Laser-induced switching of the biological activity of phosphonate molecules

2021 ◽  
Author(s):  
Ilya Kolesnikov ◽  
Anastasia Khokhlova ◽  
Dmitrii Pankin ◽  
Anna Pilip ◽  
Anastasia Egorova ◽  
...  
Keyword(s):  
Biological Activity ◽  
Laser Irradiation ◽  
Phenyl Group ◽  
Effect Of Substituents ◽  
First Time ◽  
Butyrylcholinesterase Inhibition

Butyrylcholinesterase inhibition and its enhancement as a result of laser irradiation are demonstrated for the first time for a series of phosphorylated arylaminomalonates. Effect of substituents in phenyl group on...

The Pattern of Acetylation Defines the Priming Activity of Chitosan Tetramers

2019 ◽  
Author(s):  
Sven Basa ◽  
Malathi Nampally ◽  
Talita Honorato ◽  
Subha Narayan Das ◽  
Appa Rao Podile ◽  
...  
Keyword(s):  
Biological Activity ◽  
Degree Of Polymerization ◽  
Chitosan Oligosaccharides ◽  
Degree Of Acetylation ◽  
Bona Fide ◽  
First Time ◽  
Priming Activity

The biological activity of chitosans depends on their degree of polymerization (DP) and degree of acetylation (DA). However, information could also be carried by the pattern of acetylation (PA): the sequence of <i>β</i>-1,4-linked glucosamine (deacetylated/D) and <i>N</i>-acetylglucosamine (acetylated/A) units. To address this hypothesis, we prepared partially-acetylated chitosan oligosaccharides from a chitosan polymer (DA=35%, DP<sub>w</sub>=905) using recombinant chitosan hydrolases with distinct substrate and cleavage specificities. The mixtures were separated into fractions DP4–DP12, which were tested for elicitor and priming activities in rice cells. We confirmed that both activities were influenced by DP, <a>but also observed apparent DA-dependent priming activity, with the ADDD+DADD fraction proving remarkably effective</a>. We then compared all four mono-acetylated tetramers prepared using different chitin deacetylases and observed significant differences in priming activity. This demonstrates for the first time that PA influences the biological activity of chitosans, which can now be recognized as <i>bona fide</i> information-carrying molecules

The Pattern of Acetylation Defines the Priming Activity of Chitosan Tetramers

2019 ◽  
Author(s):  
Sven Basa ◽  
Malathi Nampally ◽  
Talita Honorato ◽  
Subha Narayan Das ◽  
Appa Rao Podile ◽  
...  
Keyword(s):  
Biological Activity ◽  
Degree Of Polymerization ◽  
Chitosan Oligosaccharides ◽  
Degree Of Acetylation ◽  
Bona Fide ◽  
First Time ◽  
Priming Activity

The biological activity of chitosans depends on their degree of polymerization (DP) and degree of acetylation (DA). However, information could also be carried by the pattern of acetylation (PA): the sequence of <i>β</i>-1,4-linked glucosamine (deacetylated/D) and <i>N</i>-acetylglucosamine (acetylated/A) units. To address this hypothesis, we prepared partially-acetylated chitosan oligosaccharides from a chitosan polymer (DA=35%, DP<sub>w</sub>=905) using recombinant chitosan hydrolases with distinct substrate and cleavage specificities. The mixtures were separated into fractions DP4–DP12, which were tested for elicitor and priming activities in rice cells. We confirmed that both activities were influenced by DP, <a>but also observed apparent DA-dependent priming activity, with the ADDD+DADD fraction proving remarkably effective</a>. We then compared all four mono-acetylated tetramers prepared using different chitin deacetylases and observed significant differences in priming activity. This demonstrates for the first time that PA influences the biological activity of chitosans, which can now be recognized as <i>bona fide</i> information-carrying molecules

Strategies for Oral Delivery of Metal-Saturated Lactoferrin

2019 ◽  
Vol 20 (11) ◽  
pp. 1046-1051 ◽  
Author(s):  
Przemysław Gajda-Morszewski ◽  
Klaudyna Śpiewak-Wojtyła ◽  
Maria Oszajca ◽  
Małgorzata Brindell
Keyword(s):  
Biological Activity ◽  
Oral Delivery ◽  
Therapeutic Potential ◽  
Structure And Function ◽  
The Difference ◽  
And Function ◽  
First Time

Lactoferrin was isolated and purified for the first time over 50-years ago. Since then, extensive studies on the structure and function of this protein have been performed and the research is still being continued. In this mini-review we focus on presenting recent scientific efforts towards the elucidation of the role and therapeutic potential of lactoferrin saturated with iron(III) or manganese(III) ions. The difference in biological activity of metal-saturated lactoferrin vs. the unmetalated one is emphasized. The strategies for oral delivery of lactoferrin, are also reviewed, with particular attention to the metalated protein.

scholarly journals In vitro biological activity of Hydroclathrus clathratus and its use as an extracellular bioreductant for silver nanoparticle formation

