scholarly journals Amidoxime-functionalized bead cellulose for the decomposition of highly toxic organophosphates

RSC Advances ◽  
2021 ◽  
Vol 11 (29) ◽  
pp. 17976-17984
Author(s):  
Pavel Janoš ◽  
Oldřich Tokar ◽  
Marek Došek ◽  
Karel Mazanec ◽  
Petr Ryšánek ◽  
...  
Keyword(s):  
Bead Cellulose

Amidoxime-functionalized bead cellulose accelerates the cleavage of phosphoester bonds in toxic organophosphates.

Sorption of Lead by the Selective Ion Exchanger Ostsorb DETA

1992 ◽  
Vol 57 (7) ◽  
pp. 1393-1404 ◽  
Author(s):  
Ladislav Svoboda ◽  
Jan Uhlíř ◽  
Zdeněk Uhlíř
Keyword(s):  
Phosphoric Acid ◽  
Aqueous Solutions ◽  
Functional Groups ◽  
Ion Exchanger ◽  
Preconcentration Procedure ◽  
Bead Cellulose

The properties of Ostsorb DETA, a selective ion exchanger based on modified bead cellulose with chemically bonded diethylenetriamine functional groups, were studied, and its applicability to the preconcentration of trace amounts of lead from aqueous solutions was verified. The conditions of the preconcentration procedure in the column and batch modes were optimized for this purpose. The results obtained were applied to the determination of lead in phosphoric acid.

Purification of Glycerol Kinase by "Dye-Ligand" Chromatography and Hydrophobic Interaction Chromatography on Bead-Cellulose Derivatives

1993 ◽  
Vol 58 (2) ◽  
pp. 445-451 ◽  
Author(s):  
Vladimír Žúbor ◽  
Albert Breier ◽  
Marta Horváthová ◽  
Dagmar Hagarová ◽  
Peter Gemeiner ◽  
...  
Keyword(s):  
Hydrophobic Interaction ◽  
Specific Activity ◽  
Hydrophobic Interaction Chromatography ◽  
Glycerol Kinase ◽  
Enzymic Activity ◽  
Cellulose Derivatives ◽  
Long Term Storage ◽  
Bead Cellulose ◽  
Term Storage

The crude extract of cytosole enzymes was obtained from homogenized cells of Saccharomyces cerevisiae by partition. The enzyme was then isolated from the lower aqueous phase displaying higher glycerol kinase activity by dye-ligand chromatography on Cibacron Blue (CB) or Remazol Brilliant Blue R (RB)-derivatized bead-cellulose, ATP being the eluent. The specific activity of glycerol kinase rised more than 10 and 7-times after affinity dye-ligand chromatography and hydrophobic interaction chromatography, respectively. Glycerol kinase obtained by the latter method was purified by CB-bead cellulose. The final preparation maintained its enzymic activity without noticeable losses during a long-term storage at 4 °C in dark.

Partition mechanism of adsorption and the absence of displacement phenomena in the zonal analytical chromatography of proteins on bead 2-hydroxy-3-phenoxypropyl-cellulose

1989 ◽  
Vol 54 (9) ◽  
pp. 2375-2385 ◽  
Author(s):  
Peter Gemeiner ◽  
Eva Hrabárová ◽  
Magdaléna Zacharová ◽  
Albert Breier ◽  
Milan J. Beneš
Keyword(s):  
Reaction Time ◽  
Perchloric Acid ◽  
Adsorption Mechanism ◽  
Spherical Shape ◽  
Molar Ratio ◽  
Temperature Reaction ◽  
Reaction Conditions ◽  
Hydrophobic Region ◽  
Bead Cellulose ◽  
To Come

Hydrophobization of bead cellulose is described, carried out by its alkylation with 1,2-epoxy-3-phenoxypropane under the conditions of acid (perchloric acid, borontrifluoride diethyl etherate) and basic (sodium hydroxide) catalysis. Reaction conditions (temperature, reaction time, molar ratio of reactants) have been determined, allowing the hydrophobization of bead cellulose to be carried out to the largest possible extent while maintaining its spherical shape. The nonstoichiometric mechanism suggested for the adsorption of amphiphilic adsorptives on bead 2-hydroxy-3-phenoxypropyl-cellulose (HPP-C) was checked by means of adsorption of six proteins. It was found that the surface of the hydrophobic segment of the adsorbent must be sufficiently large to be able to come in touch with the hydrophobic region of the protein through its multiple residues. In such cases the partitioning of the protein between the hydrophobic segment present as a liquid-like film and the surrounding solution becomes the predominant step of the adsorption. This adsorption mechanism is also reflected in zonal chromatography on bead HPP-C, as no displacement phenomena could be observed in any of the six proteins used. Retention of these proteins has been affected to a decisive extent by the degree of hydrophobization of HPP-C.

Thiol and disulphide derivatives of cellulose

1983 ◽  
Vol 48 (1) ◽  
pp. 267-278 ◽  
Author(s):  
Peter Gemeiner ◽  
Milan Beneš
Keyword(s):  
Bead Cellulose ◽  
Acid Catalyzed ◽  
Derivatives Of

The acid catalyzed etherification of cellulose with 1-chloro-2,3-epoxypropane yielded 3-chloro-2-hydroxypropylcellulose (SCl ≤ 0.67); the latter, by a known sequence of reactions through the thiosulphate derivative and after its reduction, gave 2-hydroxy-3-mercaptopropylcellulose (up to 0.43 mmol SH/g). Mercaptodeoxycellulose (up to 0.53 mmol SH/g) was prepared from the less reactive chlorodeoxycellulose by an analogous sequence of reactions. Bead mercaptodeoxycellulose is more advantageous obtained by using tosylate of bead cellulose; this procedure is also more advantageous than the preparation of bead 2-hydroxy-3-mercaptopropylcellulose. Disulphide derivatives of cellulose were prepard (a) by a quantitative reaction of thiol derivatives with 2,2'-dipyridyl disulphide and (b) by a reversible crosslinking of cellulose or carboxymethylcellulose with bifunctional disulphides. Disulphide derivatives of cellulose prepared by procedure (b) give after reduction thiol derivatives (up to 0.185 mmol SH/g), and further by employing procedure (a) yield 2-pyridyl disulphide derivatives.

Purification of exopolygalacturonase by affinity chromatography on Concanavalin A — Bead cellulose

1996 ◽  
Vol 10 (5) ◽  
pp. 363-366
Author(s):  
E. Stratilová ◽  
D. Mislovičová ◽  
M. Dzúrová
Keyword(s):  
Affinity Chromatography ◽  
Concanavalin A ◽  
Bead Cellulose

Screening of Concanavalin A-Bead Cellulose Conjugates by an Enzyme Thermistor Using Immobilized Invertase as the Reporter Catalyst

1995 ◽  
Vol 750 (1 Enzyme Engine) ◽  
pp. 441-443 ◽  
Author(s):  
PETER DOČOLOMANSKÝ ◽  
PETER GEMEINER ◽  
DANICA MISLOVIČOVÁ ◽  
VLADIMÍR ŠTEFUCA ◽  
BENGT DANIELSSON
Keyword(s):  
Concanavalin A ◽  
Bead Cellulose ◽  
Enzyme Thermistor

Selective Sorption of Metal Oxoanions from Dilute Solution by Bead Cellulose Sorbent

2007 ◽  
Vol 42 (6) ◽  
pp. 1231-1243 ◽  
Author(s):  
Eva Mištová ◽  
Helena Parschová ◽  
Zdeněk Matějka
Keyword(s):  
Dilute Solution ◽  
Selective Sorption ◽  
Bead Cellulose
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