Ex VivoDetection of Circulating Tumor Cells from Whole Blood by Direct Nanoparticle Visualization

ACS Nano ◽  
2018 ◽  
Vol 12 (2) ◽  
pp. 1902-1909 ◽  
Author(s):  
Regivaldo G. Sobral-Filho ◽  
Lindsay DeVorkin ◽  
Sarah Macpherson ◽  
Andrew Jirasek ◽  
Julian J. Lum ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood

A Microfluidic Platform for On-Chip Analysis of Circulating Tumor Cells

2021 ◽  
Author(s):  
Jeff Darabi ◽  
Joseph Schober
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Automated Image Analysis ◽  
Rare Cancer ◽  
On Chip ◽  
Chip Analysis ◽  
Selection Of

Abstract Studies have shown that primary tumor sites begin shedding cancerous cells into peripheral blood at early stages of cancer, and the presence and frequency of circulating tumor cells (CTCs) in blood is directly proportional to disease progression. The challenge is that the concentration of the CTCs in peripheral blood may be extremely low. In the past few years, several microfluidic-based concepts have been investigated to isolate CTCs from whole blood. However, these devices are generally hampered by complex fabrication processes and very low volumetric throughputs, which may not be practical for rapid clinical applications. This paper presents a high-performance yet simple magnetophoretic microfluidic chip for the enrichment and on-chip analysis of rare CTCs from blood. Microscopic and flow cytometric assays developed for selection of cancer cell lines, selection of monoclonal antibodies, and optimization of bead coupling are discussed. Additionally, on-chip characterization of rare cancer cells using high resolution immunofluorescence microscopy and modeling results for prediction of CTC capture length are presented. The device has the ability to interface directly with on-chip pre and post processing modules such as mixing, incubation, and automated image analysis systems. These features will enable us to isolate rare cancer cells from whole blood and detect them on the chip with subcellular resolution.

scholarly journals Hybrid negative enrichment of circulating tumor cells from whole blood in a 3D-printed monolithic device

Lab on a Chip ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 3427-3437 ◽  
Author(s):  
Chia-Heng Chu ◽  
Ruxiu Liu ◽  
Tevhide Ozkaya-Ahmadov ◽  
Mert Boya ◽  
Brandi E. Swain ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Whole Blood ◽  
3D Printed

A monolithic 3D-printed microfluidic device integrated with stacked layers of functionalized leukodepletion channels and microfiltration for the negative enrichment of circulating tumor cells directly from clinically relevant volumes of whole blood.

Abstract 511: Unbiased enrichment of circulating tumor cells directly from whole blood

2016 ◽  
Author(s):  
Carrie E. Peters ◽  
Danay Maestre-Battle ◽  
Steven M. Woodside ◽  
Terry E. Thomas ◽  
Allen C. Eaves
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood

Size-matching hierarchical micropillar arrays for detecting circulating tumor cells in breast cancer patients’ whole blood

Nanoscale ◽  
2019 ◽  
Vol 11 (14) ◽  
pp. 6677-6684 ◽  
Author(s):  
Zhongshun Wang ◽  
Daren Xu ◽  
Xuekui Wang ◽  
Yue Jin ◽  
Bo Huo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Breast Cancer Patients ◽  
Micropillar Array ◽  
Micropillar Arrays

A size-matching hierarchical micropillar array is fabricated for detecting circulating tumor cells in breast cancer patients’ whole blood.

Mechanical Low-Pass Filtering of Cells for Detection of Circulating Tumor Cells in Whole Blood

2020 ◽  
Vol 92 (3) ◽  
pp. 2483-2491 ◽  
Author(s):  
Taiki Suzuki ◽  
Noritada Kaji ◽  
Hirotoshi Yasaki ◽  
Takao Yasui ◽  
Yoshinobu Baba
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Low Pass
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