability have been carried out by statistic method. High efficiency of using brucite for decontamination of arsenic containing water is proved.

scholarly journals High-efficiency solar thermoelectric conversion enabled by movable charging of molten salts

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chao Chang ◽  
Zongyu Wang ◽  
Benwei Fu ◽  
Yulong Ji
Keyword(s):  
Solar Energy ◽  
Thermal Energy ◽  
Molten Salts ◽  
High Efficiency ◽  
Thermal Storage ◽  
Solar Thermal ◽  
Stable Operation ◽  
Solar Thermal Energy ◽  
Thermoelectric Converter ◽  
Thermoelectric Conversion

AbstractSolar energy as an abundant renewable resource has been investigated for many years. Solar thermoelectric conversion technology, which converts solar energy into thermal energy and then into electricity, has been developed and implemented in many important fields. The operation of solar–thermal–electric conversion systems, however, is strongly affected by the intermittency of solar radiation, which requires installation of thermal storage subsystems. In this work, we demonstrated a new solar–thermal–electric conversion system that consists of a thermoelectric converter and a rapidly charging thermal storage subsystem. A magnetic-responsive solar–thermal mesh was used as the movable charging source to convert incident concentrated sunlight into high-temperature heat, which can induce solid-to-liquid phase transition of molten salts. Driven by the external magnetic field, the solar–thermal mesh can move together with the receding solid–liquid interface thus rapidly storing the harvested solar–thermal energy within the molten salts. By connecting with a thermoelectric generator, the harvested solar–thermal energy can be further converted into electricity with a solar–thermal–electric energy conversion efficiency up to 2.56%, and the converted electrical energy can simultaneously light up more than 40 orange-colored LEDs. In addition to stable operation under sunlight, the charged thermal storage subsystem can release the stored heat and thus enables the solar–thermal–electric system to continuously generate electricity after removal of solar illumination.

Ultralow Loading of Silver Nanoparticles on Mn2O3 Nanowires Derived with Molten Salts: A High-Efficiency Catalyst for the Oxidative Removal of Toluene

2015 ◽  
Vol 49 (18) ◽  
pp. 11089-11095 ◽  
Author(s):  
Jiguang Deng ◽  
Shengnan He ◽  
Shaohua Xie ◽  
Huanggen Yang ◽  
Yuxi Liu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Molten Salts ◽  
High Efficiency ◽  
Oxidative Removal

Microcalorimeters for X-Ray Spectroscopy

1988 ◽  
Vol 102 ◽  
pp. 41
Author(s):  
E. Silver ◽  
C. Hailey ◽  
S. Labov ◽  
N. Madden ◽  
D. Landis ◽  
...  
Keyword(s):  
High Resolution ◽  
High Efficiency ◽  
Thermal Response ◽  
Low Noise ◽  
High Resolution Spectroscopy ◽  
Superconducting Transition ◽  
Sapphire Substrates ◽  
Laboratory Plasmas ◽  
Adiabatic Demagnetization ◽  
Theoretical Predictions

The merits of microcalorimetry below 1°K for high resolution spectroscopy has become widely recognized on theoretical grounds. By combining the high efficiency, broadband spectral sensitivity of traditional photoelectric detectors with the high resolution capabilities characteristic of dispersive spectrometers, the microcalorimeter could potentially revolutionize spectroscopic measurements of astrophysical and laboratory plasmas. In actuality, however, the performance of prototype instruments has fallen short of theoretical predictions and practical detectors are still unavailable for use as laboratory and space-based instruments. These issues are currently being addressed by the new collaborative initiative between LLNL, LBL, U.C.I., U.C.B., and U.C.D.. Microcalorimeters of various types are being developed and tested at temperatures of 1.4, 0.3, and 0.1°K. These include monolithic devices made from NTD Germanium and composite configurations using sapphire substrates with temperature sensors fabricated from NTD Germanium, evaporative films of Germanium-Gold alloy, or material with superconducting transition edges. A new approache to low noise pulse counting electronics has been developed that allows the ultimate speed of the device to be determined solely by the detector thermal response and geometry. Our laboratory studies of the thermal and resistive properties of these and other candidate materials should enable us to characterize the pulse shape and subsequently predict the ultimate performance. We are building a compact adiabatic demagnetization refrigerator for conveniently reaching 0.1°K in the laboratory and for use in future satellite-borne missions. A description of this instrument together with results from our most recent experiments will be presented.

Uultrastructure of Microtubules

1972 ◽  
Vol 30 ◽  
pp. 252-253
Author(s):  
Ariaki Nagayama
Keyword(s):  
Fine Structure ◽  
Cultured Cells ◽  
Present Report ◽  
Confluent Monolayer ◽  
Purification Method ◽  
Vinca Rosea ◽  
L Cells ◽  
Antimitotic Activity ◽  
Monolayer Cultures ◽  
Rapid Purification

Vinblastine(Vb) or vincristine, alkaloid derived from Vinca rosea is known for its antimitotic activity by regrouping of microtubules into paracrystalline form within the cells. A rapid purification method of vinblastine-induced microtubular paracrystals(PC) has provided us with a fresh and pure microtubular material demonstrating the presence of a labile ATPase associated with the PC. The present report is concerned with the fine structure of purified microtubules of mammalian cultured cells.Confluent monolayer cultures of L cells were incubated for 20hrs with 10-5 M Vb (donated from Shionogi Seiyaku & Co., Osaka, Japan).

Imaging and diffraction modes in Scanning Transmission Electron Microscopy

1986 ◽  
Vol 44 ◽  
pp. 684-687
Author(s):  
J. M. Cowley ◽  
R. Glaisher ◽  
J. A. Lin ◽  
H.-J. Ou
Keyword(s):  
Scanning Transmission Electron Microscopy ◽  
High Efficiency ◽  
Fiber Optic ◽  
Image Intensifier ◽  
Detector System ◽  
Detector Plane ◽  
Secondary Electrons ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Special Detector

Some of the most important applications of STEM depend on the variety of imaging and diffraction made possible by the versatility of the detector system and the serial nature, of the image acquisition. A special detector system, previously described, has been added to our STEM instrument to allow us to take full advantage of this versatility. In this, the diffraction pattern in the detector plane may be formed on either of two phosphor screens, one with P47 (very fast) phosphor and the other with P20 (high efficiency) phosphor. The light from the phosphor is conveyed through a fiber-optic rod to an image intensifier and TV system and may be photographed, recorded on videotape, or stored digitally on a frame store. The P47 screen has a hole through it to allow electrons to enter a Gatan EELS spectrometer. Recently a modified SEM detector has been added so that high resolution (10Å) imaging with secondary electrons may be used in conjunction with other modes.

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