scholarly journals Transformation of Pueraria candollei var. mirifica phytoestrogens using immobilized and free β-glucosidase, a technique for enhancing estrogenic activity

RSC Advances ◽  
2021 ◽  
Vol 11 (51) ◽  
pp. 32067-32076
Author(s):  
Fonthip Makkliang ◽  
Wipawee Juengsanguanpornsuk ◽  
Suppalak Phaisan ◽  
Attapon Sakdamas ◽  
Waraporn Putalun ◽  
...  
Keyword(s):  
Estrogenic Activity ◽  
Pueraria Candollei

Transformation of Pueraria candollei var. mirifica phytoestrogens by β-glucosidase increases estrogenic activity.

scholarly journals Characterization and validation of fragment antigen-binding (Fab) antibody-based immunoassay for deoxymiroestrol, a potent phytoestrogen from Pueraria candollei

2021 ◽  
Author(s):  
Worapol Sae-foo ◽  
Supaluk Krittanai ◽  
Wipawee Juengsanguanpornsuk ◽  
Gorawit Yusakul ◽  
Tharita Kitisripanya ◽  
...  
Keyword(s):  
Quantitative Method ◽  
Estrogenic Activity ◽  
Limit Of Detection ◽  
Hormone Replacement ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Binding ◽  
Pueraria Candollei ◽  
Specific Determination ◽  
Fragment Antigen Binding

Deoxymiroestrol is the most potent phytoestrogen in chromenes group that has been found in Pueraria candollei, Thai name known as Kwao Krua Khao. Several studies reported estrogenic activity of P. candollei in order to using as hormone replacement therapy for postmenopausal women. Previously, specific determination of deoxymiroestrol content by enzyme-linked immunosorbent assay (ELISA) using polyclonal antibody (pAb) have been reported. However, production of pAb has limitation and variability from different batches. Therefore, in this study, we established quantitative method for determination of deoxymiroestrol using fragment antigen-binding (Fab) antibody-based immunoassay. The developed immunoassay has specificity to deoxymiroestrol with a calibration range of 15.6-1000 ng mL-1. Precision including intra-assay and inter-assay are 1.48-7.11 and 0.58-9.31%, respectively. Accuracy of the assay showed in recovery between 99.77-101.61% when spike deoxymiroestrol standard into the samples. The limit of detection (LOD) is 30.80 ng mL-1. Comparation antibody-based immunoassay for determination of deoxymiroestrol using Fab with pAb was represented consistency (R2 = 0.9807) when analysis roots bark of Pueraria candollei from difference areas. Therefore, this development assay can apply to determine deoxymiroestrol content in the plant samples.

scholarly journals Investigation of the estrogenic activity of Pueraria candollei variety mirifica extract on rats

2019 ◽  
Vol 8 (10) ◽  
pp. 2172
Author(s):  
Dao Thi Vui ◽  
Nguyen Thu Hang ◽  
Nguyen Quoc Huy ◽  
Bui Thanh Tung
Keyword(s):  
Estrogenic Activity ◽  
Reproductive Organs ◽  
Mature Female ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Serum Estradiol ◽  
Immature Female ◽  
Pueraria Candollei ◽  
Immature Rats ◽  
Estradiol Concentration

Background: Pueraria candollei variety mirifica (PM) has been widely used as ingredient in many rejuvenating products. In this study, we aimed to assess the estrogenic activity of PM extract grown in Vietnam.Methods: Estrogenic activity of PM extract was estimated on immature female rats by using uterotrophic method to measure the weight of the reproductive organs. Estrogenic activity of PM extract also was investigated in mature female ovariectomized rats by evaluating the vaginal cells growth, reproductive organs weight, serum estradiol concentration.Results: Our results showed that PM extract at doses of 100 mg/kg, 200 mg/kg had increased the reproductive organs weight in immature rats and female ovariectomized rats. In addition, PM extract had increased the serum estradiol concentration and the vaginal cells growth by increasing the percentage of keratinocytes in female ovariectomized rats.Conclusions: Our results showed that PM extract has strong estrogenic activity in rats.

An enzyme-linked immunoassay using anti-miroestrol monoclonal antibody for quality control of Pueraria candollei products

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
T Kitisripanya ◽  
J Komaikul ◽  
K Jutathis ◽  
O Udomsin ◽  
H Tanaka ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quality Control ◽  
Pueraria Candollei

Some Variation in Estrogenic Activity in Fresh and Dried White Clover Clones and the Ladino Variety

1960 ◽  
Vol 19 (4) ◽  
pp. 1143-1149 ◽  
Author(s):  
E. M. Bickoff ◽  
A. L. Livingston ◽  
A. N. Booth ◽  
C. R. Thompson ◽  
E. A. Hollowell ◽  
...  
Keyword(s):  
White Clover ◽  
Estrogenic Activity

Estrogenic Activity in Dehydrated and Suncured Forages

1960 ◽  
Vol 19 (1) ◽  
pp. 189-197 ◽  
Author(s):  
E. M. Bickoff ◽  
A. L. Livingston ◽  
A. N. Booth ◽  
A. P. Hendrickson ◽  
G. O. Kohler
Keyword(s):  
Estrogenic Activity

Estrogenic Activity of Soybean Forage1

1958 ◽  
Vol 17 (3) ◽  
pp. 787-791 ◽  
Author(s):  
Aden C. Magee ◽  
Gennard Matrone
Keyword(s):  
Estrogenic Activity

Stand Persistence and Estrogenic Activity of Hardseeded Subterranean Clover Cultivars for Southern and Central California

jpa ◽  
1991 ◽  
Vol 4 (1) ◽  
pp. 111-114 ◽  
Author(s):  
W. L. Graves ◽  
W. H. Weitkamp ◽  
M. R. George ◽  
G. R. Smith ◽  
B. L. Kay ◽  
...  
Keyword(s):  
Estrogenic Activity ◽  
Subterranean Clover ◽  
Central California
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