2020 ◽  
Vol 9 (1) ◽  
pp. 416-428 ◽  
Author(s):  
Raghad R. Alzahrani ◽  
Manal M. Alkhulaifi ◽  
Nouf M. Al-Enazi
Keyword(s):  
Biological Activity ◽  
Unsaturated Fatty Acids ◽  
Multidrug Resistant ◽  
Chemical Components ◽  
Resistant Bacteria ◽  
Transmission Electron ◽  
Hydroclathrus Clathratus ◽  
Adaptive Nature ◽  
First Time

AbstractThe adaptive nature of algae results in producing unique chemical components that are gaining attention due to their efficiency in many fields and abundance. In this study, we screened the phytochemicals from the brown alga Hydroclathrus clathratus and tested its ability to produce silver nanoparticles (AgNPs) extracellularly for the first time. Lastly, we investigated its biological activity against a variety of bacteria. The biosynthesized nanoparticles were characterized by UV-visible spectroscopy, Fourier-transform infrared spectroscopy, dynamic light scattering, transmission electron microscopy, and energy-dispersive spectroscopy. The biological efficacy of AgNPs was tested against eighteen different bacteria, including seven multidrug-resistant bacteria. Phytochemical screening of the alga revealed the presence of saturated and unsaturated fatty acids, sugars, carboxylic acid derivatives, triterpenoids, steroids, and other components. Formed AgNPs were stable and ranged in size between 7 and 83 nm and presented a variety of shapes. Acinetobacter baumannii, Staphylococcus aureus, Methicillin-resistant S. aureus (MRSA), and MDR A. baumannii were the most affected among the bacteria. The biofilm formation and development assay presented a noteworthy activity against MRSA, with an inhibition percentage of 99%. Acknowledging the future of nano-antibiotics encourages scientists to explore and enhance their potency, notably if they were obtained using green, rapid, and efficient methods.

Synthesis of Galactomannan Sulfate-Citrate

2022 ◽  
Vol 1049 ◽  
pp. 218-223
Author(s):  
Aleksandr S. Kazachenko ◽  
Yuriy N. Malyar ◽  
Anna S. Kazachenko
Keyword(s):  
Biological Activity ◽  
Ftir Spectroscopy ◽  
X Ray Diffraction ◽  
Absorption Bands ◽  
X Ray ◽  
Mixed Ester ◽  
First Time ◽  
Derivatives Of

Sulfated derivatives of polysaccharides have anticoagulant, hypolipedimic and other biological activity. In this work, a complex mixed ester of galactomannan, its sulfate-citrate, was obtained for the first time. The introduction of citrate and sulfate groups was proved by FTIR spectroscopy by the appearance of corresponding absorption bands. It was shown by X-ray diffraction that the introduction of the citrate group leads to the amorphization of the galactomannan structure.

scholarly journals Plasmonic photothermal microneedle arrays and single needles for minimally-invasive deep in-skin hyperthermia

2020 ◽  
Vol 8 (25) ◽  
pp. 5425-5433 ◽  
Author(s):  
Álvaro Cárcamo-Martínez ◽  
Brónach Mallon ◽  
Juan Domínguez-Robles ◽  
A. Sara Cordeiro ◽  
Maurizio Celentano ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Laser Irradiation ◽  
Gold Nanorods ◽  
Near Infrared ◽  
Skin Model ◽  
Thickness Skin ◽  
Microneedle Arrays ◽  
First Time ◽  
Nir Laser ◽  
Deep Hyperthermia

We report, for the first time, crosslinked polymeric microneedle (MN) arrays and single needles (2 mm and 4.5 mm length) coated with gold nanorods (GnRs) to induce deep hyperthermia in a 3 mm-thickness skin model upon near infrared (NIR) laser irradiation.

scholarly journals Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Christiane Schineider Machado ◽  
João Benhur Mokochinski ◽  
Tatiana Onofre de Lira ◽  
Fátima de Cassia Evangelista de Oliveira ◽  
Magda Vieira Cardoso ◽  
...  
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Quality Standard ◽  
Antimicrobial Activities ◽  
Mato Grosso ◽  
Gram Positive Bacteria ◽  
H Nmr ◽  
Chemical Profiles ◽  
Brazilian Propolis ◽  
First Time

The chemical composition and biological activity of a sample of yellow propolis from Mato Grosso do Sul, Brazil (EEP-Y MS), were investigated for the first time and compared with green, brown, and red types of Brazilian propolis and with a sample of yellow propolis from Cuba. Overall, EEP-Y MS had different qualitative chemical profiles, as well as different cytotoxic and antimicrobial activities when compared to the other types of propolis assessed in this study and it is a different chemotype of Brazilian propolis. Absence of phenolic compounds and the presence of mixtures of aliphatic compounds in yellow propolis were determined by analysing1H-NMR spectra and fifteen terpenes were identified by GC-MS. EEP-Y MS showed cytotoxic activity against human tumour strain OVCAR-8 but was not active against Gram-negative or Gram-positive bacteria. Our results confirm the difficulty of establishing a uniform quality standard for propolis from diverse geographical origins. The most appropriate pharmacological applications of yellow types of propolis must be further investigated.

