Cascaded filter deterministic lateral displacement microchips for isolation and molecular analysis of circulating tumor cells and fusion cells

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Zongbin Liu ◽  
Yuqing Huang ◽  
Wenli Liang ◽  
Jing Bai ◽  
Hongtao Feng ◽  
...  
Keyword(s):  
Cancer Research ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Molecular Analysis ◽  
Lateral Displacement ◽  
Personalized Treatment ◽  
Blood Samples ◽  
Deterministic Lateral Displacement ◽  
Considerable Potential ◽  
Cascaded Filter

Precise isolation and analysis of circulating tumor cells (CTCs) from blood samples offer considerable potential for cancer research and personalized treatment. Currently, available CTCs isolation approaches remain challenging in the...

scholarly journals Numerical Simulation of Separation of Circulating Tumor Cells from Blood Stream in Deterministic Lateral Displacement (DLD) Microfluidic Channel

2015 ◽  
Vol 32 (4) ◽  
pp. 463-471 ◽  
Author(s):  
F. Khodaee ◽  
S. Movahed ◽  
N. Fatouraee ◽  
F. Daneshmand
Keyword(s):  
Fluid Flow ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Flow Condition ◽  
Lateral Displacement ◽  
Microfluidic Devices ◽  
Blood Stream ◽  
Deterministic Lateral Displacement ◽  
Effective Design

AbstractDeterministic Lateral Displacement (DLD) microfluidic devices provide a reliable label-free separation method for detection of circulating tumor cells (CTCs) in blood samples based on their biophysical properties. In this paper, we proposed an effective design of the DLD microfluidic device for the CTC separation in the blood stream. A typical DLD array is designed and numerical simulations are performed to separate the CTC and leukocyte (white blood cells) in different fluid flow conditions. Fluid-Solid Interaction method is used to investigate the behaviour of these deformable cells in fluid flow. In this study, the effects of critical parameters affecting cell separation in the DLD microfluidic devices (e.g.flow condition, cell deformability, and stress) have been investigated. The obtained results show that unlike leukocytes, the CTC’s motion is independent of the flow condition and is laterally displaced even in higher Reynolds number. Larger cells (CTCs) cannot intercept the low-velocity fluid near the wall of the posts; thus, they move faster and become separated from leukocytes. To reduce the cellular stress during separation process, which causes increase of cell viability and more effective design of microfluidic device, the results obtained here may be used as a significant design parameter for the DLD fabrication.

Mitigation of Channel Clogging in a Microfluidic Device for Capturing Circulating Tumor Cells

2020 ◽  
Vol 14 (1) ◽  
pp. 109-116
Author(s):  
Tomoki Konishi ◽  
Yuki Jingu ◽  
Tatsuya Yoshizawa ◽  
Masaru Irita ◽  
Toshihiro Suzuki ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Propidium Iodide ◽  
Peripheral Blood ◽  
Lateral Displacement ◽  
Microfluidic Devices ◽  
Cell Detachment ◽  
Deterministic Lateral Displacement ◽  
Blood Specimen

Deterministic lateral displacement (DLD) based microfluidic devices have been developed for capturing circulating tumor cells (CTCs) from the peripheral blood. There was frequent and problematic channel clogging around the micro-post array formed on a microchannel of the device. In this study, various agents were dispersed into the blood specimen to avoid clogging. At first, platelet aggregation was considered to be the cause of the clogging, but even plasmin, which was assumed to decompose platelet aggregations, did not show obvious inhibition of the clogging. Then, enzymes used for cell detachment from tissue were examined and decomposition of the clogging residue was observed. Finally, dispersion of deoxyribonuclease into a blood specimen was found to be effective for the inhibition of clogging. The existence of DNA in the clogging residue was also confirmed by propidium iodide (PI) staining, suggesting DNA adhering to the micro-post.

Enhancing the Cell Viability in High Throughput Deterministic Lateral Displacement Separation of Circulating Tumor Cells

2019 ◽  
Author(s):  
Arian Aghilinejad ◽  
Christopher Landry ◽  
George Cha ◽  
Xiaolin Chen
Keyword(s):  
Reynolds Number ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Cancer Metastasis ◽  
Lateral Displacement ◽  
Shear Stresses ◽  
Label Free ◽  
Deterministic Lateral Displacement ◽  
Reynolds Number Flow

Abstract Cancer is among a major health concerns all over the world. Cancer metastasis, which defines as the migration of malignant cells from original sites to distant organs, is the main reason of death due to cancer and there is growing evidence that Circulating Tumor Cells (CTCs) are responsible for initiating the metastasis. Due to the importance of these bioparticles in biotechnology and medicine, there is a growing interest to study and separate them through different techniques especially microfluidic label-free technologies. One such technology, termed Deterministic Lateral Displacement (DLD) has recently shown promising abilities to separate cells and particles of different sizes. However, DLD is a separation method that takes advantages of the predictable flow laminae of low Reynolds number (Re) fluid flow. In order to achieve higher device throughput, effects of higher Reynolds number flow on DLD device should be studied. Additionally, the higher flow rates would apply higher forces and shear stresses on the cells which threaten the cell’s viability. In this study, employing numerical simulation, the effect of high Re number on DLD device for separating cancer cells has been investigated. Specifically, we conducted force analysis and by focusing on the downstream gap distance between the posts, we improved the device which results in less cell deformation. Our developed numerical model and presented results lay the groundwork for design and fabrication of high-throughput DLD microchips for enhanced separation of CTCs.

Detection of circulating tumor cells based on improved SERS-active magnetic nanoparticles

2019 ◽  
Vol 11 (22) ◽  
pp. 2918-2928 ◽  
Author(s):  
Ting Xue ◽  
Siqi Wang ◽  
Guoyu Ou ◽  
Yong Li ◽  
Huimin Ruan ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Molecular Analysis ◽  
Cell Expansion ◽  
Magnetic Nanoparticle ◽  
Blood Samples ◽  
Phenotype Identification

A SERS-active magnetic nanoparticle was developed for capture, enrichment and detection of cancer cells from blood samples. The isolated CTCs can also be released for further cell expansion, phenotype identification and molecular analysis.

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