Inertial Focusing of Circulating Tumor Cells in Whole Blood at High Flow Rates using the Microfluidic CTCKey™ Device for CTC Enrichment

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Kaylee Judith Smith ◽  
Jessica Antoinette Jana ◽  
Anna Kaehr ◽  
Emma Purcell ◽  
Tyler Opdycke ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Treatment Decisions ◽  
Blood Stream ◽  
High Flow ◽  
Flow Rates ◽  
Inertial Focusing ◽  
Direct Treatment ◽  
Rare Cells

Circulating Tumor Cells (CTCs) are extremely rare cells shed from tumors into the blood stream. These cells can provide valuable information about their tumor of origin and direct treatment decisions...

scholarly journals Chemically modified plastic tube for high volume removal and collection of circulating tumor cells

2015 ◽  
Author(s):  
Angelo Gaitas ◽  
Gwangseong Kim
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
High Volume ◽  
Blood Stream ◽  
Plastic Tube ◽  
Chemically Modified ◽  
Stage Of Development ◽  
Flow Through

In this preliminary effort, we use a commercially available and chemically modified tube to selectively capture circulating tumor cells (CTCs) from the blood stream by immobilizing human anti-EpCAM antibodies on the tube's interior surface. We describe the steps required to modify a tube into a cancer cell capturing device. Using these simple modifications, at this proof-of-concept stage of development, we were able to capture about 85% of cancer cells from suspension and 44% of cancer cells from spiked whole blood, the capture percentage being dependent on the tube's length and the number of cancer cells present. Previous work by other researchers has focused on extracting small blood volumes and capturing CTCs with complicated micro-fluidic devices for diagnostic purposes. In addition, prior results of other researchers point to a possible reduction in metastasis achieved by removing CTCs from the bloodstream. We believe that with the utilization of appropriate tube lengths and procedures, we can ensure capture and removal of nearly the entire CTC population in whole blood. Following whole blood flow through the tube, the tube can be trypsinized to release the captured live CTCs for further analysis and testing.

A two-dimensional immunomagnetic nano-net for the efficient isolation of circulating tumor cells in whole blood

Nanoscale ◽  
2019 ◽  
Vol 11 (44) ◽  
pp. 21119-21127 ◽  
Author(s):  
Chian-Hui Lai ◽  
Wen-Sy Tsai ◽  
Muh-Hwa Yang ◽  
Teh-Ying Chou ◽  
Ying-Chih Chang
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Two Dimensional ◽  
Rare Cells

An immunomagnetic morphable “nano-net” was designed and synthesized for specifically capturing rare cells of interest from mixtures.

scholarly journals Chemically modified plastic tube for high volume removal and collection of circulating tumor cells

2015 ◽  
Author(s):  
Angelo Gaitas ◽  
Gwangseong Kim
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
High Volume ◽  
Blood Stream ◽  
Plastic Tube ◽  
Chemically Modified ◽  
Stage Of Development ◽  
Flow Through

In this preliminary effort, we use a commercially available and chemically modified tube to selectively capture circulating tumor cells (CTCs) from the blood stream by immobilizing human anti-EpCAM antibodies on the tube's interior surface. We describe the steps required to modify a tube into a cancer cell capturing device. Using these simple modifications, at this proof-of-concept stage of development, we were able to capture about 85% of cancer cells from suspension and 44% of cancer cells from spiked whole blood, the capture percentage being dependent on the tube's length and the number of cancer cells present. Previous work by other researchers has focused on extracting small blood volumes and capturing CTCs with complicated micro-fluidic devices for diagnostic purposes. In addition, prior results of other researchers point to a possible reduction in metastasis achieved by removing CTCs from the bloodstream. We believe that with the utilization of appropriate tube lengths and procedures, we can ensure capture and removal of nearly the entire CTC population in whole blood. Following whole blood flow through the tube, the tube can be trypsinized to release the captured live CTCs for further analysis and testing.

A dielectrophoresis-based microfluidic chip for trapping circulating tumor cells using a porous membrane

2021 ◽  
Author(s):  
Malihe Farasat ◽  
Maede Chavoshi ◽  
Atin Bakhshi ◽  
Aref Valipour ◽  
Majid Badieirostami
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Chip ◽  
Large Cell ◽  
Blood Stream ◽  
Porous Membrane ◽  
Novel Biomarkers ◽  
Rare Cells ◽  
On Chip ◽  
Trapping Performance

Abstract Circulating tumor cells (CTCs) have been widely considered as novel biomarkers for clinical diagnosis of cancer. CTCs are the cells detached from the parent tumors and shed into the blood stream to initiate tumor metastasis. Although CTCs are rare, their detection in one’s blood sample is essential for cancer early diagnosis and for starting the treatment procedure. Here, we introduce a novel method for trapping CTCs using dielectrophoresis (DEP), which effectively employs pores of a replaceable porous membrane as CTC traps. The applied dielectrophoretic force efficiently traps and holds CTCs in a stable position and further enables us to perform various on chip analysis on them. First, using finite element method, the performance of the system was simulated for different physical conditions. Then, the chip was fabricated and its trapping performance was experimentally validated. Cells were entered into the microchannel and trapped in the pores of a polydimethylsiloxane (PDMS) membrane. The proposed microfluidic chip is capable of detecting rare cells in a large cell population.

A Microfluidic Platform for On-Chip Analysis of Circulating Tumor Cells

2021 ◽  
Author(s):  
Jeff Darabi ◽  
Joseph Schober
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Automated Image Analysis ◽  
Rare Cancer ◽  
On Chip ◽  
Chip Analysis ◽  
Selection Of

Abstract Studies have shown that primary tumor sites begin shedding cancerous cells into peripheral blood at early stages of cancer, and the presence and frequency of circulating tumor cells (CTCs) in blood is directly proportional to disease progression. The challenge is that the concentration of the CTCs in peripheral blood may be extremely low. In the past few years, several microfluidic-based concepts have been investigated to isolate CTCs from whole blood. However, these devices are generally hampered by complex fabrication processes and very low volumetric throughputs, which may not be practical for rapid clinical applications. This paper presents a high-performance yet simple magnetophoretic microfluidic chip for the enrichment and on-chip analysis of rare CTCs from blood. Microscopic and flow cytometric assays developed for selection of cancer cell lines, selection of monoclonal antibodies, and optimization of bead coupling are discussed. Additionally, on-chip characterization of rare cancer cells using high resolution immunofluorescence microscopy and modeling results for prediction of CTC capture length are presented. The device has the ability to interface directly with on-chip pre and post processing modules such as mixing, incubation, and automated image analysis systems. These features will enable us to isolate rare cancer cells from whole blood and detect them on the chip with subcellular resolution.

Ex VivoDetection of Circulating Tumor Cells from Whole Blood by Direct Nanoparticle Visualization

ACS Nano ◽  
2018 ◽  
Vol 12 (2) ◽  
pp. 1902-1909 ◽  
Author(s):  
Regivaldo G. Sobral-Filho ◽  
Lindsay DeVorkin ◽  
Sarah Macpherson ◽  
Andrew Jirasek ◽  
Julian J. Lum ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood
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