The Synthesis and Biological Evaluation of Anithiactin A/Thiasporine C and Analogues

2015 ◽  
Vol 68 (12) ◽  
pp. 1829 ◽  
Author(s):  
Richard A. Lamb ◽  
Michael P. Badart ◽  
Brooke E. Swaney ◽  
Sinan Gai ◽  
Sarah K. Baird ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Biological Activity ◽  
Cell Lines ◽  
Breast Cancer Cell ◽  
Phenyl Group ◽  
Biological Evaluation ◽  
Breast Cancer Cell Lines ◽  
High Concentration ◽  
Structural Requirements ◽  
Synthesis And Biological Evaluation

The synthesis of anithiactin A has been achieved in four steps. Several closely related analogues were synthesised and their biological activity against colon and breast cancer cell lines evaluated. Anithiactin A was found not to be cytotoxic even at a high concentration (100 μM); however, two 4-substituted phenyl thiazoles were found to be moderately cytotoxic at 10 μM. Based on these results, 4-substitution on the phenyl group appears to be critical for cytotoxicity. However, the exact electronic and structural requirements are unclear.

scholarly journals Recovery of pure C-phycoerythrin from a limestone drought tolerant cyanobacterium Nostoc sp. and evaluation of its biological activity

2020 ◽  
pp. 115-128
Author(s):  
Bahareh Nowruzi ◽  
Hossein Fahimi ◽  
Adriana Sturion Lorenzi
Keyword(s):  
Biological Activity ◽  
Antifungal Agents ◽  
Extreme Environments ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Biotechnological Applications ◽  
Drought Tolerant ◽  
Drought Conditions ◽  
Antibacterial And Antifungal ◽  
First Time

Se realizó la caracterización de ficoeritrina de la cepa A5 de Nostoc sp., seguida de investigación de su actividad biológica para aplicaciones biotecnológicas. Para la extracción de ficoeritrina, el uso de tampón acetato (pH 5.1) produjo 65.04 µg mL-1, y se identificó como C-ficoeritrina. Los resultados de su actividad antioxidante sugirieron su acción como un potente eliminador de radicales libres. Además, la C-ficoeritrina de Nostoc mostró una capacidad notable como agente antibacteriano y antifúngico, con estabilidad significativa de hasta 10 días. La glucosa (4 mg mL-1) fue un buen conservante para la C-ficoeritrina a 25 y 4 ºC. Se obtuvo por primera vez una C-ficoeritrina estable de Nostoc sp. en condiciones de sequía en piedra caliza, lo que demuestra la necesidad de estudiar microorganismos de ambientes extremos. The phycoerythrin characterization from Nostoc sp. strain A5 was done, followed by investigation of its biological activity for biotechnological applications. For phycoerythrin extraction, the use of acetate buffer (pH 5.1) resulted in 65.04 µg mL-1, and C-phycoerythrin was identified. Results of its antioxidant activity suggested action as a potent free radical scavenger. In addition, Nostoc's C-phycoerythrin showed noteworthy ability for antibacterial and antifungal agents with significant stability up to 10 days. Glucose (4 mg mL-1) was a good preservative for C-phycoerythrin at 25 and 4 ºC. A stable C-phycoerythrin from Nostoc sp. was obtained for the first time from limestone drought conditions, showing the need of studying microorganisms from extreme environments.

STEROIDS PRESENT IN THE FOLLICULAR FLUID OF THE MARE

1960 ◽  
Vol 20 (2) ◽  
pp. 147-156 ◽  
Author(s):  
R. V. SHORT
Keyword(s):  
Biological Activity ◽  
Natural Product ◽  
Follicular Fluid ◽  
Physiological Significance ◽  
Chromatographic Techniques ◽  
First Time

SUMMARY Using paper chromatographic techniques, it has been possible to identify the following steroids in the follicular fluid of the mare (results expressed as μg/1.): oestradiol-17β, 460 μg; oestrone, 34 μg; cortisol, 12 μg; progesterone, 124 μg; 17α-hydroxyprogesterone, 66 μg; epitestosterone, 24 μg; androstenedione, 136 μg. In addition to these seven compounds, 136 μg of an unidentified oestrogen-like substance and 52 μg of an unidentified 17-ketosteroid were also present. This is the first time that epitestosterone has been identified as a natural product, and the possible significance of this compound is discussed. 19-Hydroxyandrostenedione, the postulated intermediate in the formation of oestrone from androstenedione, was not detected in this study, and some doubt is therefore cast on the theory that it is an essential intermediate in the biosynthesis of the ovarian oestrogens. The presence of relatively large amounts of an unidentified polar oestrogen in follicular fluid suggests that it may be of some physiological significance, although at the present time no information is available on the biological activity of this compound.

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