scholarly journals Correction: Nutritional targeting modification of silkworm pupae oil catalyzed by a smart hydrogel immobilized lipase

2021 ◽  
Author(s):  
Jin-Zheng Wang ◽  
Cheng-Kun Wu ◽  
Cheng-Hai Yan ◽  
Huan Chen ◽  
Shuai You ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Smart Hydrogel ◽  
Silkworm Pupae

Correction for ‘Nutritional targeting modification of silkworm pupae oil catalyzed by a smart hydrogel immobilized lipase’ by Jin-Zheng Wang et al., Food Funct., 2021, 12, 6240–6253, DOI: 10.1039/D1FO00913C.

Nutritional targeting modification of silkworm pupae oil catalyzed by a smart hydrogel immobilized lipase

2021 ◽  
Author(s):  
Jin-Zheng Wang ◽  
Cheng-Kun Wu ◽  
Cheng-Hai Yan ◽  
Huan Chen ◽  
Shuai You ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Nutritional Supplement ◽  
Relative Content ◽  
Smart Hydrogel ◽  
Microfluidic Reactor ◽  
Silkworm Pupae ◽  
Silkworm Pupa

To prepare a nutritional supplement using silkworm pupa oil (SPO) as a feedstock, a microfluidic reactor with smart hydrogel immobilized lipase was firstly constructed to reduce the relative content of...

Aroma synthesis by immobilized lipase from Mucor sp.

2003 ◽  
Vol 32 (5) ◽  
pp. 589-595 ◽  
Author(s):  
Houria Abbas ◽  
Louis Comeau
Keyword(s):  
Immobilized Lipase

scholarly journals Author Correction: Observation of morphological abnormalities in silkworm pupae after feeding 137CsCl-supplemented diet to evaluate the effects of low dose-rate exposure

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sota Tanaka ◽  
Tadatoshi Kinouchi ◽  
Tsuguru Fujii ◽  
Tetsuji Imanaka ◽  
Tomoyuki Takahashi ◽  
...  
Keyword(s):  
Dose Rate ◽  
Low Dose ◽  
Morphological Abnormalities ◽  
Low Dose Rate ◽  
Silkworm Pupae

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Oral Vaccination of Grass Carp (Ctenopharyngodon idella) with Baculovirus-Expressed Grass Carp Reovirus (GCRV) Proteins Induces Protective Immunity against GCRV Infection

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 41
Author(s):  
Changyong Mu ◽  
Qiwang Zhong ◽  
Yan Meng ◽  
Yong Zhou ◽  
Nan Jiang ◽  
...  
Keyword(s):  
Survival Rate ◽  
Grass Carp ◽  
Oral Vaccine ◽  
Serum Antibody ◽  
Ctenopharyngodon Idella ◽  
Control Group ◽  
Oral Vaccination ◽  
Grass Carp Reovirus ◽  
Silkworm Pupae ◽  
Grass Carp Ctenopharyngodon Idella

The grass carp reovirus (GCRV) causes severe hemorrhagic disease with high mortality and leads to serious economic losses in the grass carp (Ctenopharyngodon idella) industry in China. Oral vaccine has been proven to be an effective method to provide protection against fish viruses. In this study, a recombinant baculovirus BmNPV-VP35-VP4 was generated to express VP35 and VP4 proteins from GCRV type Ⅱ via Bac-to-Bac baculovirus expression system. The expression of recombinant VP35-VP4 protein (rVP35-VP4) in Bombyx mori embryo cells (BmE) and silkworm pupae was confirmed by Western blotting and immunofluorescence assay (IFA) after infection with BmNPV-VP35-VP4. To vaccinate the grass carp by oral route, the silkworm pupae expressing the rVP35-VP4 proteins were converted into a powder after freeze-drying, added to artificial feed at 5% and fed to grass carp (18 ± 1.5 g) for six weeks, and the immune response and protective efficacy in grass carp after oral vaccination trial was thoroughly investigated. This included blood cell counting and classification, serum antibody titer detection, immune-related gene expression and the relative percent survival rate in immunized grass carp. The results of blood cell counts show that the number of white blood cells in the peripheral blood of immunized grass carp increased significantly from 14 to 28 days post-immunization (dpi). The differential leukocyte count of neutrophils and monocytes were significantly higher than those in the control group at 14 dpi. Additionally, the number of lymphocytes increased significantly and reached a peak at 28 dpi. The serum antibody levels were significantly increased at Day 14 and continued until 42 days post-vaccination. The mRNA expression levels of immune-related genes (IFN-1, TLR22, IL-1β, MHC I, Mx and IgM) were significantly upregulated in liver, spleen, kidney and hindgut after immunization. Four weeks post-immunization, fish were challenged with virulent GCRV by intraperitoneal injection. The results of this challenge study show that orally immunized group exhibited a survival rate of 60% and relative percent survival (RPS) of 56%, whereas the control group had a survival rate of 13% and RPS of 4%. Taken together, our results demonstrate that the silkworm pupae powder containing baculovirus-expressed VP35-VP4 proteins could induce both non-specific and specific immune responses and protect grass carp against GCRV infection, suggesting it could be used as an oral vaccine.

Self-adhesive PMIA membranes with virus-like silica immobilized lipase for efficient biological aging of Chinese liquor

2021 ◽  
Vol 621 ◽  
pp. 118990
Author(s):  
Zhen Chen ◽  
Huan-Yin Xie ◽  
Gui-E Chen ◽  
Sun-Jie Xu ◽  
Zhen-Liang Xu ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Biological Aging ◽  
Chinese Liquor

scholarly journals Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 704
Author(s):  
Qian Yu ◽  
Pengfei Chang ◽  
Xiaoxuan Liu ◽  
Peng Lü ◽  
Qi Tang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bombyx Mori ◽  
Nuclear Polyhedrosis Virus ◽  
Expression System ◽  
Efficient Production ◽  
Human Bocavirus ◽  
Adeno Associated Virus ◽  
Silkworm Pupae ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

Recombinant adeno-associated virus (AAV) vectors have broad application prospects in the field of gene therapy. The establishment of low-cost and large-scale manufacturing is now the general agenda for industry. The baculovirus-insect cell/larva expression system has great potential for these applications due to its scalability and predictable biosafety. To establish a more efficient production system, Bombyx mori pupae were used as a new platform and infected with recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV). The production of a chimeric recombinant adeno-associated virus (rAAV) serotype 2/human bocavirus type-1 (HBoV1) vector was used to evaluate the efficiency of this new baculovirus expression vector (BEV)–insect expression system. For this purpose, we constructed two recombinant BmNPVs, which were named rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP. The yields of rAAV2/HBoV1 derived from the rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP co-infected BmN cells exceeded 2 × 104 vector genomes (VG) per cell. The rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP can express stably for at least five passages. Significantly, rAAV2/HBoV1 could be efficiently generated from BmNPV-infected silkworm larvae and pupae at average yields of 2.52 × 1012 VG/larva and 4.6 × 1012 VG/pupa, respectively. However, the vectors produced from the larvae and pupae had a high percentage of empty particles, which suggests that further optimization is required for this platform in the future. Our work shows that silkworm pupae, as an efficient bioreactor, have great potential for application in the production of gene therapy vectors.

An integrated liquid crystal sensing device assisted by the surfactant-embedded smart hydrogel

2021 ◽  
pp. 113313
Author(s):  
Jiantao Ping ◽  
Lubin Qi ◽  
Quanbo Wang ◽  
Shuhua Liu ◽  
Yifei Jiang ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Smart Hydrogel
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