Gram-scale production of sugar nucleotides and their derivatives

2021 ◽  
Author(s):  
Shuang Li ◽  
shuaishuai wang ◽  
Yaqian Wang ◽  
Jingyao Qu ◽  
Xianwei Liu ◽  
...  
Keyword(s):  
Scale Production ◽  
Purification Process ◽  
Selective Precipitation ◽  
Sugar Nucleotides ◽  
Production Strategy

Here, we report a practical sugar nucleotides production strategy that combined high-concentrated multienzyme catalyzed reaction and a robust chromatography-free selective precipitation purification process. Twelve sugar nucleoitdes were synthesized at gram-scale...

scholarly journals Pilot-Scale Production of Chito-Oligosaccharides Using an Innovative Recombinant Chitosanase Preparation Approach

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 290
Author(s):  
Chih-Yu Cheng ◽  
Chia-Huang Tsai ◽  
Pei-Jyun Liou ◽  
Chi-Hang Wang
Keyword(s):  
Cell Density ◽  
Scale Up ◽  
Cost Effective ◽  
Pilot Scale ◽  
Ionic Concentration ◽  
Dihydrogen Phosphate ◽  
Scale Production ◽  
Sodium Dihydrogen Phosphate ◽  
Selective Precipitation ◽  
Pilot Scale Production

For pilot-scale production of chito-oligosaccharides, it must be cost-effective to prepare designable recombinant chitosanase. Herein, an efficient method for preparing recombinant Bacillus chitosanase from Escherichia coli by elimination of undesirable substances as a precipitate is proposed. After an optimized culture with IPTG (Isopropyl β-d-1-thiogalactopyranoside) induction, the harvested cells were resuspended, disrupted by sonication, divided by selective precipitation, and stored using the same solution conditions. Several factors involved in these procedures, including ion types, ionic concentration, pH, and bacterial cell density, were examined. The optimal conditions were inferred to be pH = 4.5, 300 mM sodium dihydrogen phosphate, and cell density below 1011 cells/mL. Finally, recombinant chitosanase was purified to >70% homogeneity with an activity recovery and enzyme yield of 90% and 106 mg/L, respectively. When 10 L of 5% chitosan was hydrolyzed with 2500 units of chitosanase at ambient temperature for 72 h, hydrolyzed products having molar masses of 833 ± 222 g/mol with multiple degrees of polymerization (chito-dimer to tetramer) were obtained. This work provided an economical and eco-friendly preparation of recombinant chitosanase to scale up the hydrolysis of chitosan towards tailored oligosaccharides in the near future.

scholarly journals IgY extraction and purification from chicken egg yolk

2018 ◽  
Vol 68 (3) ◽  
pp. 265 ◽  
Author(s):  
G BIZANOV
Keyword(s):  
Polyethylene Glycol ◽  
Egg Yolk ◽  
Water Soluble ◽  
Dilution Method ◽  
Scale Production ◽  
Immunoglobulin Y ◽  
Selective Precipitation ◽  
Isolation And Purification ◽  
Enteric Diseases ◽  
Water Dilution

The interest for immunoglobulin Y (IgY) isolation arises from the possible applications of these immunoglobulins in diagnostics and therapeutics. Powered whole eggs or yolks have been used in veterinary medicine as an inexpensive immunoglobulin Y source for the treatment of enteric diseases. Incorporating feed grade egg yolk antibodies into animal diets has been examined extensively in an attempt to limit pathogenic diarrhea causing by Escherichia coli in swine, and limit Salmonella establishment in calves and mice, as well as Campylobacter, Clostridium, and Salmonella in poultry. Thus, therapeutic administration of the IgY might reduce the clinical use of antibiotics, and minimize the risk of developing bacterial antibiotic resistance. Methods of immunoglobulins isolation and purification from hen yolk are reviewed. For a large-scale production, one of the problems is separating the water-soluble protein fraction from the lipids and other hydrophobic substances. Precipitation or aggregation of lipid occurs under various conditions: (1) by use of water dilution under acidic conditions; (2) by means of acids; (3) combination of solvents (chloroform, aceton) which selectively solubilize the lipids; (4) using of 3.5% polyethylene glycol and (5) by applying natural gums (polyanionic polysaccharides). Further purification of IgY after crude extraction can be achieved by selective precipitation. IgY precipitation can be carried out by salt precipitation using saturated solutions of sodium or ammonium sulphate, which dehydrate proteins. Furthermore, selective precipitation is achieved by using 8.8% sodium chloride or at 12% of polyethylene glycol. The methods comparison on the basis of IgY yield, showed that the mean IgY yield obtained by water dilution method or precipitation with salts, or by using polyethylene glycol was 5.6 mg/ml of egg yolk, 6.3 mg/ml of egg yolk and 8.7 mg/ml of egg yolk correspondingly and did not significantly differ between them. Water dilution method seemed to offer the best IgY recoveries. Moreover, this method in combination with chromatography and filtration can be applied easily in an industrial environment. Filtration technology offers the best opportunities for industrial applications while precipitation with polyethylene glycol or salts provides a cheap and easy methodology for laboratory use. In the production of safer foods, an important strategy is to exploit natural antimicrobial agents as alternatives to conventional synthetic chemical preservatives. In this regard, the IgY is of much interest for its potential application in fortified foods, such as administration for prevention of enteric diseases. The use of IgY is cost-effective.

