Development of a novel adenovirus purification process utilizing selective precipitation of cellular DNA

2005 ◽  
Vol 91 (1) ◽  
pp. 12-21 ◽  
Author(s):  
Aaron R. Goerke ◽  
Brian C.S. To ◽  
Ann L. Lee ◽  
Sangeetha L. Sagar ◽  
John O. Konz
Keyword(s):  
Purification Process ◽  
Selective Precipitation ◽  
Cellular Dna

Gram-scale production of sugar nucleotides and their derivatives

2021 ◽  
Author(s):  
Shuang Li ◽  
shuaishuai wang ◽  
Yaqian Wang ◽  
Jingyao Qu ◽  
Xianwei Liu ◽  
...  
Keyword(s):  
Scale Production ◽  
Purification Process ◽  
Selective Precipitation ◽  
Sugar Nucleotides ◽  
Production Strategy

Here, we report a practical sugar nucleotides production strategy that combined high-concentrated multienzyme catalyzed reaction and a robust chromatography-free selective precipitation purification process. Twelve sugar nucleoitdes were synthesized at gram-scale...

A replicative form of the DNA of minute virus of mice

1973 ◽  
Vol 19 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Phyllis R. Dobson ◽  
C. W. Helleiner
Keyword(s):  
Sodium Dodecylsulfate ◽  
Plaque Assay ◽  
Progeny Virus ◽  
Sedimentation Rates ◽  
Free Virus ◽  
Minute Virus Of Mice ◽  
Selective Precipitation ◽  
L Cells ◽  
Minute Virus ◽  
Cellular Dna

The growth of minute virus of mice (MVM) in L cells was followed by plaque assay of both cell-associated and free virus at intervals up to 36 h after infection. The major production of progeny virus occurred after incubation for 27 h and most of the virus remained cell-associated. L cells infected with MVM were pulse-labeled with 3H-thymidine for 6 h preceding induction of lysis with sodium dodecylsulfate. Two new species of DNA remained in the supernatant fractions from lysates after selective precipitation by 1 M NaCl of cellular DNA. These first appeared 17 h after infection. On the basis of sedimentation rates and response to heating and to treatment with alkali, one of these species is a hydrogen-bonded duplex molecule, which on denaturation hybridized with the single-stranded DNA of MVM virions.

Analysis of galanthamine and related alkaloids in plant extracts and during the purification process by GC-MS

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
S Berkov ◽  
C Codina ◽  
F Viladomat ◽  
J Bastida
Keyword(s):  
Plant Extracts ◽  
Purification Process

PENGARUH PELARUT KLOROFORM DALAM PEMURNIAN GLISEROL DENGAN PROSES ASIDIFIKASI ASAM KLORIDA

2016 ◽  
Vol 5 (3) ◽  
pp. 38-43
Author(s):  
Windi Monica Surbakti ◽  
Gerson Rico M.H ◽  
Mersi Suriani Sinaga
Keyword(s):  
Fatty Acid ◽  
Volume Ratio ◽  
Biodiesel Production ◽  
Extraction Time ◽  
Glycerol Concentration ◽  
Purification Process ◽  
Soap Content ◽  
Test Volume ◽  
Base Content ◽  
Water Salt

Glycerol as a byproduct of biodiesel production was approximately formed 10% of the biodiesel weight. Impurities which contained in the glycerol such as catalyst, soap, methanol, water, salt, and matter organic non glycerol (MONG) have a significant effect on the glycerol concentration. So, it is necessary to treat the impurities. The purpose of this study is to know the effect of chloroform to glycerol purification process with acidification method using hydrochloric acid as pretreatment process. This research was begun with acid addition to the glycerol to neutralize the base content and to split the soap content into free fatty acid and salt, that are more easily separated from glycerol. Then the process was continued with extraction by the solvent chloroform using the variable of test volume ratio (v/v) (1:1, 1:1.5, 1:2)  and the extraction time (20, 40, and 60 minutes). The results showed that the more volume of solvent used, gave less extraction time to produce high purity of glycerol. The highest purity produced in this study amounted to 90,9082% is obtained at the ratio of the volume solvent (v/v) 1:1 with extraction time 60 minutes.

Extracellular polysaccharides from suspension cultures of Nicotiana plumbaginifolia

2020 ◽  
Author(s):  
Ian Sims ◽  
A Bacic
Keyword(s):  
Uronic Acid ◽  
Cell Suspension Cultures ◽  
Anion Exchange Chromatography ◽  
Nicotiana Plumbaginifolia ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Selective Precipitation ◽  
The Individual

The soluble polymers secreted by cell-suspension cultures of Nicotiana plumbaginifolia contained 78% carbohydrate, 6% protein and 4% inorganic material. The extracellular polysaccharides were separated into three fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7 and the individual polysaccharides in each fraction were then isolated by selective precipitation and enzymic treatment. Monosaccharide and linkage compositions were determined for each polysaccharide after reduction of uronic acid residues and the degree of esterification of the various uronic acid residues in each polysaccharide was determined concurrently with the linkage types. Six components were identified: an arabinoxyloglucan (comprising 34% of the total polysaccharide) and a galactoglucomannan (15%) in the unbound neutral fraction, a type II arabinogalactan (an arabinogalactan-protein, 11%) and an acidic xylan (3%) in the first bound fraction, and an arabinoglucuronomannan (11%) and a galacturonan (26%) in the second bound fraction. © 1995.

PEG1000 as a Low Melting and Ecofriendly Solvent for Air Oxidative and Catalyst Free 2,4-Disubstitute Quinazoline Synthesis

2020 ◽  
Vol 17 (9) ◽  
pp. 709-716
Author(s):  
Ebrahim Saeedian Moghadam ◽  
Shahrzad Ghafary ◽  
Mohsen Amini
Keyword(s):  
Melting Point ◽  
High Yield ◽  
Purification Process ◽  
Poly Ethylene Glycol ◽  
Free Condition ◽  
Catalyst Free ◽  
Privileged Scaffold ◽  
Quinazoline Derivatives ◽  
Poly Ethylene ◽  
Work Up

With regard to the importance of quinazoline as a privileged scaffold, herein we report the synthesis of twenty seven 2,4-disubstitute quinazoline derivatives in a new catalyst free condition. In the current work, poly ethylene glycol (PEG1000) as an inexpensive, very simple commercially available, ecofriendly and low melting point solvent was used. Air bubbling, a green oxidant, for oxidation purpose was also used. This is the first report about using PEG1000 as a solvent simultaneously with air bubbling as oxidant in quinazoline synthesis. All of the compounds 1-27 were synthesized in high yield with very simple work up and purification process without using column chromatography. All the structures were confirmed using 1H NMR, 13C NMR, IR, MS and elemental analysis.

Rearrangement of a common cellular DNA domain on chromosome 4 in human primary liver tumors.

1988 ◽  
Vol 62 (2) ◽  
pp. 629-632 ◽  
Author(s):  
C Pasquinelli ◽  
F Garreau ◽  
L Bougueleret ◽  
E Cariani ◽  
K H Grzeschik ◽  
...  
Keyword(s):  
Liver Tumors ◽  
Chromosome 4 ◽  
Primary Liver Tumors ◽  
Cellular Dna
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