How to Develop Quality Courseware: Results of a Survey

1988 ◽  
Vol 16 (4) ◽  
pp. 323-335
Author(s):  
Urs Karrer
Keyword(s):  
Large Scale ◽  
Production Systems ◽  
The United States ◽  
Strong Impact ◽  
Scale Production ◽  
Federal Republic Of Germany ◽  
Quality Factors ◽  
Large Scale Production ◽  
Production Strategy

A research project was started in 1985 to explore large-scale production systems which have a strong impact on the development of quality courseware. The exploration and evaluation of these production systems contribute to the explanation of the overall unsatisfactory quality of courseware. This article focuses on results of a survey which was conducted in January 1987 addressing more than sixty profit and nonprofit institutions in England, the federal Republic of Germany, the Netherlands, Switzerland, and the United States. The survey revealed interesting results in various fields. The five working hypotheses (production strategy, production approach, and quality factors for courseware development) were confirmed to a great extent. These results may be instructional for institutions which recently joined this area and/or are planning to do so.

Development of a novel adenovirus purification process utilizing selective precipitation of cellular DNA

2005 ◽  
Vol 91 (1) ◽  
pp. 12-21 ◽  
Author(s):  
Aaron R. Goerke ◽  
Brian C.S. To ◽  
Ann L. Lee ◽  
Sangeetha L. Sagar ◽  
John O. Konz
Keyword(s):  
Purification Process ◽  
Selective Precipitation ◽  
Cellular Dna

Analysis of galanthamine and related alkaloids in plant extracts and during the purification process by GC-MS

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
S Berkov ◽  
C Codina ◽  
F Viladomat ◽  
J Bastida
Keyword(s):  
Plant Extracts ◽  
Purification Process

PENGARUH PELARUT KLOROFORM DALAM PEMURNIAN GLISEROL DENGAN PROSES ASIDIFIKASI ASAM KLORIDA

2016 ◽  
Vol 5 (3) ◽  
pp. 38-43
Author(s):  
Windi Monica Surbakti ◽  
Gerson Rico M.H ◽  
Mersi Suriani Sinaga
Keyword(s):  
Fatty Acid ◽  
Volume Ratio ◽  
Biodiesel Production ◽  
Extraction Time ◽  
Glycerol Concentration ◽  
Purification Process ◽  
Soap Content ◽  
Test Volume ◽  
Base Content ◽  
Water Salt

Glycerol as a byproduct of biodiesel production was approximately formed 10% of the biodiesel weight. Impurities which contained in the glycerol such as catalyst, soap, methanol, water, salt, and matter organic non glycerol (MONG) have a significant effect on the glycerol concentration. So, it is necessary to treat the impurities. The purpose of this study is to know the effect of chloroform to glycerol purification process with acidification method using hydrochloric acid as pretreatment process. This research was begun with acid addition to the glycerol to neutralize the base content and to split the soap content into free fatty acid and salt, that are more easily separated from glycerol. Then the process was continued with extraction by the solvent chloroform using the variable of test volume ratio (v/v) (1:1, 1:1.5, 1:2)  and the extraction time (20, 40, and 60 minutes). The results showed that the more volume of solvent used, gave less extraction time to produce high purity of glycerol. The highest purity produced in this study amounted to 90,9082% is obtained at the ratio of the volume solvent (v/v) 1:1 with extraction time 60 minutes.

Constraints in Rice Production - Strategy to Overcome

2012 ◽  
Vol 3 (6) ◽  
pp. 9-11
Author(s):  
N. Srihari Narayana ◽  
◽  
V. Sailaja V. Sailaja ◽  
P.V. Satyagopal P.V. Satyagopal ◽  
S.V. Prasad S.V. Prasad
Keyword(s):  
Rice Production ◽  
Production Strategy

AGRICULTURAL PRODUCTION OF CA DONG PEOPLE IN RESETTLEMENT AREA OF TRANH RIVER HYDROPOWER NUMBER 2: CURRENT SITUATION AND IMPACT FACTORS

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Bùi Thị Bích Lan
Keyword(s):  
Social Relations ◽  
Agricultural Production ◽  
Large Scale ◽  
Transition Process ◽  
Impact Factors ◽  
Small Scale ◽  
Scale Production ◽  
Technical Application ◽  
Production Models ◽  
Role Of The State

In Vietnam, the construction of hydropower projects has contributed significantly in the cause of industrialization and modernization of the country. The place where hydropower projects are built is mostly inhabited by ethnic minorities - communities that rely primarily on land, a very important source of livelihood security. In the context of the lack of common productive land in resettlement areas, the orientation for agricultural production is to promote indigenous knowledge combined with increasing scientific and technical application; shifting from small-scale production practices to large-scale commodity production. However, the research results of this article show that many obstacles in the transition process are being posed such as limitations on natural resources, traditional production thinking or the suitability and effectiveness of scientific - technical application models. When agricultural production does not ensure food security, a number of implications for people’s lives are increasingly evident, such as poverty, preserving cultural identity, social relations and resource protection. Since then, it has set the role of the State in researching and building appropriate agricultural production models to exploit local strengths and ensure sustainability.

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